Volume No. :   9

Issue No. :  4

Year :  2019

ISSN Print :  2231-5667

ISSN Online :  2231-5675


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High Performance Liquid Chromatography – A Validation View



Address:   Punam S. Desai*, Rajeshwar V. Chavan, Nikita B. Amane, Sanmati D. Shete, Archana R. Dhole
Rajarambapu College of Pharmacy, Kasegaon, Dist – Sangli, Maharashtra, India – 415404.
*Corresponding Author
DOI No: 10.5958/2231-5675.2019.00040.1

ABSTRACT:
High-performance liquid chromatography is an analytical separation technique used for most uses, because its reproductiveness, sensitivity, and the utility to differentiate non-volatile species makes the method ideal for accurate certified visualization. Chromatography is defined as a set of technologies that is used to separate the constituents of the mixture. This technique includes two steps stationary and mobile steps. The division of components is based on the difference between the splitting properties of the two layers. The division of components is based on the difference between the splitting properties of the two layers. The proposed method was to separate, validate and identify compounds, simple, accurate, and fast. Analytical method development and validation play an important role in the research and creation of pharmaceuticals. These methods are used to ensure the performance, accuracy, efficiencies and performance of drug products. Different types of detectors used to identify the peak during the development of the system. Pharmaceutical analytical chemistry deals with new analytical techniques and analytical chemists use chemistry, physics, biology, engineering, computer science, etc. to develop analytical methods. Different principles related to such interstate sciences should be considered. For example, analytical instruments like physicists developed mass spectrometer have found excellent applications in pharmaceutical analysis. High-performance liquid chromatography is an analytical separation technique used for most uses, because its reproductiveness, sensitivity, and the utility to differentiate non-volatile species make the method ideal for accurate certified visualization. Chromatography is defined as a set of technologies that is used to separate the constituents of the mixture. This technique includes two steps stationary and mobile steps. The division of components is based on the difference between the splitting properties of the two layers. The division of components is based on the difference between the splitting properties of the two layers. The proposed method was to separate, validate and identify compounds, simple, accurate, and fast. Analytical method development and validation play an important role in the research and creation of pharmaceuticals. These methods are used to ensure the performance, accuracy, efficiencies and performance of drug products. Different types of detectors used to identify the peak during the development of the system. Pharmaceutical analytical chemistry deals with new analytical techniques and analytical chemists use chemistry, physics, biology, engineering, computer science, etc. to develop analytical methods. Different principles related to such interstate sciences should be considered. For example, analytical instruments like physicists developed mass spectrometer have found excellent applications in pharmaceutical analysis.
KEYWORDS:
high performance liquid chromatography. limit of detection, limit of quantification.
Cite:
Punam S. Desai, Rajeshwar V. Chavan, Nikita B. Amane, Sanmati D. Shete, Archana R. Dhole. High Performance Liquid Chromatography – A Validation View.Asian J. Pharm. Ana. 2019; 9(4):232-236.
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