MENU

Author(s): Srinivasa Reddy, Nirmala Nayak, Imran Ahmed, Licto Thomas, Arindam Mukhopadhyay, Saral Thangam

Email(s): arindam.mukhopadhyay@norwichclinical.com

DOI: 10.5958/2231-5675.2016.00014.4   

Address: Srinivasa Reddy, Nirmala Nayak, Imran Ahmed, Licto Thomas, Arindam Mukhopadhyay*, Saral Thangam
Norwich Clinical Services Pvt Ltd, 147/F, 8th Main, 3rd Block, Koramangala, Bangalore 560034, India
*Corresponding Author

Published In:   Volume - 6,      Issue - 2,     Year - 2016


ABSTRACT:
Oseltamivir phosphate is licensed for the treatment of patients with influenza virus infection. Two LCMS/MS methods for simultaneous quantification of Oseltamivir and its active metabolite, oseltamivir carboxylate in human plasma were described here. After solid phase extraction sample was separated either on a reversed phase C18 column with a stepwise gradient using 0.05% formic acid and methanol or a cation-exchange column using an isocratic mobile phase (7 mM Ammonium formate, pH 3.5 ± 0.2: Methanol: 50:50, v/v). Flow rate of 1 mL/min was maintained in both cases. A triple quadrupole mass spectrometer operating in the positive ionization mode was used for detection and drug quantification. Both methods were validated over a range of 0.52ng/ml to 207.00 ng/ml for Oseltamivir and 4.08 ng/ml to 1200.00 ng/ml for Oseltamivir Carboxylate. Deuterated Oseltamivir and Oseltamivir carboxylate were used as internal standards. The accuracies and precisions for Oseltamivir were between 91-102% and 0.9 – 13.7% for all concentration levels. The accuracies and precisions for Oseltamivir carboxylate were between 88-109% and 0.5 – 8.2% at all levels. Furthermore, Oseltamivir and its metabolite were stable in plasma ex vivo for at least 191 days when stored at -20°C or below.


Cite this article:
Srinivasa Reddy, Nirmala Nayak, Imran Ahmed, Licto Thomas, Arindam Mukhopadhyay, Saral Thangam. Development and Validation of two LCMS/MS Methods for Simultaneous Estimation of Oseltamivir and its Metabolite in Human Plasma and Application in Bioequivalence Study. Asian J. Pharm. Ana. 2016; 6(2): 91-101. doi: 10.5958/2231-5675.2016.00014.4

Cite(Electronic):
Srinivasa Reddy, Nirmala Nayak, Imran Ahmed, Licto Thomas, Arindam Mukhopadhyay, Saral Thangam. Development and Validation of two LCMS/MS Methods for Simultaneous Estimation of Oseltamivir and its Metabolite in Human Plasma and Application in Bioequivalence Study. Asian J. Pharm. Ana. 2016; 6(2): 91-101. doi: 10.5958/2231-5675.2016.00014.4   Available on: https://ajpaonline.com/AbstractView.aspx?PID=2016-6-2-5


Recomonded Articles:

Author(s): K. Pramod, Shahid H. Ansari, Javed Ali

DOI:         Access: Open Access Read More

Author(s): Saroj Kumar Raul, Gopal Krishna Padhy, Pramudula Ramya Krishna, Boddu Uma Madhu Priya

DOI:         Access: Open Access Read More

Author(s): Saroj Kumar Raul, Bukkuru Spandana, Patibandla Sameera, Vegiraju Vikitha

DOI:         Access: Open Access Read More

Author(s): B. Thangabalan, M. Salomi, N. Sunitha, S. Manohar Babu

DOI:         Access: Open Access Read More

Author(s): P. Janaki Pathi, N. Appala Raju, G.Parvathalu

DOI:         Access: Open Access Read More

Author(s): Prashanthi. Y, Tentu Nageswara Rao, Yellapu Srinivas

DOI: 10.5958/2231-5675.2018.00034.0         Access: Open Access Read More

Author(s): Hamid Khan, Mushir Ali, Alka Ahuja, Javed Ali

DOI:         Access: Open Access Read More

Author(s): Priyanga Rangasamy, Vadakkenchery Salimudheen Hansiya, Palanisamy Uma Maheswari, Thamburaj Suman, Natesan Geetha

DOI: 10.5958/2231-5675.2019.00014.0         Access: Open Access Read More

Author(s): Srinivasa Reddy, Nirmala Nayak, Imran Ahmed, Licto Thomas, Arindam Mukhopadhyay, Saral Thangam

DOI: 10.5958/2231-5675.2016.00014.4         Access: Open Access Read More

Author(s): Sathish Kumar Konidala, Pampana V V Suresh Babu , Ranj, K S D Ranjitha

DOI: 10.5958/2231-5675.2016.00011.9         Access: Open Access Read More

Author(s): Md. Ahsanul Haque, Mohammad Shahriar, Most. Nazma Parvin, S. M. Ashraful Islam

DOI:         Access: Open Access Read More

Author(s): Saravanan. R, Bharani Pandilla, Vijayageetha. R, Kavitha. M, Ashok. P

DOI: 10.5958/2231-5675.2021.00010.         Access: Open Access Read More

Author(s): Khushbu K. Patel, Arati M. Patel, C. N. Patel

DOI: 10.52711/2231-5675.2021.00031         Access: Open Access Read More

Asian Journal of Pharmaceutical Analysis (AJPA) is an international, peer-reviewed journal, devoted to pharmaceutical analysis...... Read more >>>

RNI: Not Available                     
DOI: 10.5958/2231–5675 


Recent Articles




Tags