Author(s):
Atefeh Ranjbar, Ali Mohammadzadeh
Email(s):
alimohammadzadeh8888@gmail.com , a.mohammadzadeh92@bradford.ac.uk , Atefehranjbar68@gmail.com
DOI:
10.52711/2231-5675.2025.00016 
Address:
Atefeh Ranjbar1, Ali Mohammadzadeh2
1Department of Biochemistry, Faculty of Science, Payame Noor University, Bushehr, Iran.
2School of Chemistry and Lifescience, University of Bradford, Bradford BD7 1DP, UK.
*Corresponding Author
Published In:
Volume - 15,
Issue - 2,
Year - 2025
ABSTRACT:
Polycyclic Aromatic Hydrocarbons (PAHs) are toxic organic compounds that pose significant environmental contamination risks. This study aimed to screen and molecularly identifies bacteria capable of degrading phenanthrene, a representative PAH, from petroleum-contaminated soils. Samples were collected from the Arak and Bushehr oil refineries for bacterial isolation. Initial identification was based on morphological and biochemical characteristics. Ten bacterial strains were assessed for their growth on phenanthrene as the sole carbon source. The most efficient strains were further identified through 16S rRNA gene sequencing. Growth dynamics in mineral media containing phenanthrene were monitored using a spectrophotometer, while residual phenanthrene levels were quantified via High-Performance Liquid Chromatography (HPLC). The results revealed that Bacillus thuringiensis, Aeromonas caviae, and Salmonella enterica exhibited phenanthrene degradation efficiencies of 55.82%, 51.1%, and 44.7%, respectively, after 72 hours. These bacteria demonstrated both resistance to and utilization of phenanthrene as a carbon and energy source, underscoring their potential for bioremediation applications at the pilot scale.
Cite this article:
Atefeh Ranjbar, Ali Mohammadzadeh. Isolation and Identification of Phenanthrene-Degrading Bacteria from Crude Oil-Contaminated Soils around Assaluyeh Refinery: Growth Evaluation and HPLC-Based Degradation Analysis. Asian Journal of Pharmaceutical Analysis. 2025; 15(2):99-2. doi: 10.52711/2231-5675.2025.00016
Cite(Electronic):
Atefeh Ranjbar, Ali Mohammadzadeh. Isolation and Identification of Phenanthrene-Degrading Bacteria from Crude Oil-Contaminated Soils around Assaluyeh Refinery: Growth Evaluation and HPLC-Based Degradation Analysis. Asian Journal of Pharmaceutical Analysis. 2025; 15(2):99-2. doi: 10.52711/2231-5675.2025.00016 Available on: https://ajpaonline.com/AbstractView.aspx?PID=2025-15-2-5
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