Author(s):
Devshree Yashwantbhai Patel, Javesh Kashinath Patil, Harsha Vasudev Chaudhari, Ruchita M Kothari
Email(s):
devshree744@gmail.com
DOI:
10.52711/2231-5675.2024.00038 
Address:
Devshree Yashwantbhai Patel1, Javesh Kashinath Patil2, Harsha Vasudev Chaudhari3, Ruchita M Kothari4
1Department of Pharmaceutical Quality Assurance, P.S.G.V.P. Mandal’s College of Pharmacy, Shahada, Dist - Nandurbar, 425409, Maharashtra, India.
2Associate Professor, Department of Pharmaceutical Quality Assurance, P.S.G.V.P. Mandal’s College of Pharmacy, Shahada, Dist. - Nandurbar, 425409, Maharashtra, India.
3Department of Pharmaceutical Quality Assurance, P.S.G.V.P. Mandal’s College of Pharmacy, Shahada, Dist - Nandurbar, 425409, Maharashtra, India.
4Department of Pharmaceutical Quality Assurance, P.S.G.V.P. Mandal’s College of Pharmacy, Shahada, Dist - Nandurbar, 425409, India.
*Corresponding Author
Published In:
Volume - 14,
Issue - 4,
Year - 2024
ABSTRACT:
A Simple, precise and accurate RP-HPLC method was developed for determination of of Nitazoxanide in API and tablet dosage forms. The chromatographic separation was done on Cosmosil C18 Column (250mm x 4.6mm,5µm) using mobile phase MEOH+ 0.05% (OPA with TEA) Water (70+30% v/v) at flow rate 0.7ml/min and wavelength detection 340nm.The retention time for Nitazoxanide was found to be 4.333 min. Linearity was observed in the range10-50µg/ml (y=55.38x-37.01 and R² = 0.9976). The LOD and LOQ was found to be 0.4258 and 1.289 respectively. The Precision as indicated by repeatability study was having %RSD less than 2. The accuracy, intraday and interday were also found to be less than 2. Hence the proposed method is successfully developed and validated.
Cite this article:
Devshree Yashwantbhai Patel, Javesh Kashinath Patil, Harsha Vasudev Chaudhari, Ruchita M Kothari. Analytical Method Development and Validation of Nitazoxanide by RP-HPLC Method in API and Tablet Dosage Forms. Asian Journal of Pharmaceutical Analysis. 2024; 14(4):211-6. doi: 10.52711/2231-5675.2024.00038
Cite(Electronic):
Devshree Yashwantbhai Patel, Javesh Kashinath Patil, Harsha Vasudev Chaudhari, Ruchita M Kothari. Analytical Method Development and Validation of Nitazoxanide by RP-HPLC Method in API and Tablet Dosage Forms. Asian Journal of Pharmaceutical Analysis. 2024; 14(4):211-6. doi: 10.52711/2231-5675.2024.00038 Available on: https://ajpaonline.com/AbstractView.aspx?PID=2024-14-4-2
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