Author(s): Ajay Bedadurge, Kadare Mahesh, Vinod Matole, Parikshit Shirure, Sainath Suryawanshi, Ghodake Kajal

Email(s): matole7414@gmail.com

DOI: 10.52711/2231-5675.2021.00035   

Address: Ajay Bedadurge, Kadare Mahesh, Vinod Matole*, Parikshit Shirure, Sainath Suryawanshi, Ghodake Kajal
Shivai Charitable Trusts College of Pharmacy, Koregaonwadi, Omerga.
*Corresponding Author

Published In:   Volume - 11,      Issue - 3,     Year - 2021


ABSTRACT:
The analytical method was developed and validated for determination of acyclovir in ointment by High performance liquid chromatography. The separation was carried out on Luna C18 column (250 × 4.6mm × 5µ). The mobile phase consists of water: acetonitrile in the ratio 88:12 at flow rate 0.8ml/min with diode array detector wavelength at 254nm.The column temperature was adjusted at 30ºC±40ºC with injection volume 20µl.The retention time of acyclovir was 4.747min. The linearity of the calibration curve was linear over the concentration range 80-120µg/ml (r2=0.9996). The validation was carried out as per ICH guidelines. The development method was easy, rapid, linear, precise, accurate and consistent.


Cite this article:
Ajay Bedadurge, Kadare Mahesh, Vinod Matole, Parikshit Shirure, Sainath Suryawanshi, Ghodake Kajal. Development and Validation of RP-HPLC Method for Determation of Acyclovir in Ointment. Asian Journal of Pharmaceutical Analysis. 2021; 11(3):199-2. doi: 10.52711/2231-5675.2021.00035

Cite(Electronic):
Ajay Bedadurge, Kadare Mahesh, Vinod Matole, Parikshit Shirure, Sainath Suryawanshi, Ghodake Kajal. Development and Validation of RP-HPLC Method for Determation of Acyclovir in Ointment. Asian Journal of Pharmaceutical Analysis. 2021; 11(3):199-2. doi: 10.52711/2231-5675.2021.00035   Available on: https://ajpaonline.com/AbstractView.aspx?PID=2021-11-3-5


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