ABSTRACT:
A simple, specific, sensitive, rapid, precise, accurate and economical UV Spectrophotometric simultaneous equation method have been developed and validated for the routine estimation of Dolutegravir sodium and Rilpivirine Hydrochloride in bulk form. The Method A employs estimation of drugs by simultaneous equation method (SEM) using synthetic mixture of drugs. The absorption maxima of drugs were found to be at 259.20nm for Dolutegravir sodium and 305.80nm for Rilpivirine Hydrochloride. Both drugs followed the beer lamberts law in the range of 4-24µg/ml and 1-8µg/ml for DOL and RIL respectively. Methods are validated according to ICH guidelines and can be adopted for the routine analysis Dolutegravir sodium and Rilpivirine Hydrochloride in pure bulk form.
Cite this article:
Sandip T. Thoke, Umesh T. Jadhao, Gunesh N. Dhembre. Development and Validation of UV Spectrophotometric Methods for Simultaneous Estimation of Dolutegravir Sodium and Rilpivirine Hydrochloride in Pure Bulk Form. Asian Journal of Pharmaceutical Analysis. 2022; 12(3):181-6. doi: 10.52711/2231-5675.2022.00031
Cite(Electronic):
Sandip T. Thoke, Umesh T. Jadhao, Gunesh N. Dhembre. Development and Validation of UV Spectrophotometric Methods for Simultaneous Estimation of Dolutegravir Sodium and Rilpivirine Hydrochloride in Pure Bulk Form. Asian Journal of Pharmaceutical Analysis. 2022; 12(3):181-6. doi: 10.52711/2231-5675.2022.00031 Available on: https://ajpaonline.com/AbstractView.aspx?PID=2022-12-3-5
REFERENCES:
1. Varaprasad Jagadabi, Nagendra Kumar P.V., Srinivasu P., Lanka.A.R., G. Pavani G., Int. Res. J. Pharm. 2019. 10, 1-3.
2. Nicolas Despie´gel, Delphine Anger, Monique Martin, Neerav Monga, Qu Cui, Angela Rocchi, Sonia Pulgar, Kim Gilchrist, Refoios Camejo. Infect Dis Ther. 2015. 4,337–353.
3. Gabriel Estan-Cerezo1 Ana García-Monsalve1 Leticia Soriano-Irigaray1 Francisco José Rodríguez Lucena1, Andrés Navarro-Ruiz, “A rapid validated UV-HPLC method for the simultaneous determination of the antiretroviral compounds darunavir and raltegravir in their dosage form.” Rev Esp Quimioter. Vol 3 Issue 30, 2017, pp183-194.
4. Amedeo F. Capetti1, Gaetana Sterrantino2, Maria Vittoria Cossu1, GianCarlo Orofino, Giorgio Barbarini, Giuseppe V. De Socio, Simona Di Giambenedetto, Antonio Di Biagio, Benedetto M. Celesia, Barbara Argenteri1, Giuliano Rizzardini1, “Switch to Dolutegravir plus Rilpivirine Dual Therapy in cART-Experienced Subjects: An Observational Cohort”, PLoS One Journal. Vol 10 Issue 11, 2016. pp 1-9.
5. Jomol Joseph1, N. J. R. Hepsebah, K. Deepthi, “Analytical Method Development and Validation for The Simultaneous Estimation of Dolutegravir and Rilpivirine Using Rphplc Method In Both Bulk And Pharmaceutical Dosage Form”, European Journal of Biomedical and Pharmaceutical Sciences. Vol 3 Issue 5, 2016, pp237-243.
6. Bhavar Girija Balasaheb, Aher Kiran Balasaheb, Thorat Ravindra Subhash, Kakadsachin Jijabapu, Pekamwar Sanjay Sudhakar, “Development and Validation Of UV- Spectrophotometric Method for Estimation of Dolutegravir Sodium In Tablet Dosage Form”, Malaysian Journal of Analytical Sciences. Vol 19, Issue 6, 2015, pp1156-1163.
7. Masthanamma Sk, Alekhya Gottumukkala, “Development and Validation of UV Spectrophotometric Methods for Estimation Of Rilpivirine In Bulk And Pharmaceutical Formulation”, International Journal Of Pharmaceutical Sciences And Research. Vol 5, Issue 2, 2014, pp 483-489.
8. Sonam Patel, Krishnaveni Nagappan, Gouru Santhosh Reddy, “A New Quantitative Reverse Phase High-Performance Liquid Chromatographic Method for The Quantification of Rilpivirine Hydrochloride in Bulk and Dosage Form”, Journal of Applied Pharmaceutical Science. Vol 8, Issue 11, 2018, pp157-162.
9. Tushar K. Kadia, Mr. Darshil B. Shah, Dr. Dilip G.M. “Development and Validation Of Q-Absorbance Ratio Spectrophotometric Method for Simultaneous Estimation of Cilnidipine and Metoprolol Succinate In Bulk And Combined Dosage Form”, International Journal of Pharmacy and Pharmaceutical Sciences. Vol 6, Issue 6, 2014, pp401-407.
10. P. Giriraj and T. Sivakkumar. “New Simple Spectrophotometric Method for The Simultaneous Estimation of Paracetamol and Flupirtine Maleate In Pure And Pharmaceutical Dosage Form”, Hindawi Publishing Corporation International Journal of Spectroscopy. Vol 9, Issue 2, 2014, pp1-6.
11. International Conference on Harmonization, Validation of analytical procedures: Text and methodology. ICH Harmonized Tripartite Guidelines Q2 (R1), November 2005.
12. Chirag, Amrita Parle. “Development and validation of UV spectrophotometric method for simultaneous estimation of metformin hydrochloride and alogliptin benzoate in bulk drugs and combined dosage forms “, Der Pharma Chemica. Vol 6, Issue 1, 2014. pp 303-311.
13. Mohit Rohitas, Abhinav Agrawal, Ashish K Jain, Narendra K Lariya, Anil K Kharya, Govind P. Agrawal, “Development of Simultaneous Spectrophotometric Method of Mesalazine and Prednisolone in Same Dosage Form”, International journal of Applied Pharmaceutics. Vol 2, Issue 4, 2010. pp 8-11.
14. Jasmine Chaudhary, Akash Jain, Vipin Saini, “Simultaneous Estimation of Multicomponent Formulations by Uv-Visible Spectroscopy: An Overview”, International Research Journal of Pharmacy. Vol 2, Issue 12, 2011, pp81-83.