Author(s): M.C. Purohit, Anuj Kandwal, Reena Purohit, A.R. Semwal, Shama Parveen, Arun K. Khajuria

Email(s): arun.khajuria20@gmail.com

DOI: 10.52711/2231-5675.2021.00047   

Address: M.C. Purohit1, Anuj Kandwal1, Reena Purohit2, A.R. Semwal3, Shama Parveen4, Arun K. Khajuria5*
1Department of Chemistry, Hemvati Nandan Bahuguna Garhwal University, BGR Campus Pauri, (Garhwal) 246001, Uttarakhand, India.
2Department of Chemistry, Bal Ganga Maha Vidhyalaya, Sendul Kemar, Tehri Garhwal. Uttarakhand, India.
3Department of Chemistry, D.A.V. PG College, Dehradun, Uttarakhand, India.
4Department of Chemistry, Faculty of Science, Motherhood University, District- Haridwar, 247661 Uttrakhand India.
5Department of Botany, Cluster University of Jammu, 180001 Jammu and Kashmir, India.
*Corresponding Author

Published In:   Volume - 11,      Issue - 4,     Year - 2021


ABSTRACT:
Nanoscience and nanotechnology has attracted a lot of attention because of its wide variety of applications. Plant based metallic nanoparticles revolutionized the health sector with targeting nano drug to cure different ailments. Living beings are known to be susceptible to microbial attack followed by multidrug resistance of microorganism put the necessitates for searching more efficient methods of drug delivery or drug production. In the present study, we report the green synthesis of stable hexagonally shaped zinc oxide nanoparticles from leaf extract of Ajuga bracteosa and their antimicrobial efficacy against the selected bacterial (Streptococcus pneumonia, Staphylococcus aureus, Klebsiella pneumonia, Escherichia coli and Pseudomonas aeruginosa) and fungal (Aspergillus fumigates and Trichoderma viride) strains by using agar well diffusion method. Initial colour change and surface-plasmon-resonance (SPR) absorbance bands between 349 nm gave support to the synthesis of zinc oxide nanoparticles. These nanoparticles were further characterized by XRD, EDX, TEM and FTIR techniques. XRD analysis showed that nanoparticles are crystalline in nature. TEM measurements showed that nanoparticles are hexagonally shaped with their average size less than 27 nm. FTIR spectra confirms the presence of phytochemicals which were responsible for reducing, capping and stabilizing the nanoparticles. Antimicrobial results of the synthesized ZnO nanoparticles has indicated the good potential of nanoparticles against all tested microorganism in the present study.


Cite this article:
M.C. Purohit, Anuj Kandwal, Reena Purohit, A.R. Semwal, Shama Parveen, Arun K. Khajuria. Antimicrobial Activity of Synthesized Zinc Oxide Nanoparticles using Ajuga bracteosa Leaf Extract. Asian Journal of Pharmaceutical Analysis. 2021; 11(4):275-0. doi: 10.52711/2231-5675.2021.00047

Cite(Electronic):
M.C. Purohit, Anuj Kandwal, Reena Purohit, A.R. Semwal, Shama Parveen, Arun K. Khajuria. Antimicrobial Activity of Synthesized Zinc Oxide Nanoparticles using Ajuga bracteosa Leaf Extract. Asian Journal of Pharmaceutical Analysis. 2021; 11(4):275-0. doi: 10.52711/2231-5675.2021.00047   Available on: https://ajpaonline.com/AbstractView.aspx?PID=2021-11-4-5


REFERENCES:
1.    Ahmad L, Semotiuk AJ, Liu QR, Rashid W, Mazari P, Rahim K, Sadia S. Anti-hypertensive plants of rural Pakistan: Current use and future potential. Journal of Complementary Medicine Research. 7(2); 2018:138-153.
2.    Anbuvannan M, Ramesh M, Viruthagiri G, Shanmugam N, Kannadasan N. Anisochilus carnosus leaf extract mediated synthesis of zinc oxide nanoparticles for antibacterial and photocatalytic activities. Materials Science in Semiconductor Processing. 39; 2015:621-628.
3.    Azizi S, Mohamad R, and Mahdavi SM. Green microwave-assisted combustion synthesis of zinc oxide nanoparticles with Citrulluscolocynthis (L.) Schrad: Characterization and Biomedical Applications. Molecules, 22(2); 2017: 1-13.
4.    Azizi S, Mohamad R, Bahadoran A, Bayat S, Rahim RA, Ariff A, Saad WZ. Effect of annealing temperature on antimicrobial and structural properties of bio-synthesized zinc oxide nanoparticles using flower extract of Anchusa italica. Journal of Photochemistry and Photobiology B: Biology. 161; 2016:441-449.
5.    Babu EP, Subastri A, Suyavaran A, Rao PL, Kumar MS, Jeevaratnam K, Thirunavukkarasu C. Extracellularly synthesized ZnO nanoparticles interact with DNA and augment gamma radiation induced DNA damage through reactive oxygen species. RSC Advances. 5(76); 2015:62067-62077.
6.    Collett H. (1980). Flora Simlensis: a handbook of the flowering plants of Simla and neighbourhood, 2nd Impression, Calcutta and Simla, Thacker, Spink and Co, 407.
7.    Elumalai K, Velmurugan S. Green synthesis, characterization and antimicrobial activities of zinc oxide nanoparticles from the leaf extract of Azadirachta indica (L.). Applied Surface Science. 345; 2015: 329-36.
8.    Fu L and Fu Z. Plectranthusamboinicus leaf extract–assisted biosynthesis of ZnO nanoparticles and their photocatalytic activity. Ceramics International, 41(2); 2015: 2492-2496.
9.    Ganaie HA, Ali MN, Ganai BA, Bashir S. Antimutagenic activity of compounds isolated from Ajuga bracteosa Wall ex. Benth against EMS induced mutagenicity in mice. Toxicology Reports. 5; 2018:108-112.
10.    Gaur RD. (1999). Flora of the District Garhwal, North West, 1st edition, Transmedia, 459.
11.    Gondwal M and Pant G. Synthesis and catalytic and biological activities of silver and copper nanoparticles using Cassia occidentalis. International Journal of Biomaterials, 2018: 1-10.
12.    Gulzar S, Nawchoo IA, Yaqoob U, Hassan AA. A study on variation of phytochemical constituents with altitude and in vitro antimicrobial potential of two species of Ajuga L. Advances in Biomedicine and Pharmacy. 4(1); 2017:19-30.
13.    Hamayun M, Khan A, Afzal S, Khan MA. Study on traditional knowledge and utility of medicinal herbs of district Buner, NWFP, Pakistan. Indian Journal of Traditional Knowledge, 5(3); 2006: 407-412.
14.    Hussain J, Begum N, Hussain H, Khan FU, Rehman NU, Al-Harrasi A, Ali L. Ajuganane: a new phenolic compound from Ajuga bracteosa. Natural Product Communications, 7(5), 615-616.
15.    Iravani S, Korbekandi H, Mirmohammadi SV and Zolfaghari B. Synthesis of silver nanoparticles: chemical, physical and biological methods. Research in Pharmaceutical Sciences. 9(6); 2014: 385-406.
16.    Jasim NO. Antifungal activity of Zinc oxide nanoparticles on Aspergillusfumigatus fungus and Candida albicans yeast. Journal of Naural Sciences Research, 5; 2015: 23-27.
17.    Kaithwas G, Gautam R, Jachak SM, Saklani A. Antiarthritic effects of Ajuga bracteosa Wall ex Benth. in acute and chronic models of arthritis in albino rats. Asian Pacific Journal of Tropical Biomedicine. 2(3); 2012:185-188.
18.    Kalyani RL, Pammi S V N, Kumar P V, Swamy P V, and Murthy KR. Antibiotic potentiation and anti-cancer competence through bio-mediated ZnO nanoparticles. Materials Science and Engineering: C, 103; 2019: 1-11.
19.    Kandwal A, Purohit MC, Khajuria AK, Joshi RK. Green Synthesis, Characterization and Antimicrobial Activity of Silver Nanoparticles Using Leaf Extract of Ajuga Parviflora Benth. In Wall. Plant Archives. 19(2); 2019:762-768.
20.    Kandwal A, Purohit P, Khajuria AK, Kumar G, Purohit MC and Mandal NK. Synthesis of zinc oxide nanoparticles using leaf extract of Ajuga parviflora Benth. In Wall and their characterization. Journal of Pharmacognosy and Phytochemistry. 8; 2019b: 493-96.
21.    Kayani WK, Dilshad E, Ahmed T, Ismail H, Mirza B. Evaluation of Ajuga bracteosa for antioxidant, anti-inflammatory, analgesic, antidepressant and anticoagulant activities. BMC Complementary and Alternative Medicine. 16(1); 2016:1-13.
22.    Khajuria AK, Bisht NS, Manhas RK, Kumar G. Callus mediated biosynthesis and antibacterial activities of zinc oxide nanoparticles from Viola canescens: An important Himalayan Medicinal Herb. SN Applied Sciences. 1(5); 2019:1-13.
23.    Khajuria AK, Kumari M, Kandwal A, Singh A, Bisht NS. Biofabrication of Zinc Oxide Nanoparticles from Two Different Zinc Sources and Their Antimicrobial Activity. BioNanoScience. 2020:1-7.
24.    Khajuria AK, Negi A, Bisht NS, Maurya V, Kandwal A. Green Synthesis, Characterization and Antimicrobial Activity of Zinc Oxide Nanoparticles Using Root Extract of Viola canescensWall. ex. Roxb. Asian Journal of Chemistry, 31; 2019b: 551-554.
25.    Kouvaris P, Delimitis A, Zaspalis V, Papadopoulos D, Tsipas SA and Michailidis N. Green synthesis and characterization of silver nanoparticles produced using Arbutus unedo leaf extract. Materials Letters, 76; 2012: 18-20.
26.    Kumar S, Javed MS, Kumar PKR, and Kumar R. Chemical Composition, in vitro Antibacterial, And Antioxidant Activity of Essential Oil from Leaves of Ajuga Parviflora Benth. Asian Journal of Pharmaceutical and Clinical Research, 11(2); 2018: 57-61.
27.    Kumar S, Raj H, and Sharma J. Ethnobotanical explorations in the Balh valley region of North Western Himalaya. Methodology, 2(7); 2013: 40-44.
28.    Pal SL, Jana U, Manna PK, Mohanta GP, and Manavalan R. Nanoparticle: An overview of preparation and characterization. Journal of Applied Pharmaceutical Science, 1(6); 2011: 228-234.
29.    Pala A, Jadona M, Katarea YK, Singoura PK, Rajakb H, Chaurasiyaa PK, Patila UK, Pawar RS. Ajuga bracteosa wall: a review on its ethnopharmacological and phytochemical studies. Der Pharmacia Sinica. 2(2); 2011:1-10.
30.    Parveen S, Sharma G, Sharma SB. and Kandwal A. A Review on Synthesis and Biological Evaluation of Plants Based Metallic Nanoparticles. Global Journal of Research and Review. 8; 2021: 1-17.
31.    Purohit MC, Kandwal A, Khajuria AK, Singh M, Rawat R. Antimicrobial Activity of Ajuga Parviflora Leaf Extract Mediated Synthesized Zinc Oxide Nanoparticles. Plant Archives, 20(2); 2020: 1627-30.
32.    Qing X, Yan HM, Ni ZY, Vavricka CJ, Zhang ML, Shi QW, Gu YC, Kiyota H. Chemical and pharmacological research on the plants from genus Ajuga. Heterocyclic Communications. 23(4); 2017:245-268.
33.    Rahman N, Ahmad M, Riaz M, Mehjabeen JN, Ahmad R. Phytochemical, antimicrobial, insecticidal and brine shrimp lethality bioassay of the crude methanolic extract of Ajuga parviflora Benth. Pakistan Journal of Pharmaceutical Sciences. 26(4); 2013:751-756.
34.    Rajiv P, Rajeshwari S, Venckatesh R. Bio-Fabrication of zinc oxide nanoparticles using leaf extract of Parthenium hysterophorus L. and its size-dependent antifungal activity against plant fungal pathogens. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 112; 2013:384-387.
35.    Riaz N, Malik A, Nawaz SA, Muhammad P, Choudhary MI. Cholinesterase‐Inhibiting Withanolides from Ajuga bracteosa. Chemistry and Biodiversity. 1(9); 2014:1289-1295.
36.    Sangeetha G, Rajeshwari S, Venckatesh R. Green synthesis of zinc oxide nanoparticles by aloe barbadensis miller leaf extract: Structure and optical properties. Materials Research Bulletin. 46(12); 2011:2560-2566.
37.    Santhoshkumar J, Kumar SV, Rajeshkumar S. Synthesis of zinc oxide nanoparticles using plant leaf extract against urinary tract infection pathogen. Resource-Efficient Technologies. 3(4); 2017:459-465.
38.    Senthilkumar SR and Sivakumar T. Green tea (Camellia sinensis) mediated synthesis of zinc oxide (ZnO) nanoparticles and studies on their antimicrobial activities. International Journal of Pharmacy and Pharmaceutical Sciences, 6(6); 2014: 461-465.
39.    Setyawati MI, Tay CY, Leong DT. Effect of zinc oxide nanomaterials-induced oxidative stress on the p53 pathway. Biomaterials. 34(38); 2013:10133-42.
40.    Shad AA, Zeeshan M, Fazal H, Shah HU, Ahmed S, Abeer H, Abd_Allah EF, Ullah R, Afridi H, Asma MA. Physio-chemical evaluation and biological activity of Ajuga bracteosa wall and Viola odoroto Linn. African Journal of Traditional, Complementary and Alternative Medicines. 13(2); 2016:40-46.
41.    Shah RK, Boruah F, and Parween N. Synthesis and characterization of ZnO nanoparticles using leaf extract of Camelliasinesis and evaluation of their antimicrobial efficacy. International Journal of Current Microbiology and Applied Science, 4(8); 2015: 444-450.
42.    Sharma D, Rajput J, Kaith BS, Kaur M, Sharma S. Synthesis of ZnO nanoparticles and study of their antibacterial and antifungal properties. Thin Solid Films.;519(3); 2010:1224-1229.
43.    Sharmila G, Thirumarimurugan M, Muthukumaran C. Green synthesis of ZnO nanoparticles using Tecoma castanifolia leaf extract: characterization and evaluation of its antioxidant, bactericidal and anticancer activities. Microchemical Journal. 145; 2019:578-87.
44.    Shekhawat MS, Ravindran CP, Manokari M. Biosynthesis of zinc oxide nanoparticles from Passiflora foetida L. extracts and their characterization. International Journal of Green and Herbal Chemistry. 3(2); 2014:518-523.
45.    Stoimenov PK, Klinger RL, Marchin GL, Klabunde KJ. Metal Oxide Nanoparticles as Bactericidal Agents. Langmuir, 18; 2002: 6679-86.
46.    Supraja N, Prasad TN, Krishna TG, David E. Synthesis, characterization, and evaluation of the antimicrobial efficacy of Boswellia ovalifoliolata stem bark-extract-mediated zinc oxide nanoparticles. Applied Nanoscience. 6(4); 2016:581-590.
47.    Verma, V., Mahmood, U., and Singh, B. (2002). Clerodanediterpenoids from Ajuga bracteosa Wall. Natural Product Letters, 16(4); 2012: 255-259.
48.    Vidya C, Hiremath S, Chandraprabha MN, Antonyraj ML, Gopal IV, Jain A, Bansal K. Green synthesis of ZnO nanoparticles by Calotropis gigantea. International Journal of Current Engineering and Technology. 1(1); 2013:118-20.
49.    Vohra A and Kaur H. Chemical investigation of medicinal plant Ajugabracteosa. Journal of Natural Product and Plant Resource, 1(1); 2011: 37-45.
50.    Xie Y, He Y, Irwin PL, T Jin and X. Shi. Antibacterial Activity and Mechanism of Action of Zinc Oxide Nanoparticles against Campylobacter jejuni. Applied and Environmental Microbiology, 77; 2011: 2325-31.
51.    Yousaf T, Rafique S, Wahid F, Rehman S, Nazir A, Rafique J, Aslam K, Shabir G, Shah SM. Phytochemical profiling and antiviral activity of Ajuga bracteosa, Ajuga parviflora, Berberis lycium and Citrus lemon against Hepatitis C Virus. Microbial Pathogenesis. 118; 2018:154-158.

Recomonded Articles:

Author(s): S.G. Killedar, K.I. Kope, S.B. Sangle , M.S. Tamboli

DOI:         Access: Open Access Read More

Author(s): AK. Prabhakaran, P. Kumaravel, J. Priya, G. Melchias, A. Edward, G. Sridevi

DOI:         Access: Open Access Read More

Author(s): M.C. Purohit, Anuj Kandwal, Reena Purohit, A.R. Semwal, Shama Parveen, Arun K. Khajuria

DOI: 10.52711/2231-5675.2021.00047         Access: Closed Access Read More

Asian Journal of Pharmaceutical Analysis (AJPA) is an international, peer-reviewed journal, devoted to pharmaceutical analysis...... Read more >>>

RNI: Not Available                     
DOI: 10.5958/2231–5675 

Popular Articles


Recent Articles




Tags