Author(s): Govinda Rao Kamala, Sowjanya Vadrevu, Malipeddi Haripriya

Email(s): govindarao83@gmail.com

DOI: 10.5958/2231-5675.2015.00031.9   

Address: Govinda Rao Kamala*, Sowjanya Vadrevu, Malipeddi Haripriya
Aditya Pharmacy College, ADB Road, Surampalem, Kakinada, East Godavari District
*Corresponding Author

Published In:   Volume - 5,      Issue - 4,     Year - 2015


ABSTRACT:
The developed RP- HPLC method allows rapid and precise determinations of Omeprazole and domperidone, the scope of the present work is to expand and optimization of the chromatographic conditions, to develop RP-HPLC method. A series of mobile phases were tried, among the various mobile phases as methanol and 0.1% ammonium acetate an ideal mobile phase, since it gave a good resolution and peak shapes with perfect optimization. The flow rate was optimized at 1 ml/min. The Linearity and Correlation Coefficient of Omeprazole and Domperidone were found to be 10-50 ug/ml, and 10.10-50.50 ug/ml 0.9996, and 0.998 respectively. The limit of detection for Omeprazole and domperidone was found to be 1.76 and 2.0 and the limit of quantification was found to be 1.87 and 1.48. The method was known to be accurate with the assay method. The % assay was found to be 97 and 98.12. The developed method was showed to a good accuracy and precision. The % RSD is for Omeprazole and Domperidone is 041 and 1.41. The Isocratic elution technique developed for the determination of Omeprazole and domperidone ideally suited for rapid and routine analysis. This method shows that good reproducibility of the results. Furthermore this method was simple, sensitive, and accurate. Degradation studies were done, here the drug stability results were in the range of acceptance criteria 85-115%.


Cite this article:
Govinda Rao Kamala, Sowjanya Vadrevu, Malipeddi Haripriya. Method Development and Validation for Simultaneous Estimation of Omeprazole and Domperidone by RP-HPLC. Asian J. Pharm. Ana. 5(4): 2015; 195-205. doi: 10.5958/2231-5675.2015.00031.9


Recomonded Articles:

Author(s): Sushil D. Patil, Sunil V. Amurutkar, C.D. Upasani

DOI: 10.5958/2231-5675.2016.00030.2         Access: Open Access Read More

Author(s): K. Pramod, Shahid H. Ansari, Javed Ali

DOI:         Access: Open Access Read More

Author(s): Kirthi A, Shanmugam R, Mohana Lakshmi S , Ashok Kumar CK, Padmini K, Shanti Prathyusha M, Shilpa V

DOI:         Access: Open Access Read More

Author(s): Sushil D. Patil, Sayali K. Chaure, Maswood Ahmed Hafizur Rahman, Prajkta U. Varpe, Sanjay Kshirsagar.

DOI: 10.5958/2231-5675.2017.00004.7         Access: Open Access Read More

Author(s): Hamid Khan, Javed Ali

DOI: 10.5958/2231-5675.2017.00020.5         Access: Open Access Read More

Author(s): Pinaz A. Kasad, K.S. Muralikrishna

DOI:         Access: Open Access Read More

Author(s): P. Janaki Pathi, N. Appala Raju, G.Parvathalu

DOI:         Access: Open Access Read More

Author(s): Paras Virani, Rajanit Sojitra, Hasumati Raj, Vineet Jain

DOI: 10.5958/2231-5675.2015.00025.3         Access: Open Access Read More

Author(s): B. Thangabalan, M. Salomi, N. Sunitha, S. Manohar Babu

DOI:         Access: Open Access Read More

Author(s): Saroj Kumar Raul, Bukkuru Spandana, Patibandla Sameera, Vegiraju Vikitha

DOI:         Access: Open Access Read More

Author(s): S. Vidyadhara, RLC Sasidhar, B. Venkateswara Rao, P. Ratna Kumari

DOI:         Access: Open Access Read More

Author(s): Hamid Khan, Mushir Ali, Alka Ahuja, Javed Ali

DOI:         Access: Open Access Read More

Author(s): Jayshree Pawar, Sandeep Sonawane, Santosh Chhajed, Sanjay Kshirsagar

DOI: 10.5958/2231-5675.2016.00024.7         Access: Open Access Read More

Author(s): Md. Ahsanul Haque, Mohammad Shahriar, Most. Nazma Parvin, S. M. Ashraful Islam

DOI:         Access: Open Access Read More

Asian Journal of Pharmaceutical Analysis (AJPA) is an international, peer-reviewed journal, devoted to pharmaceutical analysis...... Read more >>>

RNI: Not Available                     
DOI: 10.5958/2231–5675 

Popular Articles


Recent Articles




Tags