Author(s): Suraj J. Patil, Sneha S. Kharat, Saniket B. Wadgaonkar

Email(s): snehakharat191@gmail.com

DOI: 10.52711/2231-5675.2024.00029   

Address: Suraj J. Patil, Sneha S. Kharat, Saniket B. Wadgaonkar
Department of Pharmaceutical Chemistry, Annasaheb Dange College of B. Pharmacy, Ashta, Sangli 416416, Maharashtra, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 3,     Year - 2024


ABSTRACT:
This work presents UV spectroscopy-based UDCA development and validation. For the estimation of ursodeoxycholic, a quick, accurate, and affordable UV-spectroscopic approach has been established. Ursodeoxycholic acid was discovered to have an absorption maximum in methanol of 205nm. With a correlation coefficient of 0.983, the medication exhibits linear behaviour in the concentration ranges of 2 – 10g/ml at 205 nm. The recovery experiment, which was carried out at three levels of 80%, 100%, and 120%, was used to evaluate the method's accuracy. The percent recovery was discovered to be between 90% and 120%. Low percentages of RSD are a sign of a method's accuracy and reproducibility. The %RSD 2 shows that the procedure is accurate. Less than 2% was robust and rugged, which is in-acceptable range3.


Cite this article:
Suraj J. Patil, Sneha S. Kharat, Saniket B. Wadgaonkar. Method Development and Validation of Ursodeoxycholic Acid by UV Spectroscopy. Asian Journal of Pharmaceutical Analysis. 2024; 14(3):161-5. doi: 10.52711/2231-5675.2024.00029

Cite(Electronic):
Suraj J. Patil, Sneha S. Kharat, Saniket B. Wadgaonkar. Method Development and Validation of Ursodeoxycholic Acid by UV Spectroscopy. Asian Journal of Pharmaceutical Analysis. 2024; 14(3):161-5. doi: 10.52711/2231-5675.2024.00029   Available on: https://ajpaonline.com/AbstractView.aspx?PID=2024-14-3-10


REFERENCE:
1.    https://en.m.Wikipedia.org/Wiki/Ursodiol.
2.    https://en.wikipedia.org/wiki/Ultraviolet%E2%80%93visible_spectroscopy
3.    Asha Devi, Abhishek Soni, Amit Chaudhary. Development and Validation of the UV Spectrophotometric Method of Ursodeoxycholic Acid in Methanol. J Med Scie and Cli Res., 2019; 7(10): 867-69.
4.    Hsing-Yun Chang, Ching-Hua Kuo, Shao-Wen Sun. Determination of Ursodeoxycholic Acid in Pharmaceutical Preparations by Capillary Electrophoresis with Indirect UV Detection. J Pharm Biomed Anal., 2003; 32: 949-56.
5.    Hausherr A, Roessle C, Pinet E, Vasseur V, Abarou T, Benakouche S, Bourdon O, Storme T (). Development and validation of a new HPLC method for the analysis of a novel oral suspension formulation of 50 mg/ml ursodeoxycholic acid for new borns. Pharmaceutical Technology in Hospital Pharmacy. 2020; 1: 5(1).
6.    J. Mukherjee, T. K. Pal. Development and Validation of RP-HPLC Method to Determine Ursodeoxycholic Acid in Pharmaceutical Dosage forms. Int J Pharm Sci Res. 2011; 2(1): 73-78.
7.    M.Ganesan, S. Nanjundan, S. Viswanathan, G. Uma. Liquid Chromatography/Tandem Mass Spectrometry for the Simultaneous Determination of Ursodiol and its Major Metabolites, Tauroursodeoxycholic Acid and Glycoursodeoxycholic Acid in Human Plasma. E-J Chem., 2012; 9(3): 1605-12.
8.    Najmul Hasan, Mathurot Chaiharn, Tanveer Abbas, Sikandar Khan Sherwani, Samal Mukayeva, Shabana Naz Shah, Abdur Raheem and Ameer Shahid. Development and Validation of RP-LC-UV Method for Determination of Ursodeoxycholic Acid in Capsule and Human Serum. World App Sci J., 2014; 32 (4): 560-66.
9.    Oriana Boscolo, Sabrina Flor, Cecilia Dobrecky, Leandro Salvo, Valeria Tripodi, Silvia Lucangioli. Development and validation of a LC-UV Method Applied to the Quality Control of Ursodeoxycholic Acid in Raw Material and Pharmaceutical Formulations. IOSR J Pharm. 2017; 7(2): 111-16.
10.    Sonu Sundd Singh, Hiten Shah, Sapna Gupta, Manish Jain, Kuldeep Sharma, Harshvardhan Patel, Bhavin Shah, Purav Thakkar, Nimesh Patel, Ruchy Shah and Braj BgushanLohary. Validation of LC/MS Electrospray Ionisation Method for the of Ursodiol in Human Plasma and its Application in Bioequivalence Study. Zydus Res Centre, 2004; 94: 1-9.
11.    Sneha Singh, Mohit Saini, Amit Kumar, Kanika Manral, Sonam Joshi. Method Development and Validation for Estimation of Ursodeoxycholic Acid in Tablet Dosage form by HPLC. Asi J Res Reports in Gastroenterology. 2020; 3(3): 14-23.
12.    Yash Devendrabhai Patel, Ashok Peepliwal, Chandrakant G. Bonde. Enhancement of Absorptivity of Ursodeoxycholic Acid. Bulletin Pharm Res. 2011; 1(2): 1-6.
13.    Zhaklina Poposka Svirkova, Zhaklina Poposka Svirkova, Zorica Arsova-Sarafinovska, Aleksandra Grozdanova. Optimization of RP-HPLC method with UV detection for determination of ursodeoxycholic acid in pharmaceutical formulations. Macedonian Pharmaceutical Bulletin. 2020; 66(1):  85-90.

Recomonded Articles:

Author(s): Dipti G. Phadtare, Pawar Amol R, R.B. Saudagar

DOI: 10.5958/2231-5675.2016.00019.3         Access: Closed Access Read More

Author(s): K. Pramod, Shahid H. Ansari, Javed Ali

DOI:         Access: Open Access Read More

Author(s): G. Krishnamoorthy, C. Diana Priyadarshini, R. Senthamarai

DOI:         Access: Open Access Read More

Author(s): Sushil D. Patil, Sayali K. Chaure, Maswood Ahmed Hafizur Rahman, Prajkta U. Varpe, Sanjay Kshirsagar.

DOI: 10.5958/2231-5675.2017.00004.7         Access: Open Access Read More

Author(s): Yogita R. Indalkar, Aloorkar N.H.

DOI: 10.5958/2231-5675.2015.00028.9         Access: Open Access Read More

Author(s): Pinaz A. Kasad, K.S. Muralikrishna

DOI:         Access: Open Access Read More

Author(s): S. Subramanya Raj Urs, Bindu M., Ramyashree D., Sowmya K. N.

DOI: 10.5958/2231-5675.2020.00034.4         Access: Open Access Read More

Author(s): Santosh Gada, Anand Kumar Y, Saritha, C. Mallikarjuna Setty

DOI: 10.5958/2231-5675.2018.00036.4         Access: Open Access Read More

Author(s): Kirthi A, Shanmugam R, Mohana Lakshmi S , Ashok Kumar CK, Padmini K, Shanti Prathyusha M, Shilpa V

DOI:         Access: Open Access Read More

Author(s): Saroj Kumar Raul, Bukkuru Spandana, Patibandla Sameera, Vegiraju Vikitha

DOI:         Access: Open Access Read More

Author(s): B. Thangabalan, M. Salomi, N. Sunitha, S. Manohar Babu

DOI:         Access: Open Access Read More

Author(s): P. Janaki Pathi, N. Appala Raju, G.Parvathalu

DOI:         Access: Open Access Read More

Author(s): Vinutha Kommineni, K.P.R. Chowdary, S.V.U.M. Prasad

DOI: 10.5958/2231-5675.2019.00018.8         Access: Open Access Read More

Asian Journal of Pharmaceutical Analysis (AJPA) is an international, peer-reviewed journal, devoted to pharmaceutical analysis...... Read more >>>

RNI: Not Available                     
DOI: 10.5958/2231–5675 


Recent Articles




Tags