Sobhy M. El-Adl, Abdallah A. El-Shanawany, Lobna M. Abdel-Aziz, Ali F. Hassan
Sobhy M. El-Adl1, Abdallah A. El-Shanawany1, Lobna M. Abdel-Aziz1, Ali F. Hassan2*
1Department of Medicinal Chemistry, Faculty of Pharmacy, Zagazige University, Zagazig, Egypt.
2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Al-Azhar University, Assuit, Egypt.
Volume - 4,
Issue - 3,
Year - 2014
An isocratic HPLC method had been developed for rapid simultaneous separation and determination of three ceohalosporins including Cefepime, Cefotriaxone and Cefotaxime in pure form or in presence of some impurities within 8 minutes. Separation was carried out on a Hypersil gold C18 (10um, 100x4.6mm) column. Effect of pH and composition of mobile phase was studied. Beer’s law was obeyed in the range of 1-70 µg/ml for all drugs. The method was applied for the determination of drugs in both bulk and pharmaceutical forms and was validated when obtained results were compared with reference methods.
Cite this article:
Sobhy M. El-Adl, Abdallah A. El-Shanawany, Lobna M. Abdel-Aziz, Ali F. Hassan. HPLC Determination of Three Cephalosporins (Cefepime, Cefotriaxone and Cefotaxime) in Their Bulk and Dosage Forms. Asian J. Pharm. Ana. 4(3): July-Sept. 2014; Page 91-97.