Author(s): Mahesh. M, Sridhar Thandra, Shaik Muneer, B. Siva Sai Kiran, H. Mamatha

Email(s): meghavath9@gmail.com

DOI: Propranolol, Hydralazine, RP-HPLC.   

Address: Mahesh. M1*, Sridhar Thandra2, Shaik Muneer3, B. Siva Sai Kiran3, H. Mamatha3
1,3Department of Pharmaceutical Analysis, JNTUA - Oil Technological and Pharmaceutical Research Institute, Ananthapuramu, A.P, India.
2Department of Bioanalysis, ABC Biologics, Hyderabad, Telangana, India
*Corresponding Author

Published In:   Volume - 9,      Issue - 1,     Year - 2019


ABSTRACT:
The method developed and validation was carried out by using isocratic mode with simple mobile phase preparation and separation was achieved on Phenomenex Luna C18 column (250 X 4.6 mm, 5 µm) using the mobile phase consisting of Methanol: water in the ratio of 40:60 (0.01%O-phosphoric acid). The mobile phase was pumped at a flow rate of 1.0 mL/min and detection was done by UV detector at 293 nm. The retention times were 2.76 min for Propranolol and 4.69 min for Hydralazine. The linearity ranges for Propranolol and Hydralazine were 10-60 µg/ml and 6-36 µg/ml respectively with correlation coefficient 0.999. The LOD and LOQ values of propranolol were 0.099µg/ml and 0.300µg/ml obtained and then hydralazine LOD and LOQ values were 0.093µ/ml and 0.281µg/ml respectively. This method can be applicable for routine analysis which shows good reproducible, precise and accurate for simultaneous estimation of propranolol and hydralazine in pharmaceutical dosage forms.


Cite this article:
Mahesh. M, Sridhar Thandra, Shaik Muneer, B. Siva Sai Kiran, H. Mamatha. Method Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Propranolol and Hydralazine in Pharmaceutical Dosage Form. Asian J. Pharm. Ana. 2019; 9(1): 37-42. doi: Propranolol, Hydralazine, RP-HPLC.


Recomonded Articles:

Author(s): Sushil D. Patil, Sunil V. Amurutkar, C.D. Upasani

DOI: 10.5958/2231-5675.2016.00030.2         Access: Open Access Read More

Author(s): Kirthi A, Shanmugam R, Mohana Lakshmi S , Ashok Kumar CK, Padmini K, Shanti Prathyusha M, Shilpa V

DOI:         Access: Open Access Read More

Author(s): Hamid Khan, Javed Ali

DOI: 10.5958/2231-5675.2017.00020.5         Access: Open Access Read More

Author(s): Pinaz A. Kasad, K.S. Muralikrishna

DOI:         Access: Open Access Read More

Author(s): G. Krishnamoorthy, C. Diana Priyadarshini, R. Senthamarai

DOI:         Access: Open Access Read More

Author(s): Paras Virani, Rajanit Sojitra, Hasumati Raj, Vineet Jain

DOI: 10.5958/2231-5675.2015.00025.3         Access: Open Access Read More

Author(s): P. Janaki Pathi, N. Appala Raju, G.Parvathalu

DOI:         Access: Open Access Read More

Author(s): Saroj Kumar Raul, Bukkuru Spandana, Patibandla Sameera, Vegiraju Vikitha

DOI:         Access: Open Access Read More

Author(s): Mansi J. Chaudhari, Suraj R. Chaudhari, Shailesh S. Chalikwar, Atul A. Shirkhedkar

DOI: 10.5958/2231-5675.2018.00008.X         Access: Open Access Read More

Author(s): S. Vidyadhara, RLC Sasidhar, B. Venkateswara Rao, P. Ratna Kumari

DOI:         Access: Open Access Read More

Author(s): Khushboo Patel*, Dhara Patel, Jaya Dwivedi, Priyanka Newal, Dhananjay Meshram

DOI: 10.5958/2231-5675.2017.00039.4         Access: Open Access Read More

Author(s): B. Thangabalan, M. Salomi, N. Sunitha, S. Manohar Babu

DOI:         Access: Open Access Read More

Author(s): Madhavi R., Mohana Krishna A., Shobha Rani G., Mounika D.

DOI: 10.5958/2231-5675.2015.00008.3         Access: Open Access Read More

Author(s): Shobha Rani G, Lohita M, Jaya Preethi P, Madhavi R, Sunisitha B, Mounika D

DOI:         Access: Open Access Read More

Author(s): Jayshree Pawar, Sandeep Sonawane, Santosh Chhajed, Sanjay Kshirsagar

DOI: 10.5958/2231-5675.2016.00024.7         Access: Open Access Read More

Asian Journal of Pharmaceutical Analysis (AJPA) is an international, peer-reviewed journal, devoted to pharmaceutical analysis...... Read more >>>

RNI: Not Available                     
DOI: 10.5958/2231–5675 

Popular Articles


Recent Articles




Tags