Author(s): Kalyani Farkade, Vikrant Salode, Tejashri Kadu

Email(s): kalyanifarkade3103@gmail.com

DOI: 10.52711/2231-5675.2023.00045   

Address: Kalyani Farkade1*, Vikrant Salode2, Tejashri Kadu3
1P.R. Pote Patil College of Pharmacy, Amaravati, MS, India.
2P.R. Patil Institute of Pharmacy, Amaravati, MS, India.
3Institute of Pharmacy and Research, Badnera, MS, India.
*Corresponding Author

Published In:   Volume - 13,      Issue - 4,     Year - 2023


ABSTRACT:
A new, simple, rapid, selective, precise and accurate isocratic reverse-phase high-performance liquid Chromatography assay method has been developed to estimate Linezolid in tablet formulations. The separation was achieved using column Inert sustain C18 (250×4.6mm, 5µ) in the mobile phase consisting of methanol: 1% Acetic acid (50:50 v/v). The flow rate was 0.80mL/min-1, and the separated Linezolid was detected using a UV detector at 249nm. Column temperature 45°C and sample temperature ambient and injection volume 20µL. The retention time of Linezolid was noted to be 7.3min. The method was validated as per ICH guidelines. The proposed method was found to be accurate, reproducible, and consistent.


Cite this article:
Kalyani Farkade, Vikrant Salode, Tejashri Kadu. Development and Validation of RP-HPLC Method for the Estimation of Linezolid from Bulk and Formulations. Asian Journal of Pharmaceutical Analysis. 2023; 13(4):273-7. doi: 10.52711/2231-5675.2023.00045

Cite(Electronic):
Kalyani Farkade, Vikrant Salode, Tejashri Kadu. Development and Validation of RP-HPLC Method for the Estimation of Linezolid from Bulk and Formulations. Asian Journal of Pharmaceutical Analysis. 2023; 13(4):273-7. doi: 10.52711/2231-5675.2023.00045   Available on: https://ajpaonline.com/AbstractView.aspx?PID=2023-13-4-9


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