Volume No. :   8

Issue No. :  1

Year :  2018

ISSN Print :  2231-5667

ISSN Online :  2231-5675


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Development and Validation of UV Spectrophotometry and RP-HPLC Method for simultaneous determination of Rosuvastin and Clopidogrel in Tablet Dosage Form



Address:   Roshan Telrandhe
Kamla Nehru College of Pharmacy, Butibori, Nagpur 441108, Maharashtra, India *Corresponding Author
DOI No: 10.5958/2231-5675.2018.00005.4

ABSTRACT:
The aimed of research the method development and validation by UV spectrophotometry and RP HPLC method of the Rosuvastatin calcium and Clopidogrel bisulphate. The method is a simple, accurate, specific, precise, reproducible and sensitive. The ? max of ROSU and CLOP was found to be 240nm. The coefficient correlation 0.999, the Beer’s Law limit 50-150 µg/ml, from the four trial of different concentration of mobile phase was selected Methanol:Water 80:20 v/v, pH 3.0 at 240nm, flow rate 1ml/min, sample inlet 20 µL, C 18 Prontosil, %RSD of ROSU 1.017 and CLOP 0.173, theoretical plates ROSU 7797.53 and ROSU 8257.53, Retention time of ROSU 3.483min and CLOP 4.983min, Tailing factor ROSU 1.1787 and CLOP 1.074, limits 2 NMT, Accuracy ROSU 0.37 % RSD, Recovery 99.59% and CLOP 0.18 %RSD, recovery 100.41% was show good efficacy and results. The methods indicate future scope in analysis quality control of the estimation of ROSU and CLOP for routine drug quality analysis investigation.
KEYWORDS:
UV Spectrophotometry, RP HPLC, Rosuvastatin calcium, Clopidogrel bisulphate, Simultanious estimation, validation.
Cite:
Roshan Telrandhe. Development and Validation of UV Spectrophotometry and RP-HPLC Method for simultaneous determination of Rosuvastin and Clopidogrel in Tablet Dosage Form. Asian J. Pharm. Ana. 2018; 8(1): 25-32.
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