Author(s): Janmajoy Banerjee, H. Padmalatha, Rahul, Ranabir Chanda

Email(s): ranabirchanda@gmail.com

DOI: 10.5958/2231-5675.2018.00032.7   

Address: Janmajoy Banerjee, H. Padmalatha, Rahul, Ranabir Chanda*
Gyana Jyothi College of Pharmacy, Uppal Bus Dept. Hyderabad, India
*Corresponding Author

Published In:   Volume - 8,      Issue - 3,     Year - 2018


ABSTRACT:
In our present investigation we have developed an accurate procedure to estimate Epalrestat and Pregabalin in tablet dosage form. Chromatogram column was used 250x4.6 mm. Buffer: Acetonitrile 60:40 was used as mobile phase and it was pumped through at a flow rate of 0.8 ml/min. Temperature was constant at 25°C. Wavelength was selected at 274.0 nm. Maintenance time of Epalrestat and Pregabalin were observed 2.520 min and 3.745 min. Percentage of recovery was acquired as 99.87% and 99.55% for Epalrestat and Pregabalin. Limit of Detection (LOD) were found 0.153 and 0.376 respectively for Epalrestat and Pregabalin and limit of Quantitation (LOQ) were found 0.464 and 1.139 for Epalrestat and Pregabalin respectively. Results of analysis were validated statistically. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Epalrestat and Pregabalin in tablet dosage form.


Cite this article:
Janmajoy Banerjee, H. Padmalatha, Rahul, Ranabir Chanda. Method Development and Validation for the Simultaneous Estimation of Eplarestat and Pragabalin in Bulk and Pharmaceutical Formulation by using RP-HPLC. Asian J. Pharm. Ana. 2018; 8(3): 174-180. doi: 10.5958/2231-5675.2018.00032.7


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DOI: 10.5958/2231–5675 

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