Volume No. :   8

Issue No. :  3

Year :  2018

ISSN Print :  2231-5667

ISSN Online :  2231-5675


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A Novel Stability Indicating RP-HPLC Assay Method Development and Validation for the Quantification of Cyamemazine Tartrate in bulk and its Pharmaceutical Dosage Form



Address:   S. Muneer1*, Hindustan Abdul Ahad2, Chandra Sekhar Kothapalli Bonnoth3
1Research Scholar, Research and Development, Jawaharlal Nehru Technological University, Anantapur 515002, AP, India.
2Professor in Department of Pharmaceutical Sciences, JNTUA- Oil Technological and Pharmaceutical Research Institute, Ananthapuramu 515002, AP, India.
3Professor in Chemistry, Director Foreign Affairs and Alumni Matters, JNTUA University, Ananthapuramu, A.P., India
*Corresponding Author
DOI No: 10.5958/2231-5675.2018.00031.5

ABSTRACT:
A simple and rapid stability-indicating reverse phase HPLC assay procedure has been developed and validated for Cyamemazine tartrate in bulk and its Pharmaceutical dosage form. Chromatographic separation was achieved on Phenomenex Luna C18 (250 mm x 4.6, 5µ particle size) column using isocratic elution with mobile phase consisting of water, Acetonitrile and Methanol (60:30:10 v/v/v) under ambient temperature at 1.0 mL min-1. The procedure showed a dynamic linear response over 10– 60 µg mL-1 demonstrating correlation coefficient (r2) above 0.999, with the limits of detection and quantitation of 1.72 and 5.23 µg mL-1 respectively. The batch injection repeatability for intra and inter assay precision and accuracy studies were within the acceptance. The analyte was subjected for various stress conditions. The procedure well separated Cyamemazine tartrate and its potential degradation products which prove the stability indicating nature of the method.
KEYWORDS:
Cyamemazine tartrate, stability-indicating, Method validation, ambient, qunatitation.
Cite:
S. Muneer, Hindustan Abdul Ahad, Chandra Sekhar Kothapalli Bonnoth. A Novel Stability Indicating RP-HPLC Assay Method Development and Validation for the Quantification of Cyamemazine Tartrate in bulk and its Pharmaceutical Dosage Form.Asian J. Pharm. Ana. 2018; 8(3): 169-173.
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