Absorption ratio or iso absorptive point method has been applied for simple, sensitive, spectrophotometric analysis in UV region for the simultaneous estimation of rutin and resveratrol in combined liposomes formulation. According to this method, the ratio of absorbance at any two wavelengths of the substance, which obeys Beer’s law is constant and the value is independent of concentration and path length. Simultaneous estimation of rutin and resveratrol would be required for the combination therapy of the two drugs in the form of a single formulation. However, literature survey revealed that there was no validated UV method for the estimation of rutin and resveratrol simultaneously by UV spectrophotometric method in bulk and pharmaceutical dosage form. The Standard solution of Rutin and resveratrol shows maximum absorbance at 257 nm and 306 nm respectively and the isoabsorptive point of both the drugs is found to be 267nm. Beer’s Lamberts law is obeyed in concentration range 1-20 µg/ml for rutin while for resveratrol, Beer’s Lambert law is obeyed in concentration range 1-10 µg/ml. The results of analysis have been validated statistically and by recovery study.The present study is aimed to develop simple, sensitive, precise, accurate and reliable UV method for determination of rutin and resveratrol in combined liposomes formulation.
Cite this article:
Devi Violina, Bhupen Kalita. Simultaneous UV Spectrophotometric Estimation and Validation of Rutin and resveratrol in combined dosage form by Iso absorptive point method. Asian J. Pharm. Ana. 2018; 8(3): 147-152. doi: 10.5958/2231-5675.2018.00027.3