Arshiya Zulfeen Mohd Fahim, Mohammed Shakir Ghouse, Syed Qumarul Islam, Mohammed Abdul Mughni Danish, Shaikh Mehmood, Shaikh Saniya, Quraishi Inshrah Fatima
firstname.lastname@example.org , email@example.com
Ms. Arshiya Zulfeen Mohd Fahim1, Mr. Mohammed Shakir Ghouse2, Mr. Syed Qumarul Islam1, Mr. Mohammed Abdul Mughni Danish1, Mr. Shaikh Mehmood1, Ms. Shaikh Saniya1, Ms. Quraishi Inshrah Fatima1
1Lecturer, Aayan Education and Welfare Trust’s, Aurangabad Pharmacy College, Mitmita, Aurangabad.
2Principal, Aayan Education and Welfare Trust’s, Aurangabad Pharmacy College, Mitmita, Aurangabad.
Volume - 11,
Issue - 2,
Year - 2021
Quality by Design (QbD) is a modern, scientific approach that formalizes product design, automates manual test¬ing, and streamlines troubleshooting. It uses a system¬atic approach to ensure quality by developing a thorough understanding of the compatibility of a finished product to all of the components and processes involved in manufac¬turing that product. Olibanum also known as “Dhup”, Indian Frankincense is an oleo gum resin of Boswellia species. In India it is obtained from Boswellia serrata. Boswellia serrata (Burseraceae) The major use of Boswellia serrata in contemporary medicine is as an anti-arthritic and anti-inflammatory pharmacological agent. The anti-inflammatory properties of the gum resin are attributed to the presence of “boswellic acids” The four major pentacyclic triterpenic acids present in the acidic extract of Boswellia serrata gum resin. ß-Boswellic Acid, Acetyl-ß-Boswellic Acid, 11-keto-ß-Boswellic Acid, Acetyl-11-keto-ß-Boswellic Acid. Oleo gum resin was subjected for solubility studies in different solvents. In this 10 commonly available solvents were used for extraction including water. All of them showed same TLC pattern except water. In case of maceration no heat is employed, but the material has to be extracted multiple times with fresh solvent each time. In this method solvent requirement increases. Each time the extract was checked for presence of boswellic acids by TLC to ensure complete extraction. It took 4-5 times repeated extraction for complete extraction of Boswellic acids by maceration. Literature survey reveals that anti-inflammatory activities associated with this resin are completely restricted to presence of Boswellic acids. So focus in experimental work done is placed on isolation of acid fraction of oleo gum resin. As mentioned in the procedures above acid fraction was obtained as white precipitate. This white precipitate was separated, dried and weighed. Amount of acid fraction obtained was determined on weight basis. In the experimental work done isolation of acid fraction was carried out by procedures mentioned in section above. This is common procedure which uses treatment of resin with alkali to convert acid into its salt and then precipitating salt of acid by using mineral acid. Studies were carried out to check variations in amount of acid portion obtained when parameters were altered. Use of 2% KOH followed by dilute hydrochloric acid as mineral acid will be most suitable.
Cite this article:
Arshiya Zulfeen Mohd Fahim, Mohammed Shakir Ghouse, Syed Qumarul Islam, Mohammed Abdul Mughni Danish, Shaikh Mehmood, Shaikh Saniya, Quraishi Inshrah Fatima. Isolation and Identification of Boswellic Acids by Modern Analytical Techniques. Asian Journal of Pharmaceutical Analysis. 2021; 11(2):98-2. doi: 10.52711/2231-5675.2021.00018
Arshiya Zulfeen Mohd Fahim, Mohammed Shakir Ghouse, Syed Qumarul Islam, Mohammed Abdul Mughni Danish, Shaikh Mehmood, Shaikh Saniya, Quraishi Inshrah Fatima. Isolation and Identification of Boswellic Acids by Modern Analytical Techniques. Asian Journal of Pharmaceutical Analysis. 2021; 11(2):98-2. doi: 10.52711/2231-5675.2021.00018 Available on: https://ajpaonline.com/AbstractView.aspx?PID=2021-11-2-8
1. Rueda DC1, Raith M1, De Mieri M1, Schoffmann A2, Hering S2, Hamburger M3. Identification of dehydroabietc acid from Boswellia thurifera resin as a positive GABAA receptor modulator. Fitoterapia. 2014 Sep 5. pii: S0367-326X (14)00238-X.
2. Khan MA1, Singh M2, Khan MS2, Najmi AK1, Ahmad S2. Caspase Mediated Synergistic Effect of Boswellia serrata Extract in Combination with Doxorubicin against Human Hepatocellular Carcinoma. Biomed Res Int. 2014; 2014: 294143.
3. Kessler CS1, Pinders L, Michalsen A, Cramer H, Ayurvedic interventions for osteoarthritis: a systematic review and meta-analysis, Rheumatol Int. 2014 Jul 26.
4. Jalili C1, Salahshoor MR1, Moradi S2, Pourmotabbed A3, Motaghi M2, The therapeutic effect of the aqueous extract of Boswellia serrata on the learning deficit in kindled rats, Int J Prev Med. 2014 May; 5(5):5 63-8.
5. Thummuri D1, Jeengar MK1, Shrivastava S1, Areti A1, Yerra VG1, Yamjala S1, Komirishetty P1, Naidu VG2, Kumar A1, Sistla R3, Boswellia ovalifoliolata abrogates ROS mediated NF-κB activation, causes apoptosis and chemosensitization in Triple Negative Breast Cancer cells,
6. Dalla Libera D1, Colombo B, Pavan G, Comi G, Complementary and alternative medicine (CAM) use in an Italian cohort of pediatric headache patients: the tip of the iceber Neurol Sci. 2014 May;35 Suppl 1:145-8. doi: 10.1007/s10072-014-1756-y.g,
7. Zaki AA1, Hashish NE2, Amer MA2, Lahloub MF2, Cardioprotective and antioxidant effects of oleogum resin "Olibanum" from Bos Boswellia carteri Birdw. (Bursearceae). Chin J Nat Med. 2014 May;12(5): 345-50. doi: 10.1016/S1875-5364(14)60042-X.
8. Cameron M1, Chrubasik S, Oral herbal therapies for treating osteoarthritis, Cochrane Database Syst Rev. 2014 May 22;5: CD002947. doi: 10.1002/14651858.CD002947.pub2.
9. Verhoff M1, Seitz S, Paul M, Noha SM, Jauch J, Schuster D, Werz O, Tetra- and pentacyclic triterpene acids from the ancient anti-inflammatory remedy frankincense as inhibitors of microsomal prostaglandin E (2) synthase-1, J Nat Prod. 2014 Jun 27;77(6):1445-51. doi: 10.1021/np500198g. Epub 2014 May 20.
10. Wang H1, Zhang C, Wu Y, Ai Y, Lee DY, Dai R, Comparative pharmacokinetic study of two boswellic acids in normal and arthritic rat plasma after oral administration of Boswellia serrata extract or Huo Luo Xiao Ling Dan by LC-MS, Biomed Chromatogr. 2014 May 8. doi: 10.1002/bmc.3182.,
11. Mostafa DM1, Ammar NM, Basha M, Hussein RA, El Awdan S, Awad, Transdermal microemulsions of Boswellia carterii Bird: formulation, characterization and in vivo evaluation of anti-inflammatory activity, Drug Deliv. 2014 Apr 14.
12. Hamidpour R, Hamidpour S, Hamidpour M, Shahlari M, Frankincense (rǔ xiāng; boswellia species): from the selection of traditional applications to the novel phytotherapy for the prevention and treatment of serious diseases,.J Tradit Complement Med. 2013 Oct;3(4):221-6. doi: 10.4103/2225-4110.119723
13. Dey D1, Chaskar S, Athavale N, Chitre D, Inhibition of LPS-Induced TNF-α and NO Production in Mouse Macrophage and Inflammatory Response in Rat Animal Models by a Novel Ayurvedic Formulation, BV-9238, Phytother Res. 2014 Apr 7. doi: 10.1002/ptr.5151. [Epub ahead of print]
14. Ahmed M1, Ali D2, Harrath AH2, Hussain T3, Al-Daghri N3, Alokail MS3, Aladakatti RH4, Ghodesawar MA5, Ultrastructural and hormonal changes in rat cauda epididymal spermatozoa induced by Boswellia papyrifera and Boswellia carterii, C R Biol. 2014 Apr;337(4): 250-7. doi: 10.1016/j.crvi.2014.01.007.
15. Schrott E1, Laufer S2, Lämmerhofer M2, Ammon HP3, Extract from gum resin of Boswellia serrata decreases IA (2)-antibody in a patient with "Late onset Autoimmune Diabetes of the Adult" (LADA), Phytomedicine. 2014 May 15; 21(6): 786. doi: 10.1016/j.phymed.2014.02.004,
16. Umar S1, Umar K2, Sarwar AH3, Khan A4, Ahmad N5, Ahmad S5, Katiyar CK6, Husain SA7, Khan HA8, Boswellia serrata extract attenuates inflammatory mediators and oxidative stress in collagen induced arthritis, Phytomedicine. 2014 May 15;21(6): 847-56. doi: 10.1016/j.phymed.2014.02.001.
17. Wang R1, Wang Y, Gao Z, Qu X, The comparative study of acetyl-11-keto-beta-boswellic acid (AKBA) and aspirin in the prevention of intestinal adenomatous polyposis in APC(Min/+) mice. Drug Discov Ther. 2014 Feb; 8(1): 25-32.,
18. Hartmann RM1, Fillmann HS, Morgan Martins MI, Meurer L, Marroni NP, Boswellia serrata has Beneficial Anti-Inflammatory and Antioxidant Properties in a Model of Experimental Colitis, Phytother Res. 2014 Sep;28(9):1392-8. doi: 10.1002/ptr.5142. Epub 2014 Mar 12.
19. Hosseini-Sharifabad M1, Esfandiari E, Effect of Boswellia serrata gum resin on the morphology of hippocampal CA1 pyramidal cells in aged rat, Anat Sci Int. 2014 Feb.
20. Ahangarpour A, Heidari H1, Fatemeh RA, Pakmehr M, Shahbazian H, Ahmadi I, Mombeini Z, Mehrangiz BH, Effect of Boswellia serrata supplementation on blood lipid, hepatic enzymes and fructosamine levels in type2 diabetic patients, J Diabetes Metab Disord. 2014 Feb 4;13(1):29. doi: 10.1186/2251-6581-13-29.
21. Stürner KH1, Verse N, Yousef S, Martin R, Sospedra M, Boswellic acids reduce Th17 differentiation via blockade of IL-1β-mediated IRAK1 signaling, Eur J Immunol. 2014 Apr;44(4):1200-12. doi: 10.1002/eji.201343629. Epub 2014 Feb 16.,
22. Bagul P1, Khomane KS1, Bansal AK, Investigating permeability related hurdles in oral delivery of 11-keto-β-boswellic acid, Int J Pharm. 2014 Apr 10;464(1-2):104-10. doi: 10.1016/j.ijpharm.2014.01.019. Epub 2014 Jan 22.
23. Nikam TD1, Ghorpade RP2, Nitnaware KM1, Ahire ML1, Lokhande VH1, Chopra A3, Micropropagation and non-steroidal anti-inflammatory and anti-arthritic agent boswellic acid production in callus cultures of Boswellia serrata Roxb, Physiol Mol Biol Plants. 2013 Jan;19(1):105-16. doi: 10.1007/s12298-012-0137-3.
24. Ali SI1, Zhang CR1, Mohamed AA1, El-Baz FK1, Hegazy AK2, Kord MA2, Nair MG3, Major constituents of Boswellia carteri resin exhibit cyclooxygenase enzyme inhibition and antiproliferative activity, Nat Prod Commun. 2013 Oct;8(10):1365-6.
25. Wang YG1, Ren J, Wang AG, Yang JB, Ji TF, Ma QG, Tian J, Su YL, Hepatoprotective prenylaromadendrane-type diterpenes from the gum resin of Boswellia carterii, J Nat Prod. 2013 Nov 22;76(11):2074-9. doi: 10.1021/np400526b. Epub 2013 Nov 6.
26. Moein P1, Abbasi Fard S, Asnaashari A, Baratian H, Barekatain M, Tavakoli N, Moein H, The effect of Boswellia serrata on neurorecovery following diffuse axonal injury, Brain Inj. 2013;27(12):1454-60. doi: 10.3109/02699052.2013.825009. Epub 2013 Oct 2.
27. Rahimi R1, Nikfar S, Abdollahi M, Induction of clinical response and remission of inflammatory bowel disease by use of herbal medicines: a meta-analysis. World J Gastroenterol. 2013 Sep 14;19(34):5738-49. doi: 10.3748/wjg. v19.i34.5738
28. Saxena S1, Uniyal V, Bhatt RP, Inhibitory effect of essential oils against Trichosporon ovoides causing Piedra Hair Infection. Braz J Microbiol. 2012 Oct;43(4):1347-54. doi: 10.1590/S1517-838220120004000016. Epub 2012 Jun 1.