Author(s): Narendra Patil, Ketan Patil, Sharangouda J. Patil


DOI: 10.52711/2231-5675.2022.00045   

Address: Narendra Patil1*, Ketan Patil1, Sharangouda J. Patil2
1Department of Pharmacology/Pharmaceutics, KVPS Institute of Pharmaceutical Education, Boradi.
2Department of Zoology, NMKRV College of Women, Bengaluru.
*Corresponding Author

Published In:   Volume - 12,      Issue - 4,     Year - 2022

Nucleic acid identification, separation, purification, sequencing, diagnosis mapping, etc. are emerging field of bioscience. While designing acceptable method is crucial part of analyser. The repetitive task assigned during method the development and controlling the internal as well as external parameters may design a prominent approach for identification. The interpretation is also directed towards functional complexity of genetic disorders. The high-resolution techniques are minimize the biological barriers in recent developments and focusing on independent structure. The nucleic acid analysis is increasing in the field of bioscience and most preferable application in diagnostic laboratories at commercial scale. Identification of nucleic acid sequence will going to be applied in the analytical aspects like forensic samples, medicines, diseases identification, staging and few more environmental applications. The paper highlights the recent developments of laboratory methods in nucleic acid analysis and their commercial paradigm is necessary in current era of developmental perspectives in diagnosis field.

Cite this article:
Narendra Patil, Ketan Patil, Sharangouda J. Patil. Nucleic Acid Analytical Techniques - Current era of Development Perspectives in Diagnosis Field. Asian Journal of Pharmaceutical Analysis. 2022; 12(4):271-0. doi: 10.52711/2231-5675.2022.00045

Narendra Patil, Ketan Patil, Sharangouda J. Patil. Nucleic Acid Analytical Techniques - Current era of Development Perspectives in Diagnosis Field. Asian Journal of Pharmaceutical Analysis. 2022; 12(4):271-0. doi: 10.52711/2231-5675.2022.00045   Available on:

1.    Griffiths AJF, Miller JH, Suzuki DT. An Introduction to genetic analysis. Kolkata: W.H. Treman and Co. ;1996.
2.    Walkar JM. Methods in Molecular Biology. Clifton:Human press;1988.
3.    Matsuo T., Caprioli RM, Gross ML. Biological mass spectroscopy: Present and future. Chichester: John Willey press; 1994.
4.    Keer JT, Birch L. Essential of Nucleic acid analysis. Cambridge: RSC publishing;2008.
5.    Dr. Satyanarayana U, Dr. Chakrapani U. Biochemistry. 3rd ed. Kolkata: Book and Allied Publisher; 2010.
6.    Chawala Rajinder, Malwalli Tarek H. Text book of Medicinal Biochemistry. 1st ed. New Delhi: United India Periodicals; 2012.
7.    Chatterjea MN, Shinde Rana. Text Book Medicinal Biochemistry. 8th ed. New Delhi: Jaypee Brothers Medical Publisher; 2012.
8.    Vasudevan DM, S. Shreekumari, Vaidaynathan Kannan. Textbook of Biochemistry for medical students. 6th ed. New Delhi:Jaypee brothers medical Publisher; 2011.
9.    Minchin Steve, Lodge Julia. Understanding biochemistry:structure and function of nucleic acid. Essays in Biochem. 2018; 63:433-456.
10.    Laroy J Kricka. Nucleic acid detection technologies.clin chem .1999; 45(4): 453-458.
11.    Baynes John W, Dominiczak Marck H. Medical Biochemistry. 5th ed. Edinburgh: Elsevier; 2018.
12.    Puri Dinesh. Textbook of Medicinal Biochemistry. 6th ed. USA: Springer; 2020.
13.    Lodish H. MolecularcellBiology.4thed.NewYork:W.H.Freeman;1999.
14.    Gesteland RF. The RNAworld.2nd.NewYork: Coldspring Harbor Laboratory Press;1992.
15.    Saenger W. Principles of Nucleic acid structure.1st ed. New York: Springer-verlag;1992.
16.    Chargraff E. Structure and function of nucleic acid as cell constituents.Q.J. Microsc Sci.1951;10: 654-659.
17.    Uhlenbeck OC. Pardi A. Feigon J. RNA structure comes of age.Cell.1997;90:833-840.
18.    Daisuke M.Yoshi, Simenel Friendrich C, Marky Luis A. Synthesis and application of Nucleic acids.J. Nucleacid.2011; 2011:1-2.
19.    Watson JD, CrickFH,. Molecular structure of Nucleic acids, Nature.1953; 171(4356):737-738.
20.    Wilkins MH, Stokes AR, Wilson HR. Molecular structure of deoxypentose nucleic acid.Nature.1953; 171:738-740.
21.    Arboleda Valerie A, Xian Rena R.An over view of DNA analytical methods. Methin Mol Biol.2019;1897: 385-402.
22.    Doherty EA, Doudna JA. Ribozyme structur s and mechanisms. Rev. Biophys. Biomol. Stru. 2001; 30:457-475.
23.    Breaker Ronald R. Joyce Gerald F. The expanding view of RNA and DNA function. Chem Biol. 2004; 21(9) :1059-1065.
24.    Franklin RE. The structure of DNA and RNA. New York: Science of America; 2002.
25.    Alneida Maria J., Reis Rui M, Calin George A. microRNA history: Discovery, recent application and next frontiers. Springer. 2008; 22:1028-1042.
26.    Kryndushkin DST, Avanesyan MD. Yeast prion aggregates are formed by smalls up 35polymers fragments by HSP104. J.Bio Chem.2003; 278(49): 636-43.
27.    AryalSager. Agarose Gelelectrophoresis [Internet]. 2018. Availableform:https://microbe-notes.Com/Agarose-gel-electrophoresis/.
28.    Adamsan A.W. A hand book forgel electrophoresis. Maine: Cambrex; 2004.
29.    Dr.Sharma BK.Instrumental methods of chemical analysis.1st ed.Meerut: Krishna Prakashan;2002.
30.    Aajj C. Borst P.The gel electrophoresis of DNA.Biochem Biophys Acta.1972;2:192-200.
31.    Blot (Biology) [Internet]. Available form:
32.    Koscianska Edyta, Roslan Julia, starga Kozyzosiak Wlodzimerz J.Northern blotting analysis of micro RNA pair precursor or and RNA a interference triggers. BMC Mol Bio.2011;14: 12-14.
33.    Aryad Sagar. Southernblot-Principle steps and application Internet]. 2018. Available form:https://microbe-notes.come/southern-blot-principle-steps-andapplication/.
34.    Blotting methods and application [Internet]. Available form:
35.    Denaturing gradient gel electrophoresis [Internet]. Available form:http://wiki.biomine. Skelleffea.Se/wiki/mdex.php/DDGE.
36.    Muyzar G., Smalla K. Application of denaturing by gradient gel electrophoresis in microbioecology. AntonieJ.1998;73:127-141.
37.    Diez Beatriz, Terence Marsh, Raman Massana. Application of DDGE to study the diversity of marine picoeukaryotic assemble age and comparison of DDGE with other molecular techniques. App and Envr Microbio.2001;67: 2942-2951.
38.    Siqueria Jose, Isabela Rocas. Application DDGE to the analysis of Endotoxins infection. J of Endobios.2005; 31: 775-82.
39.    Hernango Man S, Espinosa JC. Characterization of nine yeast by TGGE, FEMS Macrobio Let,2000;193:45-50.
40.    Harmeet peters, Robbinson Peter. TGGE, Mole Diagno.2005; 32:718-720.
41.    Melissa A., Fouratt Nancy, G Love, Ann M. Application of temperature gradient gel electrophoresis to the characterization of anitrifying bioaugmentation product, MicrobioEco.2003;43(2):277-286.
42.    Bethan M Jones, Leslie A Knapp. Temporal TGGE for detection of single nucleotide polymorphism. Springer,2009;578:153-165.
43.    Hames David, Hopper Nigel. Biochemistry. 3rded. NewYork:Taylor and Francis;2005.
44.    BaileyW R, Scott E G, Boron E J. Diagnostic microbiology.St.Louis:Mosby;1986.
45.    Sastry AS, Bhat SK. Essential of Medical Microbiology. New Delhi:Jaypee Brother Medical Publisher;2016.
46.    Real time PCR [Internet]. available form:
47.    Real time PCR [Internet]. Available form:
48.    Manisa Z. Wony and Juan F. Medrano. Real time PCR form RNA quantification. Biotech.2018;39(1):207-10.
49.    DNA fingerprinting principle and technologies [Internet]. Available form: and technology of dna-fingerprinting/1221/.
50.    Gizaw S, Van Arendonk, JAM Komen. Population structure genetic variations and morphological diversity in indigenous sheep of Ethiopia. Animal Gen.2007; 38:621-628.
51.    DNA fingerprinting principle, methods, application [Internet]. Available form: lication/
52.    DNA fingerprinting [Internet]. Available form: https://www.biology exams 4u . com/2014/05/DNA fingerprinting.
53.    Koerun Viktor. Molecular markers and their application in cereals breeding.Mol Bio.2015; 12:12-28.
54.    Mahesh waran M. Molecular markers. New Delhi: IP Innovative; 2010.
55.    Choe Lella, Dutt Michael J., Kelvin H. Studies of potential cerebrospinal fluid molecular markers for Alzheimer's disease. Electropho J.2012;23(14):2247-2251.
56.    Damjan Glarae, Michael Dean. Application of heteroduplex analysis for mutation detection in disease genes. Human mutation.1995; 6(4) :201-210.
57.    J. Wallae, Andrews R. PCR mutation detection protocol. New Jersey: Human Press;2002.
58.    F. El-Yazbi Amira, Wong Aiysha. A luminescent probe of mismatched DNA hybridization. Analyti Chem Acta. 2017; 994:92-99.
59.    Tahira T., Kukita Y, Higasa K, Okazaki Y. Estimation of SNP allele frequencies by SSCP analysis of Pooled DNA. Methods in Mol Bio. 2009; 578:193-207.
60.    Michael Oto, Satoshi Miyake. Optimization of non radioisotope SSCP analysis with a conventional Gel electrophoresis apparatus. Anal Biochem .1993; 293:19-22.
61.    Kubo Karen, Stuart RM, Astua Freitas. Virus by SSCP analysis of nucleotide sequencing of a fragment from the nucleocaprid gene. Arch of Virol .2009;154(6): 1009-14.
62.    Masato Orita, Hiroyuki Lawhana. Detection of Ploymorphism of human DNA by Gel electrophoresis as SSCP Process.Nat Acta Sci.1989;86(8) :2766-2770.
63.    DNA sequencing [Internet]. Available form: https:// genetic education. come. in/dna-sequencing-hostory-steps-meyhods-application-limitations/.
64.    DNA sequencing [Internet]. Available form: sequencing.
65.    Lilian Franca, Tarso Kot. A review of DNA sequencing method. Review of Biophys. 2002; 35(2):169-200.
66.    Leharach Hans. DNA sequencing method in Human genetics and disease research. Prine Nat.2008; 28:2010-18.
67.    Sanger sequencing: Introduction, Principle and protocol [Internet]. Available form: https:// sequencing-intro-principle-protocol/.

Recomonded Articles:

Author(s): K. Pramod, Shahid H. Ansari, Javed Ali

DOI:         Access: Open Access Read More

Author(s): Yogita R. Indalkar, Aloorkar N.H.

DOI: 10.5958/2231-5675.2015.00028.9         Access: Open Access Read More

Author(s): Suraj J. Patil, Shivani D. Patil, Pratibha B. Patil, Pranali S. Patil, Ganesh B. Vambhurkar, Indryani D. Raut

DOI: 10.5958/2231-5675.2018.00040.6         Access: Open Access Read More

Author(s): Paras Virani, Rajanit Sojitra, Hasumati Raj, Vineet Jain

DOI: 10.5958/2231-5675.2015.00025.3         Access: Open Access Read More

Author(s): Rohan R. Vakhariya, Swati Talokar, Archana R. Dhole, S.K. Mohite, C.S. Magdum

DOI: 10.5958/2231-5675.2016.00001.6         Access: Open Access Read More

Author(s): Amitkumar J. Vyas, Jayshree P. Godhaniya, Ajay I. Patel, Ashok B. Patel, Nilesh K. Patel, Alpesh Chudasama, Sunny R. Shah

DOI: 10.52711/2231-5675.2021.00028         Access: Open Access Read More

Author(s): Shinde Ganesh S., P. S. Rao, R. S. Jadhav, Piyusha Kolhe, Diksha Athare

DOI: 10.5958/2231-5675.2021.00009.0         Access: Open Access Read More

Author(s): Hamid Khan, Mushir Ali, Alka Ahuja, Javed Ali

DOI:         Access: Open Access Read More

Author(s): Sharmin Reza Chowdhury, Mahfuza Maleque, Mahbubul Hoque Shihan

DOI:         Access: Open Access Read More

Author(s): K. Pramod, Shahid H. Ansari, Javed Ali

DOI:         Access: Open Access Read More

Author(s): Srinivasa Reddy, Nirmala Nayak, Imran Ahmed, Licto Thomas, Arindam Mukhopadhyay, Saral Thangam

DOI: 10.5958/2231-5675.2016.00014.4         Access: Open Access Read More

Author(s): Samer Housheh, Saleh Trefi, M. Fawaz Chehna

DOI: 10.5958/2231-5675.2017.00010.2         Access: Open Access Read More

Author(s): Gajanan Darwhekar, Priya Jain, Dinesh Kumar Jain, Gaurav Agrawal

DOI:         Access: Open Access Read More

Author(s): Priyanga Rangasamy, Vadakkenchery Salimudheen Hansiya, Palanisamy Uma Maheswari, Thamburaj Suman, Natesan Geetha

DOI: 10.5958/2231-5675.2019.00014.0         Access: Open Access Read More

Author(s): D. Narmada, P.V. Murali Krishna, Shaik Mohammad Yusuf, B. Ranganayakulu, K. Uday Praveen, P. Raja Abhilash

DOI:         Access: Open Access Read More

Asian Journal of Pharmaceutical Analysis (AJPA) is an international, peer-reviewed journal, devoted to pharmaceutical analysis...... Read more >>>

RNI: Not Available                     
DOI: 10.5958/2231–5675 

Recent Articles