Author(s):
Mahesh. M, Sridhar Thandra, Shaik Muneer, B. Siva Sai Kiran, H. Mamatha
Email(s):
meghavath9@gmail.com
DOI:
Propranolol, Hydralazine, RP-HPLC. 
Address:
Mahesh. M1*, Sridhar Thandra2, Shaik Muneer3, B. Siva Sai Kiran3, H. Mamatha3
1,3Department of Pharmaceutical Analysis, JNTUA - Oil Technological and Pharmaceutical Research Institute, Ananthapuramu, A.P, India.
2Department of Bioanalysis, ABC Biologics, Hyderabad, Telangana, India
*Corresponding Author
Published In:
Volume - 9,
Issue - 1,
Year - 2019
ABSTRACT:
The method developed and validation was carried out by using isocratic mode with simple mobile phase preparation and separation was achieved on Phenomenex Luna C18 column (250 X 4.6 mm, 5 µm) using the mobile phase consisting of Methanol: water in the ratio of 40:60 (0.01%O-phosphoric acid). The mobile phase was pumped at a flow rate of 1.0 mL/min and detection was done by UV detector at 293 nm. The retention times were 2.76 min for Propranolol and 4.69 min for Hydralazine. The linearity ranges for Propranolol and Hydralazine were 10-60 µg/ml and 6-36 µg/ml respectively with correlation coefficient 0.999. The LOD and LOQ values of propranolol were 0.099µg/ml and 0.300µg/ml obtained and then hydralazine LOD and LOQ values were 0.093µ/ml and 0.281µg/ml respectively. This method can be applicable for routine analysis which shows good reproducible, precise and accurate for simultaneous estimation of propranolol and hydralazine in pharmaceutical dosage forms.
Cite this article:
Mahesh. M, Sridhar Thandra, Shaik Muneer, B. Siva Sai Kiran, H. Mamatha. Method Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Propranolol and Hydralazine in Pharmaceutical Dosage Form. Asian J. Pharm. Ana. 2019; 9(1): 37-42. doi: Propranolol, Hydralazine, RP-HPLC.
Cite(Electronic):
Mahesh. M, Sridhar Thandra, Shaik Muneer, B. Siva Sai Kiran, H. Mamatha. Method Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Propranolol and Hydralazine in Pharmaceutical Dosage Form. Asian J. Pharm. Ana. 2019; 9(1): 37-42. doi: Propranolol, Hydralazine, RP-HPLC. Available on: https://ajpaonline.com/AbstractView.aspx?PID=2019-9-1-19