Author(s): Divya Shikha, Rohit Bhatia


DOI: 10.52711/2231-5675.2023.00037   

Address: Divya Shikha1,2, Rohit Bhatia1*
1Department of Pharmaceutical Analysis, ISF College of Pharmacy, Moga -142001, Punjab, India.
2Department of Pharmacy, Usha Martin University, Angara, Ranchi - 835103, Jharkhand.
*Corresponding Author

Published In:   Volume - 13,      Issue - 4,     Year - 2023

Background: In this work, a novel, simple and accurate reverse phase high-performance liquid chromatographic method with UV detection is presented for the simultaneous estimation of Benidipineand Telmisartan in API and marketed formulations. Objective: To develop and validate this method in accordance to regulatory guidelines i.e. ICH Q2B-R1 for analytical parameters. Methods: In RP-HPLC the separation was achieved using a Xbridge C18 (250x 4.6 i.d; 5µm) column with isocratic elution using a mobile phase consisting of methanol: acetonitrile (80:20v/v/ with 0.1% v/v TEA) adjusted to pH 5.2 with glacial acetic acid at 1mL/min flow for 10min. Benidipine and Telmisartan, were detected at dual wavelength at 237 and 297nm with retention time (Rt) 6.891 and 8.997 minutes respectively. Results: Our new methods (RP-HPLC) were found to (r2>0.999) for both Benidipine and Telmisartan. The developed methods are highly selective, precise and reproducible with %RSD < 2%, Moreover the methods are accurate with recovery more than 99% in both methods. Conclusion: The RP-HPLC method results are efficient, simple and easy. These highly sensitive methods were successfully applied for assay determination of both Benidipine and Telmisartan at fixed dose combination.

Cite this article:
Divya Shikha, Rohit Bhatia. A Validated RP-HPLC Method for Simultaneous Estimation of Benidipine and Telmisartan in Bulk and Tablet Dosage Form. Asian Journal of Pharmaceutical Analysis. 2023; 13(4):231-5. doi: 10.52711/2231-5675.2023.00037

Divya Shikha, Rohit Bhatia. A Validated RP-HPLC Method for Simultaneous Estimation of Benidipine and Telmisartan in Bulk and Tablet Dosage Form. Asian Journal of Pharmaceutical Analysis. 2023; 13(4):231-5. doi: 10.52711/2231-5675.2023.00037   Available on:

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DOI: 10.5958/2231–5675 

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