Author(s): Nidhi K. Patel, Shailesh K. Koradia

Email(s): nidhipatel037@gmail.com

DOI: 10.5958/2231-5675.2016.00013.2   

Address: Nidhi K. Patel*, Shailesh K. Koradia
Babaria Institute of Pharmacy, BITS Educational Campus, Vadodara-Mumbai NH#8, Varnama, Vadodara-391240, Gujarat India
*Corresponding Author

Published In:   Volume - 6,      Issue - 2,     Year - 2016


ABSTRACT:
A simple, rapid, precise and accurate stability-indicating, RP-HPLC method was developed and validated for simultaneous estimation of BD and FF in bulk drug and its formulation. The method has shown adequate separation of BD and FF from their degradation products. Separation was achieved on a ODS Hypersil C18 (250mm× 4.6mmi.d,5µm)column, kept at ambient temperature, using a mobile phase consisting of ammonium acetate buffer: Acetonitrile (30:70 v/v) at a flow rate of 1.5 ml/min and UV detection at 218nm. The average retention times for BD and FF were found to be 6.04min and 3.30 min respectively. BD and FF and their combination drug product were subjected to acid hydrolysis, alkali hydrolysis, oxidation, thermal and photolytic stress conditions. Validation of the method was carried out as per ICH guidelines. Linearity was established for BD and FF in the range of 30-70 and0.9-2.1µg/ml respectively. Correlation coefficient was found to be 0.9971 and 0.9984 for BD and FF respectively.


Cite this article:
Nidhi K. Patel, Shailesh K. Koradia. Development and Validation of stability indicating RP-HPLC method for the simultaneous estimation of Beclomethasone dipropionate and Formoterol fumarate in their combined pharmaceutical dosage form. Asian J. Pharm. Ana. 2016; 6(2): 83-90. doi: 10.5958/2231-5675.2016.00013.2


Recomonded Articles:

Author(s): Sushil D. Patil, Sunil V. Amurutkar, C.D. Upasani

DOI: 10.5958/2231-5675.2016.00030.2         Access: Open Access Read More

Author(s): K. Pramod, Shahid H. Ansari, Javed Ali

DOI:         Access: Open Access Read More

Author(s): Santosh Gada, Anand Kumar Y, Saritha, C. Mallikarjuna Setty

DOI: 10.5958/2231-5675.2018.00036.4         Access: Open Access Read More

Author(s): Kirthi A, Shanmugam R, Mohana Lakshmi S , Ashok Kumar CK, Padmini K, Shanti Prathyusha M, Shilpa V

DOI:         Access: Open Access Read More

Author(s): Sushil D. Patil, Sayali K. Chaure, Maswood Ahmed Hafizur Rahman, Prajkta U. Varpe, Sanjay Kshirsagar.

DOI: 10.5958/2231-5675.2017.00004.7         Access: Open Access Read More

Author(s): Pinaz A. Kasad, K.S. Muralikrishna

DOI:         Access: Open Access Read More

Author(s): P. Janaki Pathi, N. Appala Raju, G.Parvathalu

DOI:         Access: Open Access Read More

Author(s): B. Thangabalan, M. Salomi, N. Sunitha, S. Manohar Babu

DOI:         Access: Open Access Read More

Author(s): Mansi J. Chaudhari, Suraj R. Chaudhari, Shailesh S. Chalikwar, Atul A. Shirkhedkar

DOI: 10.5958/2231-5675.2018.00008.X         Access: Open Access Read More

Author(s): Saroj Kumar Raul, Bukkuru Spandana, Patibandla Sameera, Vegiraju Vikitha

DOI:         Access: Open Access Read More

Author(s): S. Vidyadhara, RLC Sasidhar, B. Venkateswara Rao, P. Ratna Kumari

DOI:         Access: Open Access Read More

Author(s): Sri Lakshmi D, Jane T Jacob, Srinivasa Sastry D, Satyanarayana D

DOI: 10.5958/2231-5675.2017.00005.9         Access: Open Access Read More

Author(s): Yogita R. Indalkar, Aloorkar N.H.

DOI: 10.5958/2231-5675.2015.00028.9         Access: Open Access Read More

Asian Journal of Pharmaceutical Analysis (AJPA) is an international, peer-reviewed journal, devoted to pharmaceutical analysis...... Read more >>>

RNI: Not Available                     
DOI: 10.5958/2231–5675 

Popular Articles


Recent Articles




Tags