Author(s): Prem Kumar Bichala, R. Suthakaran, Ch. Shankar

Email(s): prembichala@gmail.com

DOI: 10.5958/2231-5675.2018.00037.6   

Address: Prem Kumar Bichala1*, R. Suthakaran2, Ch. Shankar3
1Associate Professor, Department of Pharmaceutical Analysis, Vijaya College of Pharmacy, Munaganoor – 501511, Hyderabad, Telangana, India
2Professor and Principal, Department of Pharmaceutical Chemistry, Vijaya College of Pharmacy, Munaganoor – 501511, Hyderabad, Telangana, India
3Associate Professor, Department of Pharmaceutical Analysis, Vijaya College of Pharmacy, Munaganoor – 501511, Hyderabad, Telangana, India
*Corresponding Author

Published In:   Volume - 8,      Issue - 4,     Year - 2018


ABSTRACT:
A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Isoniazid and Atazanavir, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Phenomenex Gemini C18 (4.6 x 150mm, 5µm) column using a mixture of Methanol: TEA Buffer pH 4.5 (35:65) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 240 nm. The retention time of the Isoniazid and Atazanavir was 2.256, 5.427 ±0.02min respectively. The method produce linear responses in the concentration range of 5-25mg/ml of Isoniazid and 25-125mg/ml of Atazanavir. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.


Cite this article:
Prem Kumar Bichala, R. Suthakaran, Ch. Shankar. Method Development and Validation for Simultaneous Determination of Isoniaizid and Atazanavir in Pure and Pharmaceutical Formulation by using HPLC. Asian J. Pharm. Ana. 2018; 8(4): 203-208. doi: 10.5958/2231-5675.2018.00037.6


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DOI: 10.5958/2231–5675 

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