Author(s):
Panikumar Durga Anumolu, Syed Sara Afreen, Ashok Gorja, Devilal Jarpula, Pulusu Veera Shakar, Rahul Nalluri
Email(s):
saraafreen2422@gmail.com
DOI:
10.52711/2231-5675.2024.00045 
Address:
Panikumar Durga Anumolu1, Syed Sara Afreen1*, Ashok Gorja1, Devilal Jarpula3, Pulusu Veera Shakar2, Rahul Nalluri4
1Gokaraju Rangaraju College of Pharmacy, Department of Pharmaceutical Analysis, Osmania University, Hyderabad, Telangana - 500090, India.
2Ohio University, Department of Chemistry and Bio-chemistry, Athens, OH, USA-45701.
3Gokaraju Rangarajan College of Pharmacy Department of Pharmaceutical Chemistry, Osmania University, Hyderabad, Telangana-500090, India.
4Independent Researcher.
*Corresponding Author
Published In:
Volume - 14,
Issue - 4,
Year - 2024
ABSTRACT:
The simultaneous quantification of paracetamol (PAR) and flupirtine maleate (FLU) was accomplished with a precise and accurate stability-indicating reversed phase high performance liquid chromatographic method. This method used an ACE C18 (250 × 4.6mm, 5µ) column and a mobile phase made of methanol: phosphate buffer, pH 7.5 (40: 60, v/v), pumped at a flow rate of 1.0mL min-1. Quantification was accomplished using photodiode array (PDA) detection at 250nm. It was discovered that the retention periods for PAR and FLU were, respectively, 3.09 and 4.54 minutes. For PAR and FLU, linearity was confirmed in the range of 32.5 - 95.7µg mL-1 and 10 - 30µg mL-1, respectively. Both medications underwent oxidation, neutral hydrolysis, acid-alkali, and dry heat degradation; the stressed samples. At their Rt values, the degraded peaks showed a considerable divergence from the analyte peak that could be well resolved. Method validation followed ICH norms. It was discovered that the planned and verified stability-indicating HPLC approach was straightforward, sensitive, and repeatable for the measurement of the medications under study and the byproducts of their degradation in bulk and commercial goods.
Cite this article:
Panikumar Durga Anumolu, Syed Sara Afreen, Ashok Gorja, Devilal Jarpula, Pulusu Veera Shakar, Rahul Nalluri. Stability-Indicating RP-HPLC Method for Simultaneous Quantification of Paracetamol and Flupirtine Maleat. Asian Journal of Pharmaceutical Analysis. 2024; 14(4):251-5. doi: 10.52711/2231-5675.2024.00045
Cite(Electronic):
Panikumar Durga Anumolu, Syed Sara Afreen, Ashok Gorja, Devilal Jarpula, Pulusu Veera Shakar, Rahul Nalluri. Stability-Indicating RP-HPLC Method for Simultaneous Quantification of Paracetamol and Flupirtine Maleat. Asian Journal of Pharmaceutical Analysis. 2024; 14(4):251-5. doi: 10.52711/2231-5675.2024.00045 Available on: https://ajpaonline.com/AbstractView.aspx?PID=2024-14-4-9
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