Author(s):
Sama Venkatesh, Vijaya Laxmi, Rajeswari Chiluka, Yenumula Padmavathi
Email(s):
rajeswari930@gmail.com
DOI:
10.52711/2231-5675.2022.00036 
Address:
Sama Venkatesh, Vijaya Laxmi, Rajeswari Chiluka, Yenumula Padmavathi
Pillar No 23, G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad.
*Corresponding Author
Published In:
Volume - 12,
Issue - 4,
Year - 2022
ABSTRACT:
Piperine is an alkaloid obtained from Piper nigrum Linn., is known to modify the biotransformation of the drugs by inhibiting Cytochrome P450 forms. Piperine inhibits the bio-transformation and metabolism of metformin leading to higher levels of drug in systemic circulation, thus increases the bioavailabilty when co-administered with metformin. In the present study a simple, accurate, precise, economical procedures, entailing neither irksome sample treatment nor tedious extraction process a combined tablet dosage form of metformin and piperine have been developed for the simultaneous estimation of piperine and metformin using UV- Spectrophotometry method. The developed method was based on employing simultaneous equation method for analysis of both the drugs. Metformin and piperine have shown absorbance maxima at 237nm and 342nm respectively, in methanol and the linearity was obeyed over the concentration range of 2-10µg/ml and 2-10 µg/ml respectively for metformin and piperine. The validation parameters like precision, accuracy, limit of quantitation (LOQ), limit of detection (LOD), were determined according to ICH guidelines. All the results were found to be within the limits.
Cite this article:
Sama Venkatesh, Vijaya Laxmi, Rajeswari Chiluka, Yenumula Padmavathi. Development of UV- Spectrophotometric Method for Simultaneous Estimation of Metformin and Piperine in Combined Tablet Dosage Form. Asian Journal of Pharmaceutical Analysis. 2022; 12(4):221-7. doi: 10.52711/2231-5675.2022.00036
Cite(Electronic):
Sama Venkatesh, Vijaya Laxmi, Rajeswari Chiluka, Yenumula Padmavathi. Development of UV- Spectrophotometric Method for Simultaneous Estimation of Metformin and Piperine in Combined Tablet Dosage Form. Asian Journal of Pharmaceutical Analysis. 2022; 12(4):221-7. doi: 10.52711/2231-5675.2022.00036 Available on: https://ajpaonline.com/AbstractView.aspx?PID=2022-12-4-1
REFERENCES:
1. Rang HP, Dale MM, Ritter JM, Flower RJ, Henderson G: Pharmacology. 7th edition. Spain: Elsevier Churchil Livingstone; 2012.
2. Tripathi KD: Essentials of Medical Pharmacology. 6th edition. Jaypee Brothers Medica Publisher Pvt Ltd; 2013.
3. Trease and evan’s Pharmacogonosy 14th edition; Paras Medical Book Publishers; 363-364.
4. Indian Pharmacopoeia, Indian Pharmacopoeial Comission, Ghaziabad, 2014, volume II, 3244-3255.
5. Umang H, Abkita H. Uv spectrophotometric and rp- hplc methods for simultaneous estimation of isoniazid, rifampicin and piperine in pharmaceutical dosage form. Internaional Journal of Pharmacy and Pharmaceutical Sciences. 2015; 6(10):274-280.
6. Kaushik G. Kapuriya, Prakash M, Parmar, Hiral R, Topiya. Method development and validation of rifampicine and piperine in their combined dosage form by UV spectrophotometric and RP-HPLC methods. International Bulletin of Drug Research. 2015;1(2): 71-80.
7. Jaldip Jasoliya, Aashka Jani. Method development and validation of RP-HPLC method
1. for simultaneous estimation of resveratroland piperine in combined capsule dosage form. World Journal of Pharmacy and Pharmaceutical Sciences. 2014; 3(5):1096-1107.
8. Vipul Upadhyay, Neeru Sharma, Himanshu M, Joshi, Amreesh Malik, Manoj, Mishra, B.P Singh, Sanjeev Tripathi. Development and validation of rapid RP-HPLC Method for estimation of piperine in Piper nigrum. National Journal of Herbal Medicine 2013; 1 (4): 6-9.
9. Sejal Patel, Niraj Vyas.Validated spectrofluorimetric method for estimation of piperine in an Ayurvedic formulation. Asian Journal of Pharmaceutical and Clinical Research. 2012; 5(4):231.245.
10. Jenil C Khamar, Satish A, Patel J. Q-Absorbance Ratio Spectrophotometric Method for The Simultaneous Estimation of Rifampicin and Piperine in their Combined Capsule Dosage. Journal of Applied Pharmaceutical Science 02 (04); 2012: 137-141.
11. Beckett AH, Stenlake JB. Practical Pharmaceutical Chemistry: Ultraviolet-visible absorption spectrophotometric; 2002.
12. International Conference on Harmonization, Q2 (R1): Validation of Analytical Procedures; Text and Methodology. 1997; 62 (96): 27463-67.
13. Krishna Veni Nagappan, Meyyanathan S N, Rajinikanth B Raja, Elango Kannan. A Liquid Chromatography Method for the Simultaneous Determination of Curcumin and Piperine in Food Products Using Diode Array Detection. Asian J. Research Chem. 2(2): April-June, 2009 page 115-118.
14. Tripti Jain, Kamlesh Dashora. Spectrophotometric fingerprinting method for Unani formulation Hab-e- Azarakhi. Asian J. Pharm. Tech. 2(1): Jan.-Mar. 2012; Page 01-03. 12. Beckett AH, Stenlake JB. Practical Pharmaceutical Chemistry: Ultraviolet-visible Absorption Spectrophotometric; 2002.
15. International Conference on Harmonization, Q2 (R1): Validation of Analytical Procedures; Text and Methodology. 1997; 62 (96): 27463-67.
16. Hiral J. Varachhiya, Rohan K. Barse, Suresh Jain. Development and Validation of Spectroscopic Simultaneous Equation Method for Simultaneous Estimation of Itopride Hydrochloride and Omeprazole in Synthetic Mixture. Asian J. Pharm. Res. 2019; 9(4):238-242.
17. Audumbar Mali, Ankush Hake, Manoj Patil, Ritesh Bathe, Ashpak Tamboli. Simultaneous UV Spectrophotometric Methods for Estimation of Paracetamol and Domperidone in Bulk and Tablet Dosage Form. Asian J. Res. Pharm. Sci. 6(1): Jan.-Mar., 2016; Page 21-26.
18. Kalpana Vasanthan, R Vijayageetha, A Shantha Arcot. Method Development and Validation of RP-HPLC Method for Estimation of Nateglinide in Bulk Drug and Pharmaceutical Formulation. Research J. Pharm. and Tech.3 (3): July-Sept. 2010; Page 804-806.
19. B. Siddartha, I. Sudheer Babu, C. Parthiban, C. Madhavi. Development and Validation of UV–Spectrophotometric Method of Pantoprazole in bulk and Pharmaceutical dosage form. Research J. Pharma. Dosage Forms and Tech. 2013; 5(6): 341-344
20. Sonali D. Rathod, P.M. Patil, S. B. Jadhav, P.D. Chaudhari. UV Spectrophotometric Simultaneous Determination of Metformine Hydrochloride and Pioglitazone Hydrochloride in Combined Dosage Form. Asian J. Pharm. Ana. 2(1): Jan.-Mar. 2012; Page 05-09.
21. Swapnil D. Jadhav, Akshay A. Magdum, Ramchandra M. Panchal, Pradip S. Koli. Simultaneous Estimation of Metformin Hydrochloride and Rosiglitazone Maleate from Tablet Dosage Form by Derivative Spectroscopic Method. Asian J. Pharm. Ana. 3(3): July-Sept. 2013; Page 94-97.
22. Sushil D. Patil, Sayali K. Chaure, Sanjay Kshirsagar. Development and validation of UV spectrophotometric method for Simultaneous estimation of Empagliflozin and Metformin hydrochloride in bulk drugs. Asian J. Pharm. Ana. 2017; 7(2): 117-123.