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Asian Journal of Pharmaceutical Analysis
ISSN: 2231-5675(Online), 2231-5667(Print)
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Development and Validation of UV Spectroscopic by Q-absorption Ratio, RP-HPLC Method for Simultaneous Estimation of Atazanvir and Ritonavir in bulk and Pharmaceutical Dosage Form
K. Vijaya Sri
P. V. Aishwarya
K. Vijaya Sri*, S. Deepthi, M. Madhuri, P. V. Aishwarya
Department of Pharmaceutical Analysis, Malla Reddy College of Pharmacy, (Affiliated to Osmania University) Maisammaguda, Secunderabad-500 100
The main objectives of presented work were developed and validate the UV-spectrophotometric by Q-absorption ratio and RP-HPLC method for the simultaneous estimation of Atazanavir and Ritonavir as per ICH guidelines in bulk and pharmaceutical dosage form. In UV-spectrophotometric, RP-HPLC method was developed by using Acetonitrile used as a solvent and Acetonitrile/ acetate buffer in the ratio of 60:40(%v/v) used as a mobile phase. The RP-HPLC method was developed in Eclipse C18 column (100 mm × 4.6 mm, 3.5 µm particle size). In UV-spectrophotometric method, the ?max of Atazanavir and Ritonavir was 250 and 239.4nm (iso absorption point) and for HPLC –UV detection at 250 nm were found to be respectively. It was proved that linearity in the concentration range of 5–35 µg/ml for Atazanavir, 10-60 µg/ml for Ritonavir and for HPLC the linearity range between 3-150µg/ml for Atazanavir and Ritonavir 1-50 µg/ml with a correlation coefficient value of 0.999. The % RSD for intraday precision and interday precision was less than 2% for UV-spectrophotometric and RP-HPLC method. A new, simple, accurate UV-spectrophotometric and RP-HPLC method were developed for the simultaneous estimation of Atazanavir and Ritonavir in the bulk and in the pharmaceutical dosage form.
Atazanavir, Ritonavir, Q-absorption ratio, RP-HPLC and ICH.
K. Vijaya Sri, S. Deepthi, M. Madhuri, P. V. Aishwarya. Development and Validation of UV Spectroscopic by Q-absorption Ratio, RP-HPLC Method for Simultaneous Estimation of Atazanvir and Ritonavir in bulk and Pharmaceutical Dosage Form. Asian J. Pharm. Ana. 2019; 9(3):138-150.
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