Author(s): S. Janet Beula, Dr. R. Suthakaran, Prem Kumar Bichala, CH. Shankar, Syed Ghouse, K. Suneetha

Email(s): Pharmjanet123@gmail.com

DOI: 10.5958/2231-5675.2020.00001.0   

Address: S. Janet Beula1*, Dr. R. Suthakaran1, Prem Kumar Bichala1, CH. Shankar1, Syed Ghouse1, K. Suneetha2
1Vijaya College of Pharmacy, Munuganoor, RR District, India.
2SSJ College of Pharmacy, Gandipet, RR District, India.
*Corresponding Author

Published In:   Volume - 10,      Issue - 1,     Year - 2020


ABSTRACT:
A simple, specific and accurate RP-HPLC method was developed for the simultaneous estimation of Bisoprolol fumerate and Hydrochlorothiazide in bulk dosage forms. The chromatographic conditions were successfully developed for the separation of Bisoprolol fumerate and Hydrochlorothiazide by using ACE C18 column (4.6×150mm) 5µ, flow rate was 1.2ml/min mobile phase ratio was (70:30 v/v) methanol: Phosphate buffer pH 3 (pH was adjusted with orthophosphoric acid), detection wavelength was 240nm. The method was validated according to ICH guidelines for specificity, LOD, LOQ, Precision, Accuracy and Linearity. The method showed good reproducibility and recovery with % RSD less than 2.


Cite this article:
S. Janet Beula, Dr. R. Suthakaran, Prem Kumar Bichala, CH. Shankar, Syed Ghouse, K. Suneetha. A Prospective Validation of Hydrocholorothiazide and Bisoprolol Fumerate in its Pure and Bulk Dosage Forms by using RP- HPLC Technique. Asian J. Pharm. Ana. 2020; 10(1):01-06. doi: 10.5958/2231-5675.2020.00001.0


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DOI: 10.5958/2231–5675 

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