Author(s): Vaishali P. Shelke

Email(s): Email ID Not Available

DOI: 10.52711/2231-5675.2021.00044   

Address: Vaishali P. Shelke
Saraswati Institute of Pharmacy, Kurtadi, Hingoli.
*Corresponding Author

Published In:   Volume - 11,      Issue - 4,     Year - 2021


ABSTRACT:
RP-HPLC method has been developed for the estimation of Gemigliptin and Metformin Hydrochloride. The quantification was carried out C18 bonded phase i.e. Zorbax Eclipse XDB-C18 (4.6×250mm×5µ) with particle size 5 µ/min an isocratic mode with mobile phase consisting of Methanol: Water (20:80 % v/v). The detection was carried out using UV detector at 233 nm. The solutions of Gemigliptin and Metformin Hydrochloridewere chromatographed at a constant flow rate of 1 ml/min and the retention time of the drug was found to be 2.3 min and5.8 min respectively. The linearity range of Gemigliptin and Metformin Hydrochloride were found to be from 1- 35 µg/ml of Gemigliptin and 2-18 µg/ml of Metformin Hydrochloride. Linear regression coefficient for both drugs was 0.999. As per ICH guideline the method was validated for recovery, Precision, ruggednessand linearity.


Cite this article:
Vaishali P. Shelke. Development and Validation of RP-HPLC Method for the Estimation of Gemigliptin and Metformin Hydrochloride. Asian Journal of Pharmaceutical Analysis. 2021; 11(4):259-2. doi: 10.52711/2231-5675.2021.00044

Cite(Electronic):
Vaishali P. Shelke. Development and Validation of RP-HPLC Method for the Estimation of Gemigliptin and Metformin Hydrochloride. Asian Journal of Pharmaceutical Analysis. 2021; 11(4):259-2. doi: 10.52711/2231-5675.2021.00044   Available on: https://ajpaonline.com/AbstractView.aspx?PID=2021-11-4-2


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