ABSTRACT:
In present study, on a Jasco V-730 spectrophotometer, the method for estimating acetazolamide was used, with ethanol: distilled water (5:5) serving as the solvent. For acetazolamide, the recovery analysis research indicates recoveries of 102.55%, 102.68%, and 103.35% for various concentrations of the spiked medication. For quantifying acetazolamide in bulk and tablet dosage form, an RP-HPLC method was created and validated with mobile phase acetonitrile: water (80:20). For acetazolamide, the assay (% w/w) was found 98.6 %, 98.5 %, 98.9 %. The flow rate was 1ml/min. The sample volume was 20.00µl. Run time 5 min and retention time was 2.044.
Cite this article:
S. P. Chougule. Development of Analytical method by UV and HPLC of Acetazolamide in bulk and tablet dosage form. Asian Journal of Pharmaceutical Analysis. 2024; 14(2):60-4. doi: 10.52711/2231-5675.2024.00011
Cite(Electronic):
S. P. Chougule. Development of Analytical method by UV and HPLC of Acetazolamide in bulk and tablet dosage form. Asian Journal of Pharmaceutical Analysis. 2024; 14(2):60-4. doi: 10.52711/2231-5675.2024.00011 Available on: https://ajpaonline.com/AbstractView.aspx?PID=2024-14-2-2
REFERENCES:
1. Ravisankar P, Gowthami S, Rao GD. A review on analytical method development. Indian Journal of Research in Pharmacy and Biotechnology. 2014 May 1; 2(3): 1183.
2. Reddy PS, Dayakar M, Swathi P, Srinivasulu D, Reddy NV, Akhila G et al. Method Development and Validation of Acetazolamide by Using UV Spectroscopy. International Journal of Engineering Science and Computing. 2020; 10(6): 26146.
3. Indian Pharmacopoeia, Volume-II 2014, Pg No: 985
4. https://en.wikipedia.org/wiki/Acetazolamide
5. Kumar A, Kishore L, Kaur N, Nair A. Method development and validation: Skills and tricks. Chronicles of Young Scientists. 2012 Jan 1; 3(1): 4.
6. Vidushi Y, Meenakshi B, Bharkatiya MB. A review on HPLC method development and validation. Res J Life Sci, Bioinform, Pharm Chem Sci. 2017; 2(6): 178.
7. Shinde M, Kumar S, Mallik A, Jyothi N. A review on HPLC method development and validation. Epra International Journal of Research and Development (IJRD). 2021 Oct 9; 6(10): 1.
8. Yiasmin MN, Waleed AA. Recent applications of HPLC in food analysis: A mini. 2021 May 7(5).
9. D. D. Masne, K. P. Jatte, M. A. Khachane, R. D. Chakole, M. S. Chard. Degradation Profiling by RP- HPLC: A Review. International Journal of Pharmacy and Pharmaceutical Research. 2021 Jun 21(3): 273-298.
10. Sah P, Chasta MP, Sharma G, Kishore K. High Performance Liquid Chromatography (HPLC). Aug 2021; 8(9): 23-28.
11. Chikanbanjar N, Semwal NU, Jyakhwa U. A review article on analytical method validation. J. Pharm. Innov. 2020; 1: 48-58.
12. Matteo Campanelli. Analytical Method Development and Validation Overview. May 2020.
13. Ghanjaoui ME, Mandil A, Mou AA, Slimani R. High performance liquid chromatography quality control. International Journal of Advanced Chemistry. 2020 Jan; 8(1): 1-1.