Author(s):
Ajay Bedadurge, Kadare Mahesh, Vinod Matole, Parikshit Shirure, Sainath Suryawanshi, Ghodake Kajal
Email(s):
matole7414@gmail.com
DOI:
10.52711/2231-5675.2021.00035 
Address:
Ajay Bedadurge, Kadare Mahesh, Vinod Matole*, Parikshit Shirure, Sainath Suryawanshi, Ghodake Kajal
Shivai Charitable Trusts College of Pharmacy, Koregaonwadi, Omerga.
*Corresponding Author
Published In:
Volume - 11,
Issue - 3,
Year - 2021
ABSTRACT:
The analytical method was developed and validated for determination of acyclovir in ointment by High performance liquid chromatography. The separation was carried out on Luna C18 column (250 × 4.6mm × 5µ). The mobile phase consists of water: acetonitrile in the ratio 88:12 at flow rate 0.8ml/min with diode array detector wavelength at 254nm.The column temperature was adjusted at 30ºC±40ºC with injection volume 20µl.The retention time of acyclovir was 4.747min. The linearity of the calibration curve was linear over the concentration range 80-120µg/ml (r2=0.9996). The validation was carried out as per ICH guidelines. The development method was easy, rapid, linear, precise, accurate and consistent.
Cite this article:
Ajay Bedadurge, Kadare Mahesh, Vinod Matole, Parikshit Shirure, Sainath Suryawanshi, Ghodake Kajal. Development and Validation of RP-HPLC Method for Determation of Acyclovir in Ointment. Asian Journal of Pharmaceutical Analysis. 2021; 11(3):199-2. doi: 10.52711/2231-5675.2021.00035
Cite(Electronic):
Ajay Bedadurge, Kadare Mahesh, Vinod Matole, Parikshit Shirure, Sainath Suryawanshi, Ghodake Kajal. Development and Validation of RP-HPLC Method for Determation of Acyclovir in Ointment. Asian Journal of Pharmaceutical Analysis. 2021; 11(3):199-2. doi: 10.52711/2231-5675.2021.00035 Available on: https://ajpaonline.com/AbstractView.aspx?PID=2021-11-3-5
REFERENCES:
1. Indian Pharmacopoeia. The Indian Pharmacopoeia Commission 2007: 67-70.
2. Yadav PK, Chandanam S, Rao ST, Akkamma HG, Bharadwajan RG, Deepa MS. New analytical method development and validation of acyclovir by RP-HPLC. Unique J Pharmaceutical and Biological Sciences 2016; 4: 20-26.
3. Williams DA, Lemke TL, editors. Foye’s Principles of Medicinal Chemistry. 7th edition Philadelphia: Lippincott Williams and Wilkins, Baltimore: Wolters and Kluwer Business 2013: 1283.
4. Umesh SD, Satyam ZC, Shweta A, Jadhav and Vaibhav RP. Spectrophotometric determination and validation of acyclovir in tablet dosage form. International J Parma tech research 2012; 4(4): 1840-1845.
5. Bhagyashri PP, Sugandha VM. UV spectrophotometric estimation of acyclovir in bulk and tablet dosage form using area under curve method. World J Pharmacy and Pharmaceutical Sciences 2014; 3(8): 1644-1651.
6. Somshubhra G, Anirban SD, Satyaprabha Harani JA. Method development and validation of acyclovir in tablet form by RP-HPLC Int. J. Pharm. Sci. 2014; 4(3); 1234-1238.
7. Hayam ML et al. Stability indicating methods for the determination of acyclovir in the presence of its degradation product. Analytical Chem. an Indian J. 2010; 9(4): 398-407.
8. Ravishankar PA, Niharika S, Sireesha O, Sai K, Himaja V. Development and validation of RP-HPLC method for quantitative estimation of acyclovir in bulk drug and tablets. J Chemical and Pharmaceutical Sciences 2015; 08(01): 73-80.
9. Vilas C. and Milind UA. Validated stability indicating method HPLC assay method for acyclovir in bulk drug. International J Analytical, Pharmaceutical and Biomedical Science 2012; 23: 01-12.
10. Suchita VG, Varsha M, Jadhav and Vilasrao JK. High pressure liquid chromatographic method development and validation for estimation of acyclovir in bulk and marketed formulation. International J. of Pharmaceutical Sciences and Research 2016; 7(5): 2194-2199.