Author(s):
Mounika B., Kommineni Vinutha, Rayeesa Seema, Sridevi P.
Email(s):
vinutha08.ch@gmail.com
DOI:
10.52711/2231-5675.2025.00007 
Address:
Mounika B., Kommineni Vinutha*, Rayeesa Seema, Sridevi P.
Department of Pharmaceutical Analysis, Sri Venkateshwara College of Pharmacy, Madhapur, Hyderabad, 500081, Telangana, India.
*Corresponding Author
Published In:
Volume - 15,
Issue - 1,
Year - 2025
ABSTRACT:
This study reports the method development and validation for anti-diabetic drugs by UPLC. A new, simple, rapid, selective, precise and accurate ultra-performance liquid chromatography assay has been developed for simultaneous estimation of remogliflozin, vildagliptin and metformin. The separation was achieved by using ODS 3V column with dimensions 5 µm, 4.6 mm x 250 mm. The pH of mobile phase was adjusted to 4.5 with acetonitrile. The flow rate was 0.4 mL/min, and the separated drugs were detected using UPLC detector at the wavelength of 260 nm. The method was validated as per ICH guidelines. The proposed method was found to be accurate, reproducible, and consistent. It was successfully applied for the analysis of these drugs in marketed formulations and could be effectively used for the routine analysis of formulations.
Cite this article:
Mounika B., Kommineni Vinutha, Rayeesa Seema, Sridevi P.. Development and Validation of method for Simultaneous estimation of Remogliflozin, Vildagliptin and Metformin and its validation using ICH Q2R1. Asian Journal of Pharmaceutical Analysis. 2025;15(1):40-4. doi: 10.52711/2231-5675.2025.00007
Cite(Electronic):
Mounika B., Kommineni Vinutha, Rayeesa Seema, Sridevi P.. Development and Validation of method for Simultaneous estimation of Remogliflozin, Vildagliptin and Metformin and its validation using ICH Q2R1. Asian Journal of Pharmaceutical Analysis. 2025;15(1):40-4. doi: 10.52711/2231-5675.2025.00007 Available on: https://ajpaonline.com/AbstractView.aspx?PID=2025-15-1-7
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