UV-Spectrophotometric method has been developed and validated for quantitative estimation of dasatinib in bulk and pharmaceutical formulation. Dasatinib is soluble in acetonitrile, so it was used as solvent. Dasatinib was dissolved in acetonitrile and resulting solution was scanned in UV range (200-400nm). The ?max was found to be 315nm. Beers law is valid in concentration range of 5-25µg/ml. The developed method was validated for linearity, accuracy, precision, robustness; LOD and LOQ. Linearity was obtained in the range of 5-25µg/ml with correlation coefficient 0.9992. LOD and LOQ were found to be 0.908µg/ml and 2.752µg/ml respectively. The method showed good reproducibility and recovery so; proposed method can be applied for routine analysis of dasatinib in bulk and pharmaceutical formulation.
Cite this article:
Jyoti Mittha, Bhavana Habib. UV Spectrophotometric Method Development and Validation of Dasatinib in Bulk and Formulation. Asian Journal of Pharmaceutical Analysis. 2021; 11(3):203-6. doi: 10.52711/2231-5675.2021.00036
Jyoti Mittha, Bhavana Habib. UV Spectrophotometric Method Development and Validation of Dasatinib in Bulk and Formulation. Asian Journal of Pharmaceutical Analysis. 2021; 11(3):203-6. doi: 10.52711/2231-5675.2021.00036 Available on: https://ajpaonline.com/AbstractView.aspx?PID=2021-11-3-6
1. Arti M, Sangeetha G. Formulation and evaluation of Immediate relase film coated tablets of an anti cancer drug (Dasatinib). Research Journal of Pharmacy and Technology. 2019; 12(2): 729-734.
2. Dasatinib [Internet] Drug bank [cited 2021 Feb 19 ]. Available from: https://go.drugbank.com/drugs/DB01254
3. Saili et al. UV Spectrophometric Method Development and Validaton ofDasatinib in Bulk and Solid Dosage Form. International Journal of Current Pharmaceutical Research. 2020; 12(4): 90-93.
4. Panchumarthy R, Anusha S, Srinivasa B. Development and validation of UV spectrophotometric method for determination of Dasatinib in bulk and pharmaceutical dosage form and its degradation behavior under various stress conditions. International Journal of Pharmaceutical Sciences Review and Research. 2018; 53(2): 45-50.
5. Natraj et al. Method development and validation for estimation of dasatinib monohydrate tablets by RP-HPLC. Asian Journal of Research in Chemistry. 2013; 6(9): 859-862.
6. Madhuri et al. Method development and validation of stability indicating RP-HPLC method for estimation of Dasatinib in tablet dosage form. Journal of Pharmacy Research. 2017; 11(5): 419-423.
7. Bandi R, Naidu N. Validation of RP-HPLC method for estimation of Dasatinib in bulk and its pharmaceutical dosage form. International Journal of Pharmacy and Biological Sciences. 2014; 4(1): 61-68.
8. Vatchavai et al. Development and Validation of new RP-HPLC method for the Estimation of Dasatinib in pharmaceutical dosage form. Asian Journal of Pharmaceutical Technology and Innovation. 2017; 5(23): 7-12.
9. Rao et al. Analytical method development and validation of Dasatinib in its pharmaceutical dosage form by UPLC with forced degradation studies. International Journal for Pharmaceutical Research Scholars. 2013; 2(4): 221-227.
10. Bhole R, Bonde C, Biradar P. Development and validation of stability indicating HPTLC method for estimation of Dasatinib and characterization of degradation products by using MS. Eurasian Journal of Analytical Chemistry. 2018; 13(4): 1-11.
11. ICH Q2 (R1). Validation of Analytical Procedures: Text and Methodology. ICH Harmonized Tripartite Guidelines, 1994.
12. Santosh et al. UV- Spectrophotometric development and validation of Zidovudine tablet dosage form. Asian Journal of Pharmaceutical Analysis. 2018; 8(4): 195-202.