Author(s): Gandla Kumaraswamy, Gandla Lalitha, N. Ravindra, K.B. Pradeesha

Email(s): kumaraswamy.gandla@gmail.com

DOI: Not Available

Address: Gandla Kumaraswamy*, Gandla Lalitha, N. Ravindra, K.B. Pradeesha
Department of Pharmaceutical Analysis, Chilkur Balaji College of Pharmacy, Aziz Nagar –Hyderabad. 500075, Telangana.
*Corresponding Author

Published In:   Volume - 4,      Issue - 4,     Year - 2014


ABSTRACT:
A simple, accurate, rapid and precise isocratic reversed-phase high-performance liquid chromatographic method has been developed and validated for simultaneous determination of Amlodipine, Atenolol and Hydrochlorothiazide in capsules. The chromatographic separation was carried out on an cosmosil packed column 5C18-MS-I I analytical column (250×4.6 mm; 5 µm) with a mixture of Phosphate buffer:Acetonitrile:Methanol pH 6 adjusted with Ortho phosphoric acid (30:20:50, v/v) as mobile phase; at a flow rate of 1 ml/min. UV detection was performed at 240nm. The retention times were 2.637, 3.148 and 8.492min. for Hydrochlorothiazide, Atenolol and Amlodipine respectively. Calibration plots e linear (r2>0.998) over the concentration range 2-12µg/ml for Amlodipine , 10-60µg/ml Atenolol and 2-12µg/ml for Hydrochlorothiazide. The method was validated for accuracy, precision, specificity, linearity, and sensitivity. The proposed method was successfully used for quantitative analysis of capsules. No interference from any component of pharmaceutical dosage form was observed. Validation studies revealed that method is specific, rapid, reliable, and reproducible. The high recovery and low relative standard deviation confirm the suitability of the method for routine determination of Hydrochlorothiazide, Amlodipine and Atenolol in bulk drug and capsule dosage form.


Cite this article:
Gandla Kumaraswamy, Gandla Lalitha, N. Ravindra, K.B. Pradeesha. Simultaneous Estimation of Amlodipine, Atenolol and Hydrochlorothiazide in Bulk and Tablet Dosage Form by RP-HPLC Method. Asian J. Pharm. Ana. 4(4): Oct. - Dec. 2014; Page 131-136.


Recomonded Articles:

Author(s): Sushil D. Patil, Sunil V. Amurutkar, C.D. Upasani

DOI: 10.5958/2231-5675.2016.00030.2         Access: Open Access Read More

Author(s): Kirthi A, Shanmugam R, Mohana Lakshmi S , Ashok Kumar CK, Padmini K, Shanti Prathyusha M, Shilpa V

DOI:         Access: Open Access Read More

Author(s): Hamid Khan, Javed Ali

DOI: 10.5958/2231-5675.2017.00020.5         Access: Open Access Read More

Author(s): G. Krishnamoorthy, C. Diana Priyadarshini, R. Senthamarai

DOI:         Access: Open Access Read More

Author(s): Pinaz A. Kasad, K.S. Muralikrishna

DOI:         Access: Open Access Read More

Author(s): P. Janaki Pathi, N. Appala Raju, G.Parvathalu

DOI:         Access: Open Access Read More

Author(s): Paras Virani, Rajanit Sojitra, Hasumati Raj, Vineet Jain

DOI: 10.5958/2231-5675.2015.00025.3         Access: Open Access Read More

Author(s): Khushboo Patel*, Dhara Patel, Jaya Dwivedi, Priyanka Newal, Dhananjay Meshram

DOI: 10.5958/2231-5675.2017.00039.4         Access: Open Access Read More

Author(s): B. Thangabalan, M. Salomi, N. Sunitha, S. Manohar Babu

DOI:         Access: Open Access Read More

Author(s): Mansi J. Chaudhari, Suraj R. Chaudhari, Shailesh S. Chalikwar, Atul A. Shirkhedkar

DOI: 10.5958/2231-5675.2018.00008.X         Access: Open Access Read More

Author(s): Saroj Kumar Raul, Bukkuru Spandana, Patibandla Sameera, Vegiraju Vikitha

DOI:         Access: Open Access Read More

Author(s): S. Vidyadhara, RLC Sasidhar, B. Venkateswara Rao, P. Ratna Kumari

DOI:         Access: Open Access Read More

Author(s): Shobha Rani G, Lohita M, Jaya Preethi P, Madhavi R, Sunisitha B, Mounika D

DOI:         Access: Open Access Read More

Author(s): Madhavi R., Mohana Krishna A., Shobha Rani G., Mounika D.

DOI: 10.5958/2231-5675.2015.00008.3         Access: Open Access Read More

Asian Journal of Pharmaceutical Analysis (AJPA) is an international, peer-reviewed journal, devoted to pharmaceutical analysis...... Read more >>>

RNI: Not Available                     
DOI: 10.5958/2231–5675 

Popular Articles


Recent Articles




Tags