Author(s):
Pravin J. Kuwar, Sunila A. Patil, Sunil P. Pawar
Email(s):
pravinjrajput12@gmail.com
DOI:
10.52711/2231-5675.2026.00038 
Address:
Pravin J. Kuwar, Sunila A. Patil*, Sunil P. Pawar*
Department of Pharmaceutical Quality Assurance, P.S.G.V.P. M’s, College of Pharmacy, Shahada, 425409, Maharashtra, India.
*Corresponding Author
Published In:
Volume - 16,
Issue - 3,
Year - 2026
ABSTRACT:
One of the most popular analytical methods for the separation, identification, and measurement of pharmaceutical substances is High Performance Liquid Chromatography (HPLC). The concepts, kinds, method development, and validation elements of HPLC are the main topics of this review, with a particular focus on the analysis of mycophenolic acid (MPA), a significant immunosuppressive medication. The mechanics of separation for several chromatographic modes, including as adsorption, ion-exchange, and size-exclusion chromatography, are covered. The review focuses on various reported RP-HPLC and HPLC-MS/MS techniques for biological matrices, pharmaceutical dosage forms, and bulk MPA quantification. A summary of important analytical parameters is provided, including mobile phase composition, column selection, flow rate, detection wavelength, linearity, accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ). To guarantee specificity and dependability, stability-indicating techniques and forced degradation investigations are also emphasised. In order to guarantee method robustness and reproducibility, validation requirements are described in accordance with ICH guidelines. Researchers and analysts in pharmaceutical quality control and bioanalytical studies may find this review's thorough overview of HPLC method development and its use in the quantitative detection of mycophenolic acid helpful.
Cite this article:
Pravin J. Kuwar, Sunila A. Patil, Sunil P. Pawar. Development and Validation of Stability Indicating RP-HPLC Method for Assay of Mycophenolic Acid in Tablet Dosage Form. Asian Journal of Pharmaceutical Analysis. 2026; 16(3):244-8. doi: 10.52711/2231-5675.2026.00038
Cite(Electronic):
Pravin J. Kuwar, Sunila A. Patil, Sunil P. Pawar. Development and Validation of Stability Indicating RP-HPLC Method for Assay of Mycophenolic Acid in Tablet Dosage Form. Asian Journal of Pharmaceutical Analysis. 2026; 16(3):244-8. doi: 10.52711/2231-5675.2026.00038 Available on: https://ajpaonline.com/AbstractView.aspx?PID=2026-16-3-14
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