Author(s): Madhavi T. Bhosale, Pravin R. Dighe


DOI: 10.52711/2231-5675.2023.00033   

Address: Madhavi T. Bhosale, Pravin R. Dighe
Department of Pharmaceutical Chemistry, S.M.B.T College of Pharmacy, Nandi Hills, Dhamangaon, Tal - Igatpuri, Dist – Nashik.
*Corresponding Author

Published In:   Volume - 13,      Issue - 3,     Year - 2023

The hyphenated techniques are developed by combining two or three different analytical techniques. Mainly chromatographic techniques are combined with spectroscopic techniques. The separated components obtained from chromatographic techniques are then transmitted to spectroscopic detection technique through an interface. The improvements in hyphenated analytical methods in last two decades have significantly broadened their applications in the analysis of biomolecules especially in natural products. In this review article recent advancement in hyphenated techniques such as GC-MS, LC-MS, LC-NMR, MS-MS, LC-NMR-MS have been highlighted. The term ‘Hyphenated’ refers to separation and identification of the compounds. These techniques also show better analysis of sample compounds with better accuracy, precision and specificity. This review mainly focuses on the instrumental aspects of hyphenated techniques along with their interfaces. The interfaces such as Jet\Orifice separator interface, Electrospray interface, Atmospheric pressure chemical ionization interface have been covered. It has been concluded that these hyphenated techniques have become more advantageous for the analysis of drugs. This technique is useful for quality control, analytical research, impurity profiling and maintenance for human welfare and development.

Cite this article:
Madhavi T. Bhosale, Pravin R. Dighe. A Brief Review on Hyphenated Techniques. Asian Journal of Pharmaceutical Analysis. 2023; 13(3):205-9. doi: 10.52711/2231-5675.2023.00033

Madhavi T. Bhosale, Pravin R. Dighe. A Brief Review on Hyphenated Techniques. Asian Journal of Pharmaceutical Analysis. 2023; 13(3):205-9. doi: 10.52711/2231-5675.2023.00033   Available on:

1.    Hapse S.A., Bhagat B.V., Sawant P.D., R. Hyphenated Techniques: A Review. Intenational J. Pharm. Pharm. Res. 8, 33–44 (2017).
2.    Phalke, P. Kavade, S. Review on Hyphenated Techniques. Int. jouranl Chem. Stud. 2013;1: 157-165.
3.    Jose, B. E., Selvam, P. P. Identification of Phytochemical Constituents in the Leaf Extracts of Azima tetracantha Lam using Gas Chromatography-Mass Spectrometry (GC-MS) analysis and Antioxidant Activity. Asian J. Res. Chem. 2018; 11: 857.
4.    S. Ramchandran, M. Vamsikrishna, K. V. G. Assesment of cytotoxic activity of Agave cantula using Brine Shrimp (Artemia salina) Lethality Bioassay. Asian J. Sci. Res. 2011; 4: 90–94.
5.    Chinchole, R., Hatre, P. M., Desai, U. Chavan, R. Recent applications of hyphenated liquid chromatography techniques in forensic toxicology: A review. Int. J. Pharm. Sci. Rev. Res. 2012;  14: 57–63.
6.    Zavahir, J. S. et al. Relationships in Gas Chromatography — Fourier Transform Infrared Spectroscopy — Comprehensive and Multilinear Analysis.
7.    Korgaonkar, M. D. P. and D. Current Advance Analytical Techniques: A Review. Asian J. Res. Chem. 2009; 2: 235–238.
8.    Chauhan, A. Analytical and Bioanalytical Techniques GC-MS Technique and its Analytical Applications in Science and Technology. J. Anal. Bioanal. Tech. 2014; 5: 3-5.
9.    Chaturvedi K. K., Nanda R. K. Hyphenated gas chromatography. International Journal of Pharmaceutical Sciences Review and Research. 2010; 5; 18–27. (2010).
10.    Guo, X., Lankmayr, E. Hyphenated Techniques in Gas Chromatography. Adv. Gas Chromatogr. 2010; 2: 1–26.
11.    Banerjee S, Bonde CG, M. S. and P. Y. Advanced Hyphenated Techniques in Analytical Chemistry. Asian J. Res. Chem. 2009; 2: 380–387.
12.    Purcaro, G., Moret, S. Conte, L. Hyphenated liquid chromatography – gas chromatography technique : Recent evolution and applications. J. Chromatogr. A. 2012; 1255: 100–111.
13.    Sharma, S., Singh, N., Ankalgi, A. D., Rana, A. Singh Ashawat, M. A Laconic Review on Liquid Chromatography Mass Spectrometry (LC-MS) Based Proteomics Technology in Drug Discovery. Asian J. Pharm. Res. 2021; 11: 194–201.
14.    I.D. Wilson, U. A. T. B. Hyphenation and hypernation: The practice and prospects of multiple hyphenation. J. Chromatogr. A. 2003; 1000: 325–356.
15.    Abha, D., Avinash, K., Ganesh, S. Javed, A. Impurity Profiling With Use of Hyphenated Techniques. Asian J. Res. Chem. 2012; 5: 875–881.
16.    Pan, C. et al. The use of LC/MS, GC/ MS, and LC/NMR hyphenated techniques to identify a drug degradation product in pharmaceutical development. J. Phaemaceutical Biomed. Anal. 2019; 40: 581–590.
17.    Sakhreliya, B. D., Kansara, S. LC-NMR : A powerful tool for analyzing and characterizing complex chemical mixtures without the need of chemical separation. J. Pharm. Sci. Biosci. Res. 2013; 3: 115-121.
18.    Hamid Khan, J. A. Identification of Impurities and Degradation Products in Pharmaceutical Products- Role of Hyphenated Techniques. Asian J. Res. Chem. 2017; 7: 31–35.
19.    Rabie S. Farag, S. R. A. A.-S. Confirmatory Method for Determination of 11-Nor- 9 - Tetrahydrocannabinol-9-Carboxylic Acid in Urine Samples Using Gas Chromatography – Mass Spectrometry (GC/MS). Asian J. Res. Chem. 2011; 4: 373–376.
20.    Pallavi S. Thombare, R. N. K. Review on Hyphenated Techniques. World J. Pharm. Res. 2020; 9: 370–377.
21.    Maher, W. et al. Overview of hyphenated techniques using an ICP-MS detector with an emphasis on extraction techniques for measurement of metalloids by HPLC–ICPMS. Microchem. J. 2012; 105: 15–31.
22.    Patel, K. N., Patel, J. K., Patel, M. P., Rajput, G. C. and Patel, H. A. Introduction to hyphenated techniques and their applications in pharmacy. Pharm. Methods. 2010;1: 2–13.
23.    Thamizhanban, D., Rani, T., Pravalika, P. A Review on Hyphenated Separation Techniques Used in Pharmaceutical Analysis. J. Pharm. Biol. Sci. 2016; 11: 65–74.

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