Author(s): A. M. Beltagi, I. A. Lashin, W. A. Essa, A. A. Hathoot, M. Abdel Azzem

Email(s): wafaa.ahmed1992@gmail.com

DOI: 10.52711/2231-5675.2021.00011   

Address: A. M. Beltagi1, I. A. Lashin2, W. A. Essa2,3*, A. A. Hathoot3, M. Abdel Azzem3
1Department of Chemistry, Faculty of Science, Kafr El-Sheikh University, Kafr El-Sheikh, Egypt.
2Zeta Pharma for Pharmaceutical Industries, Sadat City, Menoufia Governorate, Egypt.
3Electrochemistry Laboratory, Chemistry Department, Faculty of Science, Menoufia University, Egypt.
*Corresponding Author

Published In:   Volume - 11,      Issue - 2,     Year - 2021


ABSTRACT:
A new, simple, accurate, and specific RP-HPLC stability method for determining bilastine was developed and validated. The proposed method was administered using C18 BDS Hypersil thermo column (4.6 × 250mm i.d), 5 µm particle size with a combination of potassium dihydrogen phosphate buffer pH 6.0: acetonitrile: methanol (50:25:25) as the mobile phase at a wavelength of 220nm. The retention time was 3.9 min for bilastine. The calibration plot was linear over the concentration range of 14.4–33.6µg/ml bilastine with LOD and LOQ of 0.04 and 0.11µg/ml, respectively. The technique was validated for linearity, sensitivity, accuracy, precision, and robustness. Percent recoveries were observed to be nearly 100%. The validated method was used for determining bilastine in Pharmabilast(R) tablets. The technique could be appropriate for routine evaluation at laboratories.


Cite this article:
A. M. Beltagi, I. A. Lashin, W. A. Essa, A. A. Hathoot, M. Abdel Azzem. Evolution and effectiveness of HPLC Technique for rapid estimation of an Antiallergenic agent Bilastine. Asian Journal of Pharmaceutical Analysis. 2021; 11(2):57-2. doi: 10.52711/2231-5675.2021.00011

Cite(Electronic):
A. M. Beltagi, I. A. Lashin, W. A. Essa, A. A. Hathoot, M. Abdel Azzem. Evolution and effectiveness of HPLC Technique for rapid estimation of an Antiallergenic agent Bilastine. Asian Journal of Pharmaceutical Analysis. 2021; 11(2):57-2. doi: 10.52711/2231-5675.2021.00011   Available on: https://ajpaonline.com/AbstractView.aspx?PID=2021-11-2-1


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