Author(s): Aditya Mathur, Ravikumar Vejendla

Email(s): mathuradity@gmail.com

DOI: 10.52711/2231-5675.2023.00014   

Address: Aditya Mathur1*, Ravikumar Vejendla2
1Assistant Professor, Dept. of Pharmaceutical Chemistry, B.M College of Pharmacy, Gurugram.
2Professor, Dept. of Pharmaceutical Analysis, St. Mary Pharmacy College, Hyderabad.
*Corresponding Author

Published In:   Volume - 13,      Issue - 2,     Year - 2023


ABSTRACT:
The aim of this study is to develop and validate a method for the quantitative analysis of Rivastigmine tartarate capsules 1.5mg. An isocratic HPLC method using a reverse phase C-8 column and a mobile phase along with buffer (pH 3.0) was developed, optimized and validated. The analysis was carried out with a flow rate of 1.5 ml/min at 500C and was monitored at ?max - 220nm. Chromatogram of Rivastigmine tartarate was observed at 11min. The complete elution of Rivastigmine tartarate was achieved in 11.29 min at 220nm. This HPLC method showed good linearity, accuracy, selectivity, and other validation parameters. The recovery (accuracy) at all concentration levels was found to be more than 100% within the range of 102%. System suitability was determined by calculating the percent relative deviation (%RSD) for area five replicates injection of 120ppm in HPLC. All the peaks were resolved from the API with significantly different RT. Rivastigmine tartarate was subjected for stability indicating assay method which must be validated invariably calls for a forced degradation conditions of oxidative, acid, base, hydrolytic, thermal and photolytic degradation. Rivastigmine tartarate was found to degrade significantly in base degradation condition and little in thermal, thermal humidity, photolytic, acid and oxidative stress degradation conditions.


Cite this article:
Aditya Mathur, Ravikumar Vejendla. Novel Method Development, Validation and Stability Indicating Assay Method for Rivastigmine Tartarate Capsule by HPLC. Asian Journal of Pharmaceutical Analysis. 2023; 13(2):79-8. doi: 10.52711/2231-5675.2023.00014

Cite(Electronic):
Aditya Mathur, Ravikumar Vejendla. Novel Method Development, Validation and Stability Indicating Assay Method for Rivastigmine Tartarate Capsule by HPLC. Asian Journal of Pharmaceutical Analysis. 2023; 13(2):79-8. doi: 10.52711/2231-5675.2023.00014   Available on: https://ajpaonline.com/AbstractView.aspx?PID=2023-13-2-3


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