Vinod Matole, Yogesh Thorat, Akshay Javalgikar, Shraddha Jamakhandi, Pradip Pawar
Vinod Matole*, Dr. Yogesh Thorat, Akshay Javalgikar, Shraddha Jamakhandi, Pradip Pawar
D.S.T.S. Mandal’s College of Pharmacy, Solapur-413004, Maharashtra, India.
Volume - 11,
Issue - 1,
Year - 2021
The analytical method was developed and validated for determination of acyclovir in Tablet by High performance liquid chromatography. The separation was carried out on Luna C18 column (150*4.6mm, 5µm). The mobile phase consists of methanol: water in the ratio 50:50 at flow rate 1ml/min with diode array detector wavelength at 248nm. The column temperature was adjusted at 38º ± 0.8?C with injection volume 20µl. The retention time of acyclovir was 2.718min. The linearity of the calibration curve was linear over the concentration range 40-60µg/ml (r2=1). The validation was carried out as per ICH guidelines. The development method was easy, rapid, linear, precise, accurate and consistent.
Cite this article:
Vinod Matole, Yogesh Thorat, Akshay Javalgikar, Shraddha Jamakhandi, Pradip Pawar. Development and Validation of RP-HPLC Method for the Estimation of Acyclovir in API and Tablet Formulation. Asian J. Pharm. Ana. 2021; 11(1):41-44. doi: 10.5958/2231-5675.2021.00008.9
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