Author(s):
Sourav Duari, Ayan Das, R.N. Pal
Email(s):
souravduari2017@gmail.com
DOI:
10.52711/2231-5675.2025.00012 
Address:
Sourav Duari*, Ayan Das, R.N. Pal
Department of Pharmaceutics, Calcutta Institute of Pharmaceutical Technology and Allied Health Sciences, Maulana Abul Kalam Azad University of Technology, Uluberia, West Bengal, India.
*Corresponding Author
Published In:
Volume - 15,
Issue - 2,
Year - 2025
ABSTRACT:
The study was aimed to create and verify a new, straight-forward, accurate, and specific UV-Spectrophotometric analysis method of Lornoxicam, by comparing in-between three different types of body fluids, having three different pH, like a) gastric fluid, b) physiological fluid c) intestinal fluid, and to determine the best site for administration also. UV spectrophotometric analysis was carried out by utilizing a Shimadzu UV-visible spectrophotometer, using three different mediums for the drug lornoxicam. The standard solution's spectrum was run from 200 to 400 nm range in order to figure out the absorption maximum (?max) of lornoxicam, which was discovered at 353 nanometers. The standard solution’s absorbance of 0.3, 1, 3, 5, 7, 9, 10, 15, and 20 micrograms per milliliter was determined the solution of 353 nm against the blank. Then the graph was plotted by taking concentration on x-axis and absorbance on the y-axis. The same thing was performed for the identification of another two individual solutions in the UV spectrophotometry method. And then they were compared among themselves for suitability. Accordingly, the site of drug administration was determined. The linearity of the UV spectrophotometry method for three fluids were noted. Then it was noticed that the absorption of physiological fluid, having a pH value of 7.4, was found to be the best option for determining the UV spectrophotometry method. The drug lornoxicam was best soluble in pH 7.4 buffer as compared with pH 1.2 and 6.8 buffers. In the suggested UV visible spectrophotometry, ICH criteria were followed, and the findings and statistical parameters showed that the development technique was sensitive, exact, and easy to use for lornoxicam estimation in bulk form.
Cite this article:
Sourav Duari, Ayan Das, R.N. Pal. UV-Spectrophotometric Method Development and Determination of Suitable Medium for Lornoxicam by Comparing In-Between Three Body Fluids. Asian Journal of Pharmaceutical Analysis. 2025; 15(2):75-9. doi: 10.52711/2231-5675.2025.00012
Cite(Electronic):
Sourav Duari, Ayan Das, R.N. Pal. UV-Spectrophotometric Method Development and Determination of Suitable Medium for Lornoxicam by Comparing In-Between Three Body Fluids. Asian Journal of Pharmaceutical Analysis. 2025; 15(2):75-9. doi: 10.52711/2231-5675.2025.00012 Available on: https://ajpaonline.com/AbstractView.aspx?PID=2025-15-2-1
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