Author(s): Harish A. Gavit, Amol R. Pawar, Vikas V. Patil, Pankaj S. Patil, Pushpendra H. Sisodiya


DOI: 10.52711/2231-5675.2024.00017   

Address: Harish A. Gavit1*, Amol R. Pawar1,2, Vikas V. Patil1, Pankaj S. Patil1, Pushpendra H. Sisodiya1
1Department of Quality Assurance, Kisan Vidya Prasarak Sanstha's, Institute of Pharmaceutical Education, Boradi 425405.
2Research Scholar, Sankalch and Patel University, Visnagar - 384315 (Gujarat - India)
*Corresponding Author

Published In:   Volume - 14,      Issue - 2,     Year - 2024

Today, chromatography is the backbone of separation science and is being used in all research laboratories and pharmaceutical industries of the world. In these chromatography techniques, HPLC is one of the chromatographic techniques, which is mostly used analytical technique. Recent developments in chromatographic supports and instrumentation for liquid chromatography (LC) are enabling rapid and highly efficient separations. This article represents a brief review of HPLC along with its principle and instrumentation. It describes about new trends in HPLC such as RRLC, UPLC, UFLC and Nano LC. In this article mainly focus on detailed comparison of new developments of HPLC in terms of instrumental operating conditions, applications and advantages over HPLC of each technique. Keywords: Liquid chromatography, HPLC, RRLC, UPLC, UFLC, Nano LC.

Cite this article:
Harish A. Gavit, Amol R. Pawar, Vikas V. Patil, Pankaj S. Patil, Pushpendra H. Sisodiya. Advancement in High-Performance Liquid Chromatography Techniques: A Comprehensive Review. Asian Journal of Pharmaceutical Analysis. 2024; 14(2):95-3. doi: 10.52711/2231-5675.2024.00017

Harish A. Gavit, Amol R. Pawar, Vikas V. Patil, Pankaj S. Patil, Pushpendra H. Sisodiya. Advancement in High-Performance Liquid Chromatography Techniques: A Comprehensive Review. Asian Journal of Pharmaceutical Analysis. 2024; 14(2):95-3. doi: 10.52711/2231-5675.2024.00017   Available on:

1.    Ettre L S. M.S. Tswett and The Invention of Chromatography, LC-GC., 2003; 21: 458467.
2.    Narayudu Yandamuri. Comparative Study of New Trends in HPLC: A Review
3.    MukthiThammana. A review on HPLC. 2016:5:22
4.    Klaus Unger. Introduction of High Performance Liquid Chromatography. 6-7
5.    Gita Chawla and Krishna Kr. Chaudhary. A review of HPLC technique covering its pharmaceutical, environmental, forensic, clinical and other applications. International Journal of Pharmaceutical Chemistry and Analysis. 2019; 6(2): 27-39
6.    Satheeshkumar; M. Gokul; S. Shalini; M. Ukeshraj and M. Mullaivendan. HPLC method development and validation – A review. International Journal of Advance Research, Ideas and Innovations in Technology. 7(3).
7.    Abdu Hussen Ali. High-Performance Liquid Chromatography (HPLC): A review. Ann Adv Chem. 2022; 6: 010- 020.
8.    Heewon Lee. Pharmaceutical applications of liquid chromatography coupled with mass spectrometry (LC/MS). Journal of Liquid Chromatography and Related Technologies. 2005; 28: 7-8.
9.    Salgado P, Visnevschi-Necrasov T, Kiene R.P, Azevedo I, Rocha A.C.S, Almeida C.M.R, Magalhaes C. Determination of 3-mercaptopropionic acid by HPLC: A sensitive method for environmental applications, Journal of Chromatography B, 2015; 992: 103-108.
10.    Ma Bokai, Gou Xin Lei, Zhao Xin Ying. Application of high-performance liquid chromatography in food and drug safety analysis. Journal of Food Safety and Quality. 2016; 7: 4295- 4298.
11.    Ian M Bird. High-Performance Liquid Chromatography: Principles and Clinical Applications. Br Med J. 1989; 299: 783-7.
12.    Ante M. Krstulovic. Investigation of catecholamine metabolism using high-performance liquid chromatography: Analytical methodology and clinical applications. Journal of Chromatography B: Biomedical Sciences and Applications. 1982;229: 1-34.
13.    K. Takatera and T. Watanabe. Anal. Chem. 1993; 65: 3644.
14.    J. Szpunar. Trends Anal. Chem., 2000; 19: 127
15.    Yandamuri, N.; Nagabattula, K.R.S.; Kurra, S.S.; Batthula, S.; Allada, L.P.S.N.; Bandam. A Review: HPLC method development and validation. Int. J. Anal. Bioanal. Chem. 2015; 5(4): 76-81.
16.    Rao, B.V.; Sowjanya, G.N.; Ajitha, A.; Rao, V.U.M. Review on stability indicating HPLC method development. World J. Pharm. Pharm. Sci. 2015; 4(8): 405-423.
17. webassets/ cms/ category/ media/ other_i mages/ primer_e_lcsystem.jpg
18.    Yoshida T, Ronald E.M and Kumagai H. High−Speed Analyses using Rapid Resolution Liquid Chromatography on ZORBAX column packed 1.8 µm Particles. Chromatography. 2007; 28: 81-87.
19.    Dr. M ichael E. Swartz and Brian J. Murphy. Ultra performance liquid chromatography: Tomorrow’s HPLC technology today, Novel Phases for HPLC, as published in LPI- June 2004 URL: uploads/ tx_ttproducts/ datasheet/ ultra-performance-liquid-chromatographytomorrow's-hplc-technology-today.pdf
20.    M eenakshi D, Meenaxi M. A Review on Ultra Performance Liquid Chromatography. International Journal of Drug Development and Research. 2013; 5?: 29-34
21.    Ashok Kumar, Gautam S, Anroop N and Rishbha S, UPLC: A Preem inent technique in Pharmaceutical Analysis, Acta Poloniae Pharmaceutica Drug Research. 2012; 69: 371-380.
22.    Swartz M. E. and M urphy B., Pharm. Formulation Quality. 2004; 6: 40.
23.    Ultra-fast liquid chromatography, URL:.http/ /
24. about/ pressrelease/ oh80jt000000 abel.html
25.    He Qing., Integrated nano liquid chromatography system on a chip., Ph.D.Thesis, California Institute of Technology, Pasadena, California, USA, June 21, 2005.
26.    http:/ nano-hplc/ nano-lcdefinition
27.    Shuijun L, Gangyi L, Jingying J, Xiaochuan L, Chen Y (2006) J Pharm Biomed Anal 40: 987-992
28.    Vishwanathan K, Bartlett MG, Steward JT. Determination of gatifloxian in human plasma by liquid chromatography/ electrospray tandem mass spectroscopy. Rapid Commun Mass Spectrom. 2001; 15: 915-919.
29.    Song HH, Choi KS, Kim CW, Kwon YE. Pharmacokinetic Profiles of Two Branded Formulations of Piroxicam 20mg in Healthy Korean Volunteers by a Rapid Isocratic HPLC Method. J BioequivAvailab. 2009; 1: 074-081.
30.    Ni Y, Liu Y, Kokot S. Two-dimensional fingerprinting approach for comparison of complex substances analysed by HPLC-UV and fluorescence detection. Analyst. 2011; 136:550-559
31.    Elbarbry FA, Mabrouk MM, El-Dway MA. Determination of the analgesic components of Spasmomigraine tablet by liquid chromatography with ultraviolet detection. J AOAC Int. 2007; 90: 94-101a
32.    Hinz B, Chevts J, Renner B, Wuttke H, Rau T, et al. Bioavailability of diclofenac potassium at low doses. Br J Clin Pharmacol. 2007; 59: 80-84
33.    G Subramanian, P Musmade, S Agarwal, N Udupa. Simultaneous RP HPLC estimation of tinidazole, diclofenac potassium and paracetamol in tablets. Indian Journal of Pharmaceutical Sciences. 2004; 66: 694-696a.
34.    Barde PS, Desai AY, Roy MNS, Vaidya VV. Simultaneous RP HPLC determination of Camylofindihydrochloride in Pharmaceutical preparation. TSI Journal. 2008; 7(10)a.
35.    Singh RR, Rathnam MV, Vegesna R (2008) Simultaneous RP HPLC determination of Camylofindihydrochloride and Paracetamol in Pharmaceutical preparations.TSI Journal 7(11).
36.    Sethi PD.Identification of Drugs in Pharmaceutical Formulations by Thin Layer Chromatography. CBS Publishers, New Delhi. 1992
37.    Ashok kumar S, Senthil Raja M, Perumal P. RP-HPLC Method Development and Validation for Simultaneous Estimation of Montelukast Sodium and levocetirizineDihydrochloride. International Journal of Pharmaceutical Research. 2009; 1: 8-12.
38.    Smita Sharma MC, Sharma DV, Kohlib AD, Sharma C. Development and validation of tlc-densitometry method for simultaneous quantification of montelukast sodium and levocetirizinedihydrochloride pharmaceutical solid dosage form. Der Pharmacia Lettre. 2010; 2: 489-494.
39.    Rathnam MV, Singh RR. Simultaneous RP HPLC Determination of CamylofinDihydrochloride and Diclofenac Potassium in Pharmaceutical Preparations. Pharm Anal Acta. 2010; 1:108.
40.    Natesan S, Thanasekaran D, Krishnaswami V, Ponnusamy C. Improved RP- HPLC Method for the Simultaneous Estimation of Tranexamic Acid and Mefenamic Acid in Tablet Dosage Form. Pharm Anal Acta.  2011; 2:115.
41.    Subbaiah PR, Kumudhavalli MV, Saravanan C, Kumar M, Chandira RM. Method Development and Validation for estimation of MoxifloxacinHCl in tablet dosage form by RP-HPLC method. Pharm Anal Acta. 2010; 1:109.
42.    Dharmender R, Madhavi T, Reena A, Sheetal A. Simultaneous Quantification of Bergenin, (+)-Catechin, Gallicin and Gallic acid; and quantification of β-Sitosterol using HPTLC from Bergeniaciliata (Haw.) Sternb. Forma ligulata Yeo (Pasanbhe da). Pharm Anal Acta. 2010; 1: 104.
43.    Sonawane LV, Bari SB. Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Amoxicillin Trihydrate and Bromhexine Hydrochloride from Oily Suspension. Pharm Anal Acta. 2010; 1:107.
44.    Prabu SL, Srinivasan M, Thiagarajan S, Marina Q. Simultaneous Determination of Gatifloxacin and Ambroxol Hydrochloride in a Tablet Formulation by Liquid Chromatography. Pharm Anal Acta. 2010; 1:110.
45.    Lacassie E, Gaulier JM, Marquet P, Rabatel JF, Lachâtre G. Methods for the determination of seven selective serotonin reuptake inhibitors and three active metabolites in human serum using high-performance liquid chromatography and gas chromatography. Journal of Chromatography B. 2000; 742: 229-238a
46.    Sharma A, Conway WD, Staudinger RM. Reverse-phase highperformance liquid chromatographic determination of taxol in mouse plasma. J Chromatogr. 1994; 655: 315-319.
47.    Arjanova OV, Prihoda ND, Yurchenko LV, Sokolenko NI, Frolov VM, et al. Phase 2 Trial V-5 Immunitor (V5) in Patients with Chronic Hepatitis- C Co-infected with HIV and Mycobacterium tuberculosis. J Vaccines Vaccin. 2010; 1: 103.
48.    Nannan Panday VR, MeerumTerwot JM, Ten BokkelHuinink WW. The role of pro drug therapy in the treatment of cancer. Proc Am SocClinOncol. 2010; 17: 742a
49.    Rezaei, Z., andAsadollahi-Baboli, M. RP-HPLC method development and validation for the determination of four major bioactive flavonoids in onion (Allium cepa) extract. Analytical Chemistry Letters. 2021; 11(1): 78-92.
50.    S. Saha, S. Bera, P. Banerjee, S. Bose, and A. B. Chatterjee, Recent advances in the development and application of monolithic stationary phases for reversed-phase liquid chromatography. TrAC Trends Anal. Chem. 2020; 123: 115769, 2020.
51.    M. I. Alam, N. N. Chowdhury, and M. Z. Rahman. Recent advances in the development of reversed-phase high-performance liquid chromatography methods for the analysis of small molecules.  J. Chromatogr. A. 2020; 1626: 461298.
52.    K. Ma, Y. Xiong, Y. Liang, and X. Qiao. Recent advances in the development of chiral stationary phases for reversed-phase liquid chromatography. J. Sep. Sci. 2021; 44(1); 55-72.
53.    B. W. Armstrong. Recent advances in chiral stationary phases for reversed-phase high-performance liquid chromatography. J. Chromatogr. A. 2017; 1511: 113-125.
54.    J. Wang, Y. Luo, J. Hu, J. Cheng, and H. Chen. Recent advances in the application of deep eutectic solvents in reversed-phase liquid chromatography.  J. Chromatogr. A. 2021; 1643: 462028.

Recomonded Articles:

Author(s): Sushil D. Patil, Sunil V. Amurutkar, C.D. Upasani

DOI: 10.5958/2231-5675.2016.00030.2         Access: Open Access Read More

Author(s): Dipti G. Phadtare, Pawar Amol R, R.B. Saudagar

DOI: 10.5958/2231-5675.2016.00019.3         Access: Closed Access Read More

Author(s): Pankaj Bhamare, Rupal Dubey, Neeraj Upmanyu, Pothuvan Umadoss

DOI: 10.5958/2231-5675.2021.00001.6         Access: Open Access Read More

Author(s): Hamid Khan, Javed Ali

DOI: 10.5958/2231-5675.2017.00020.5         Access: Open Access Read More

Author(s): G. Krishnamoorthy, C. Diana Priyadarshini, R. Senthamarai

DOI:         Access: Open Access Read More

Author(s): Shobha Rani G, Lohita M, Jaya Preethi P, Madhavi R, Sunisitha B, Mounika D

DOI:         Access: Open Access Read More

Author(s): Pinaz A. Kasad, K.S. Muralikrishna

DOI:         Access: Open Access Read More

Author(s): Kirthi A, Shanmugam R, Mohana Lakshmi S , Ashok Kumar CK, Padmini K, Shanti Prathyusha M, Shilpa V

DOI:         Access: Open Access Read More

Author(s): Mansi J. Chaudhari, Suraj R. Chaudhari, Shailesh S. Chalikwar, Atul A. Shirkhedkar

DOI: 10.5958/2231-5675.2018.00008.X         Access: Open Access Read More

Author(s): Sri Lakshmi D, Jane T Jacob, Srinivasa Sastry D, Satyanarayana D

DOI: 10.5958/2231-5675.2017.00005.9         Access: Open Access Read More

Asian Journal of Pharmaceutical Analysis (AJPA) is an international, peer-reviewed journal, devoted to pharmaceutical analysis...... Read more >>>

RNI: Not Available                     
DOI: 10.5958/2231–5675 

Recent Articles