Author(s):
M. Venkata Ramana, Podishetty Akhila, Salla Pujitha, Akula Ganesh, Yerolla Soundarya
Email(s):
Email ID Not Available
DOI:
10.52711/2231-5675.2026.00012 
Address:
M. Venkata Ramana1*, Podishetty Akhila2, Salla Pujitha3, Akula Ganesh4, Yerolla Soundarya5
1Principal, Department of Chemistry, Surabhi Dayakar Rao College of Pharmacy, JNTUH, Telangana, India.
2Student, Department of Pharmaceutical Analysis, Surabhi Dayakar Rao College of Pharmacy, JNTUH, Telangana, India.
3Assistant Professor, Department of Pharmacology, Surabhi Dayakar Rao College of Pharmacy, JNTUH, Telangana, India.
4Associate Professor, Department of Chemistry, Surabhi Dayakar Rao College of Pharmacy, JNTUH, Telangana, India.
5Assistant Professor, Department of Pharmaceutical Analysis, Surabhi Dayakar Rao College of Pharmacy, JNTUH, Telangana, India.
*Corresponding Author
Published In:
Volume - 16,
Issue - 2,
Year - 2026
ABSTRACT:
A simple, rapid and reliable reverse phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for the simultaneous estimation of Budesonide and Formetrol in both pure drug substance and tablet dosage forms. Chromatographic separation was achieved using X-Bridge C18 column (4.6 × 150 mm, 5 µm) maintained at ambient temperature, with a mobile phase consisting of Methanol and Water in the ratio of 35:65 (v/v). The flow rate was set at 1.0 mL/min, and the detection was carried out using a UV detector at 235 nm. A 10 µL injection volume and a total run time of 8 minutes allowed efficient and rapid separation of both analytes. This developed method exhibited good resolution, satisfactory peak symmetry and high reproducibility. The parameters were evaluated according to ICH guidelines, the parameters such as linearity, accuracy, precision, specificity, robustness and system suitability. The validated results were found to be within acceptable limits. The validated RP-HPLC method is suitable for quality control analysis of Budesonide & Formoterol in pharmaceutical dosage forms.
Cite this article:
M. Venkata Ramana, Podishetty Akhila, Salla Pujitha, Akula Ganesh, Yerolla Soundarya. Reverse Phase High Performance Liquid Chromatography Method for Simultaneous Estimation of Budesonide and Formoterol in Pure and Tablet. Asian Journal of Pharmaceutical Analysis. 2026; 16(2):89-3. doi: 10.52711/2231-5675.2026.00012
Cite(Electronic):
M. Venkata Ramana, Podishetty Akhila, Salla Pujitha, Akula Ganesh, Yerolla Soundarya. Reverse Phase High Performance Liquid Chromatography Method for Simultaneous Estimation of Budesonide and Formoterol in Pure and Tablet. Asian Journal of Pharmaceutical Analysis. 2026; 16(2):89-3. doi: 10.52711/2231-5675.2026.00012 Available on: https://ajpaonline.com/AbstractView.aspx?PID=2026-16-2-2
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