Author(s):
Aamir Malik, Sandip Firke, Ravindra Patil, Atul Shirkhedkar, Mohan Kalaskar
Email(s):
sandipfirke@rediffmail.com
DOI:
10.5958/2231-5675.2019.00011.5 
Address:
Aamir Malik1, Sandip Firke2*, Ravindra Patil2, Atul Shirkhedkar2, Mohan Kalaskar
1Research Scholar, Department, R. C. Patel Institute of Pharmaceutical Education and Research, Shipur, Dhule (425405)
2Professor, Pharmaceutical Chemistry Department, R. C. Patel Institute of Pharmaceutical Education and Research, Shipur, Dhule (425405)
*Corresponding Author
Published In:
Volume - 9,
Issue - 2,
Year - 2019
ABSTRACT:
A simple, accurate and precise spectrophotometric method including area under curve has been developed for the estimation of Deferiprone (DEF) in bulk material and capsule dosage form. Deferiprone is an iron chelating agent, used in the treatment of thalassemia syndromes when iron overload due to blood transfusions occurs. Four simple UV Spectrophotometric methods were established for determination of Deferiprone, using double beam UV spectrophotometer (UV-2450, Shimadzu, Japan). Deferiprone has absorbance maxima at 278 nm with distilled water used as a solvent. Deferiprone obeys Beer-Lambert’s law in the concentration range of 2-10 µg/mL. The % recovery by proposed methods was found to be 98-101%. The Precision studies show less than 2 in terms of %RSD indicates precise nature of proposed method. All methods were validated as per the ICH guidelines, Q2 (R1). The statistical data and results prove that developed methods are simple, accurate and precise and can be used for the routine analysis of DEF in bulk and pharmaceutical formulation.
Cite this article:
Aamir Malik, Sandip Firke, Ravindra Patil, Atul Shirkhedkar, Mohan Kalaskar. Development and Validation of Zero and First-Order Derivative Area under Curve Spectrophotometric Methods for the Determination of Deferiprone in Bulk Material and Capsules. Asian J. Pharm. Ana. 2019; 9(2):49-54. doi: 10.5958/2231-5675.2019.00011.5
Cite(Electronic):
Aamir Malik, Sandip Firke, Ravindra Patil, Atul Shirkhedkar, Mohan Kalaskar. Development and Validation of Zero and First-Order Derivative Area under Curve Spectrophotometric Methods for the Determination of Deferiprone in Bulk Material and Capsules. Asian J. Pharm. Ana. 2019; 9(2):49-54. doi: 10.5958/2231-5675.2019.00011.5 Available on: https://ajpaonline.com/AbstractView.aspx?PID=2019-9-2-2