Author(s): Shiny Ganji, D. Satyavati

Email(s): gshiny.d@gmail.com

DOI: 10.5958/2231-5675.2015.00001.0   

Address: Shiny Ganji1*, Dr. D. Satyavati2
1St Anns College of Pharmacy, Nayunipally (V), Vetapalem, Chirala, Prakasam (Dt) 523187 Andhra Pradesh
2Sri Duttha Institute of Pharmacy, Sheriguda, Ibrahimpatnam, R.R. Dist. Andhra Pradesh
*Corresponding Author

Published In:   Volume - 5,      Issue - 1,     Year - 2015


ABSTRACT:
The prime aim of the current work is to develop and validate a novel, specific, sensitive, precise, rapid and faster isocratic elution, RP HPLC method for estimation of Cobicistat and related impurities in bulk and pharmaceutical dosage forms. Chromatographic separation was achieved on Inertsil ODS – 3V column (250 mm X 4.6 mm, 5µ) using an isoratic mode with mobile phase composed of potassium dihydrogen phosphate buffer (PH 2.5) and acetonitrile in the ratio of 30:70 v/v. The flow rate was 1.0ml/min, temperature is maintained at ambient and detection was made at 240 nm. The run time was 15 min. The developed method was validated according to the ICH guide lines and different analytical parameters such as linearity, precision, accuracy, specificity, limit of detection, limit of quantitation were determined. The linearity of calibration curve for each analyte in concentration range of 400 µg/ml -1200µg/ml. is good. There exists good correlation between peak area and analyte concentration. Relative standard deviation values for cobicistat is 0.099 and impurity is 0.6636. LOD for drug and impurity is 0.02 % and 0.20 % respectively. LOQ for drug and impurity is 0.06 % and 0.60% respectively. Hence the proposed method is highly sensitive, precise, accurate, robust and fast. The short retention time allows the analysis of large number of samples in short period of time and it is cost effective, so it can be successfully applied for routine analysis of active pharmaceutical ingredient and related impurities in bulk and pharmaceutical dosage forms.


Cite this article:
Shiny Ganji, D. Satyavati. Development and validation of RP-HPLC method for the analysis of Cobicistat and related impurities in bulk and pharmaceutical dosage forms. Asian J. Pharm. Ana. 5(1): Jan.- March 2015; Page 1-8. doi: 10.5958/2231-5675.2015.00001.0


Recomonded Articles:

Author(s): Sushil D. Patil, Sunil V. Amurutkar, C.D. Upasani

DOI: 10.5958/2231-5675.2016.00030.2         Access: Open Access Read More

Author(s): Kirthi A, Shanmugam R, Mohana Lakshmi S , Ashok Kumar CK, Padmini K, Shanti Prathyusha M, Shilpa V

DOI:         Access: Open Access Read More

Author(s): Hamid Khan, Javed Ali

DOI: 10.5958/2231-5675.2017.00020.5         Access: Open Access Read More

Author(s): G. Krishnamoorthy, C. Diana Priyadarshini, R. Senthamarai

DOI:         Access: Open Access Read More

Author(s): Pinaz A. Kasad, K.S. Muralikrishna

DOI:         Access: Open Access Read More

Author(s): P. Janaki Pathi, N. Appala Raju, G.Parvathalu

DOI:         Access: Open Access Read More

Author(s): Paras Virani, Rajanit Sojitra, Hasumati Raj, Vineet Jain

DOI: 10.5958/2231-5675.2015.00025.3         Access: Open Access Read More

Author(s): Khushboo Patel*, Dhara Patel, Jaya Dwivedi, Priyanka Newal, Dhananjay Meshram

DOI: 10.5958/2231-5675.2017.00039.4         Access: Open Access Read More

Author(s): B. Thangabalan, M. Salomi, N. Sunitha, S. Manohar Babu

DOI:         Access: Open Access Read More

Author(s): Mansi J. Chaudhari, Suraj R. Chaudhari, Shailesh S. Chalikwar, Atul A. Shirkhedkar

DOI: 10.5958/2231-5675.2018.00008.X         Access: Open Access Read More

Author(s): Saroj Kumar Raul, Bukkuru Spandana, Patibandla Sameera, Vegiraju Vikitha

DOI:         Access: Open Access Read More

Author(s): S. Vidyadhara, RLC Sasidhar, B. Venkateswara Rao, P. Ratna Kumari

DOI:         Access: Open Access Read More

Author(s): Shobha Rani G, Lohita M, Jaya Preethi P, Madhavi R, Sunisitha B, Mounika D

DOI:         Access: Open Access Read More

Author(s): Madhavi R., Mohana Krishna A., Shobha Rani G., Mounika D.

DOI: 10.5958/2231-5675.2015.00008.3         Access: Open Access Read More

Asian Journal of Pharmaceutical Analysis (AJPA) is an international, peer-reviewed journal, devoted to pharmaceutical analysis...... Read more >>>

RNI: Not Available                     
DOI: 10.5958/2231–5675 

Popular Articles


Recent Articles




Tags