Author(s): Sridevi Ranjitha Karanam, V. Reena Jyothi Swarupa

Email(s): ranjitha.karanam@gmail.com

DOI: 10.5958/2231-5675.2016.00002.8   

Address: Sridevi Ranjitha Karanam1*, V. Reena Jyothi Swarupa2
1Assistant Professor, Department of Pharmaceutical Analysis, Aditya College of Pharmacy, ADB Road, Aditya Nagar, Surampalem, East Godavari, Andhra Pradesh-533437
2Department of Pharmaceutical Analysis, Aditya College of Pharmacy, ADB Road, Aditya Nagar, Surampalem, East Godavari, Andhra Pradesh-533437
*Corresponding Author

Published In:   Volume - 6,      Issue - 1,     Year - 2016


ABSTRACT:
A simple, sensitive, precise and specific Reverse Phase High Performance Liquid Chromatographic method was developed and validated for the determination of Valsartan and Hydrochlorothiazide in bulk and tablet dosage form. It was found that the excipient in the tablet dosage form does not interfere in the quantification of active drug by proposed method. The HPLC separation was carried out by reverse phase chromatography by Hypersil BDS C-18 (150 x 4.6 mm) column with a mobile phase composed of water: Acetinitrile: OPA in the ratio of 95:5:1(M.P-A) and Acetinitrile: water: OPA in the ratio of 95:5:1(MP-B) in Gradient mode at a flow Rate of 1.5ml/min. The detection was monitored at 225nm. The calibration curve for valsartan and HCTZ was linear from 50-300 µg/ml and 3.7-22.5 µg/ml respectively. The proposed method has adequate sensitivity, reproducibility and specificity for the determination of Valsartan and HCTZ.


Cite this article:
Sridevi Ranjitha Karanam, V. Reena Jyothi Swarupa. Stability Indicating Analytical Method Development and Validation for Simultaneous Estimation of Valsartan and Hydrochlorothiazide in Tablet Dosage Form. Asian J. Pharm. Ana. 6(1): January- March, 2016; Page 7-14. doi: 10.5958/2231-5675.2016.00002.8


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DOI: 10.5958/2231–5675 

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