Author(s):
Suraj J. Patil, Sneha S. Kharat, Saniket B. Wadgaonkar
Email(s):
snehakharat191@gmail.com
DOI:
10.52711/2231-5675.2024.00029 
Address:
Suraj J. Patil, Sneha S. Kharat, Saniket B. Wadgaonkar
Department of Pharmaceutical Chemistry, Annasaheb Dange College of B. Pharmacy, Ashta, Sangli 416416, Maharashtra, India.
*Corresponding Author
Published In:
Volume - 14,
Issue - 3,
Year - 2024
ABSTRACT:
This work presents UV spectroscopy-based UDCA development and validation. For the estimation of ursodeoxycholic, a quick, accurate, and affordable UV-spectroscopic approach has been established. Ursodeoxycholic acid was discovered to have an absorption maximum in methanol of 205nm. With a correlation coefficient of 0.983, the medication exhibits linear behaviour in the concentration ranges of 2 – 10g/ml at 205 nm. The recovery experiment, which was carried out at three levels of 80%, 100%, and 120%, was used to evaluate the method's accuracy. The percent recovery was discovered to be between 90% and 120%. Low percentages of RSD are a sign of a method's accuracy and reproducibility. The %RSD 2 shows that the procedure is accurate. Less than 2% was robust and rugged, which is in-acceptable range3.
Cite this article:
Suraj J. Patil, Sneha S. Kharat, Saniket B. Wadgaonkar. Method Development and Validation of Ursodeoxycholic Acid by UV Spectroscopy. Asian Journal of Pharmaceutical Analysis. 2024; 14(3):161-5. doi: 10.52711/2231-5675.2024.00029
Cite(Electronic):
Suraj J. Patil, Sneha S. Kharat, Saniket B. Wadgaonkar. Method Development and Validation of Ursodeoxycholic Acid by UV Spectroscopy. Asian Journal of Pharmaceutical Analysis. 2024; 14(3):161-5. doi: 10.52711/2231-5675.2024.00029 Available on: https://ajpaonline.com/AbstractView.aspx?PID=2024-14-3-10
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