Volume No. :   8

Issue No. :  2

Year :  2018

ISSN Print :  2231-5667

ISSN Online :  2231-5675


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Application of Normal and Reversed-Phase TLC/ Densitometry Methods for Determination of Naratriptan Hydrochloride in Tablet dosage form



Address:   Atul A. Shirkhedkar, Jayshri S. Borse*, Manoj D. Patil, Amod S. Patil
Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, (M.S.), India 425 405
*Corresponding Author
DOI No: 10.5958/2231-5675.2018.00018.2

ABSTRACT:
Naratriptan Hydrochloride (NTPH) is used as antimigraine drugs. In the present investigations two simple, economical and precise, normal-phase High-Performance Thin-Layer Chromatography (Method A) and Reversed Phase High Performance Thin Layer Chromatography (Method B) have been established for determination of NTPH in bulk and in tablets. Method A and Method B were developed on aluminium plates precoated with silica gel 60F254 and RP-18 silica gel 60 F254 S as the stationary phase, respectively. Separation of NTPH was achieved using Chloroform: 2-propranol: triethylamine (8:1.4:0.6 v/v) in Method A and methanol: water: triethylamine (9:0.6:0.4 v/v) in Method B. Densitometry scanning were performed at 223 nm. Both methods were found to give compact spot for the NTPH at Rf 0.55 ± 0.02. Validation of Method A and Method B were performed as per as International Conference on Harmonization guidelines.
KEYWORDS:
TLC, Densitometry, Naratriptan Hydrochloride
Cite:
Atul A. Shirkhedkar, Jayshri S. Borse, Manoj D. Patil, Amod S. Patil. Application of Normal and Reversed-Phase TLC/ Densitometry Methods for Determination of Naratriptan Hydrochloride in Tablet dosage form. Asian J. Pharm. Ana. 2018; 8(2):91-95.
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