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Asian Journal of Pharmaceutical Analysis
ISSN: 2231-5675(Online), 2231-5667(Print)
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Development and Validation of UV–Spectrophotometric method for the Determination of Enrofloxacin in Synthetic form and Veterinary Injectible Dosage forms
Tasneem O. ElmahadiI
Shaza W. A Shantier
Mohamed E. Adam
Tasneem O. ElmahadiI2, Shaza W. A Shantier1 and Mohamed E. Adam1*
1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Khartoum, Sudan
2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Omdurman Islamic University Omdurman, Sudan
Simple, sensitive and accurate spectrophotometric method was developed for the estimation of Enrofloxacin (EFC) in bulk and injection dosage form. The method was based on the measurement of EFC solution in 0.1M potassium hydroxide at 271nm. Validation was conducted in accordance to ICH guidelines. The developed method was found to be linear in the concentration range 2-10 µg/ml (r= 0.999), sensitive (LOD was found 0.127 µg/ml) and precise (RSD% values are less than 2%). Freedom from interference by the formulation excipients was proved by the good recovery percentages’ results (100.06% ± 1.12, n=3). The proposed method was successfully used to estimate EFC in laboratory mixture and in the injection dosage forms.
Enrofloxacin, spectrophotometric method, Validation, Injections.
Tasneem O. ElmahadiI, Shaza W. A Shantier, Mohamed E. Adam. Development and Validation of UV–Spectrophotometric method for the Determination of Enrofloxacin in Synthetic form and Veterinary Injectible Dosage forms. Asian J. Pharm. Ana. 2019; 9(1):11-14.
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