Author(s): Sushil D. Patil, Pravin B. Shelke, Priti Aher, Maswood Ahmed Hafizur Rahman

Email(s): sushilpharmamet@gmail.com

DOI: 10.52711/2231-5675.2021.00025   

Address: Dr. Sushil D. Patil2*, Pravin B. Shelke1*, Priti Aher1, Maswood Ahmed Hafizur Rahman1
1MET’s Institute of Pharmacy, MET League of Colleges, Bhujbal Knowledge City, Adgaon, Nashik, Maharashtra State 422003, India.
2Department of Pharmaceutical Chemistry, MET's Institute of Pharmacy, Bhujbal Knowledge City, Adgaon Nashik, Maharashtra, India.
*Corresponding Author

Published In:   Volume - 11,      Issue - 2,     Year - 2021


ABSTRACT:
A simple, rapid, economic, sensitive and precise HPLC method has been developed for the simultaneous determination of Sulphadoxine and Pyrimethamine in pharmaceutical dosage form by taking Tolterodine as an internal standard. The method was carried out using Phenomenex C18 (4.6ID × 250mm; 5µm) column and mobile phase comprised of methanol and Phosphate Buffer in proportion of ratio 60:40 v/v. The flow rate was 1.0mL/min and detection was carried out at 276nm. The retention time of Sulphadoxine, Pyrimethamine and Tolterodine were found to be 2.967, 4.058 and 6.908 respectively. Linearity of Sulphadoxine and Pyrimethamine in the range of 2 to 12µg/mL and 4 to 24µg/mL respectively. The % recoveries of Sulphadoxine and Pyrimethamine were found to be in between 99.93% to 99. 96 % respectively. The proposed method is suitable for the routine quality control analysis for simultaneous determination of Sulphadoxine and Pyrimethamine was in bulk and pharmaceutical dosage form.


Cite this article:
Sushil D. Patil, Pravin B. Shelke, Priti Aher, Maswood Ahmed Hafizur Rahman. Simultaneous determination of Sulphadoxine and Pyrimethamine by taking Tolterodine as an internal standard in Pharmaceutical dosage form by RP-HPLC. Asian Journal of Pharmaceutical Analysis. 2021; 11(2):145-0. doi: 10.52711/2231-5675.2021.00025

Cite(Electronic):
Sushil D. Patil, Pravin B. Shelke, Priti Aher, Maswood Ahmed Hafizur Rahman. Simultaneous determination of Sulphadoxine and Pyrimethamine by taking Tolterodine as an internal standard in Pharmaceutical dosage form by RP-HPLC. Asian Journal of Pharmaceutical Analysis. 2021; 11(2):145-0. doi: 10.52711/2231-5675.2021.00025   Available on: https://ajpaonline.com/AbstractView.aspx?PID=2021-11-2-15


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