Author(s): Revathi R., Venkata Naga Suresh P., Koteswara Rao M., Ethiraj T., Rajarajan S.

Email(s): revathethiraj@gmail.com

DOI: Not Available

Address: Revathi R.*, Venkata Naga Suresh P.1, Koteswara Rao M.1, Ethiraj T.2, Rajarajan S.3
1Dept. of Pharmaceutical Analysis, 2 Dept. of Pharmaceutics, 3 Dept. of Pharmacy Practice, The Erode College of Pharmacy and Research, Erode-638112, Tamil Nadu.
*Corresponding Author

Published In:   Volume - 2,      Issue - 4,     Year - 2012


ABSTRACT:
Objective of the present work is to optimize condition to develop UV zero order, first derivative spectrophotometric method and a simple, rapid and reproducible RP-HPLC method for the estimation of didanosine in selected dosage form. Chromatographic separation of didanosine was achieved with chromosil column (150 mm x 4.6 mm i.d, 5µ particle size), mobile phase used was phosphate buffer: acetonitrile: methanol in the ratio of 20:35:45 with 0.6 ml/min flow rate. The chromatograms were recorded at 248nm.The retention time was < 3 minutes (2.72±0.1). The method was validated for system suitability, precision, accuracy, linearity and robustness. The isocratic LC method offers simplicity, selectivity, precision, accuracy and less time consuming. Hence these two methods can be used for the routine analysis of the estimation of didanosine in Pharmaceutical dosage form.


Cite this article:
Revathi R., Venkata Naga Suresh P., Koteswara Rao M., Ethiraj T., Rajarajan S. Development and Validation of RP-HPLC Method for Content Analysis of Didanosine in Dosage Form. Asian J. Pharm. Ana. 2(4): Oct. - Dec. 2012; Page 118-121.


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