Volume No. :   9

Issue No. :  4

Year :  2019

ISSN Print :  2231-5667

ISSN Online :  2231-5675


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Development and Validation of Q-absorbance Ratio method for Simultaneous Estimation of Teniligliptin hydrobromide and Metformin HCl in Multicomponent Dosage Form



Address:   Sonali S. Gadge1*, Ashish D. Nakod2, Vaishali P. Wasnik2, Tilottama M. Gatkine2, Shrutika S. Zile2, Najuka H. Mohije2, Vikrant L. Salode2
1Assistant Professor, P. R. Patil Institute of Pharmacy, Talegaon (S.P.), Wardha- 442202
2P. R. Patil Institute of Pharmacy, Talegaon (S.P.), Wardha- 442202
*Corresponding Author
DOI No: 10.5958/2231-5675.2019.00036.X

ABSTRACT:
The main aim of this study was focuses on the development and validation of q - absorbance ratio method for simultaneous estimation of Teniligliptin hydrobromide and Metformin HCL in multicomponent dosage form with the main and important objective was to develop and validate the method for accuracy results. A simple, accurate, sensitive and precise UV- spectrophotometric (Q-Absorbance ratio) method for the simultaneous estimation of Teniligliptin Hydrobromide and Metformin hydrochloride in multicomponent dosage form was developed and validated for linearity and range, accuracy, precision, limit of detection and limit of quantitation. Q-Absorbance ratio method is employed for simultaneous estimation of Teniligliptin hydrobromide and Metformin hydrochloride from multi component dosage forms. In this method, the absorbance was measured at 241.63 nm for Teniligliptin hydrobromide and 231.65 nm for Metformin hydrochloride. Linearity for detector response was observed in range of 0.5–2.5 µg/ml and 12.5–62.5µg/ml for Teniligliptin hydrobromide and Metformin HCl respectively. Recovery studies confirmed accuracy of proposed method and results were validated as per ICH guidelines. The % RSD values were found to be less than 2 indicates good precision and reproducibility of the method. The method can be used for routine quality control of pharmaceutical formulation containing Teniligliptin hydrobromide hydrate and Metformin hydrochloride.
KEYWORDS:
Teniligliptin, Metformin, UV Spectrophotometry, Q- Absorbance ratio method, method validation.
Cite:
Sonali S. Gadge, Ashish D. Nakod, Vaishali P. Wasnik, Tilottama M. Gatkine, Shrutika S. Zile, Najuka H. Mohije, Vikrant L. Salode. Development and Validation of Q-absorbance Ratio method for Simultaneous Estimation of Teniligliptin hydrobromide and Metformin HCl in Multicomponent Dosage Form. Asian J. Pharm. Ana. 2019; 9(4):215-218.
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