Author(s): K. Vijaya Sri, S. Deepthi, M. Madhuri, P. V. Aishwarya

Email(s): vijayasree_2002@yahoo.co.in

DOI: 10.5958/2231-5675.2019.00026.7   

Address: K. Vijaya Sri*, S. Deepthi, M. Madhuri, P. V. Aishwarya
Department of Pharmaceutical Analysis, Malla Reddy College of Pharmacy, (Affiliated to Osmania University) Maisammaguda, Secunderabad-500 100
*Corresponding Author

Published In:   Volume - 9,      Issue - 3,     Year - 2019


ABSTRACT:
The main objectives of presented work were developed and validate the UV-spectrophotometric by Q-absorption ratio and RP-HPLC method for the simultaneous estimation of Atazanavir and Ritonavir as per ICH guidelines in bulk and pharmaceutical dosage form. In UV-spectrophotometric, RP-HPLC method was developed by using Acetonitrile used as a solvent and Acetonitrile/ acetate buffer in the ratio of 60:40(%v/v) used as a mobile phase. The RP-HPLC method was developed in Eclipse C18 column (100 mm × 4.6 mm, 3.5 µm particle size). In UV-spectrophotometric method, the ?max of Atazanavir and Ritonavir was 250 and 239.4nm (iso absorption point) and for HPLC –UV detection at 250 nm were found to be respectively. It was proved that linearity in the concentration range of 5–35 µg/ml for Atazanavir, 10-60 µg/ml for Ritonavir and for HPLC the linearity range between 3-150µg/ml for Atazanavir and Ritonavir 1-50 µg/ml with a correlation coefficient value of 0.999. The % RSD for intraday precision and interday precision was less than 2% for UV-spectrophotometric and RP-HPLC method. A new, simple, accurate UV-spectrophotometric and RP-HPLC method were developed for the simultaneous estimation of Atazanavir and Ritonavir in the bulk and in the pharmaceutical dosage form.


Cite this article:
K. Vijaya Sri, S. Deepthi, M. Madhuri, P. V. Aishwarya. Development and Validation of UV Spectroscopic by Q-absorption Ratio, RP-HPLC Method for Simultaneous Estimation of Atazanvir and Ritonavir in bulk and Pharmaceutical Dosage Form. Asian J. Pharm. Ana. 2019; 9(3):138-150. doi: 10.5958/2231-5675.2019.00026.7


Recomonded Articles:

Author(s): Kirthi A, Shanmugam R, Mohana Lakshmi S , Ashok Kumar CK, Padmini K, Shanti Prathyusha M, Shilpa V

DOI:         Access: Open Access Read More

Author(s): G. Krishnamoorthy, C. Diana Priyadarshini, R. Senthamarai

DOI:         Access: Open Access Read More

Author(s): Hamid Khan, Javed Ali

DOI: 10.5958/2231-5675.2017.00020.5         Access: Open Access Read More

Author(s): P. Janaki Pathi, N. Appala Raju, G.Parvathalu

DOI:         Access: Open Access Read More

Author(s): Paras Virani, Rajanit Sojitra, Hasumati Raj, Vineet Jain

DOI: 10.5958/2231-5675.2015.00025.3         Access: Open Access Read More

Author(s): B. Thangabalan, M. Salomi, N. Sunitha, S. Manohar Babu

DOI:         Access: Open Access Read More

Author(s): Khushboo Patel*, Dhara Patel, Jaya Dwivedi, Priyanka Newal, Dhananjay Meshram

DOI: 10.5958/2231-5675.2017.00039.4         Access: Open Access Read More

Author(s): Mansi J. Chaudhari, Suraj R. Chaudhari, Shailesh S. Chalikwar, Atul A. Shirkhedkar

DOI: 10.5958/2231-5675.2018.00008.X         Access: Open Access Read More

Author(s): Saroj Kumar Raul, Bukkuru Spandana, Patibandla Sameera, Vegiraju Vikitha

DOI:         Access: Open Access Read More

Author(s): S. Vidyadhara, RLC Sasidhar, B. Venkateswara Rao, P. Ratna Kumari

DOI:         Access: Open Access Read More

Author(s): Shobha Rani G, Lohita M, Jaya Preethi P, Madhavi R, Sunisitha B, Mounika D

DOI:         Access: Open Access Read More

Author(s): Madhavi R., Mohana Krishna A., Shobha Rani G., Mounika D.

DOI: 10.5958/2231-5675.2015.00008.3         Access: Open Access Read More

Asian Journal of Pharmaceutical Analysis (AJPA) is an international, peer-reviewed journal, devoted to pharmaceutical analysis...... Read more >>>

RNI: Not Available                     
DOI: 10.5958/2231–5675 

Popular Articles


Recent Articles




Tags