Volume No. :   7

Issue No. :  3

Year :  2017

ISSN Print :  2231-5667

ISSN Online :  2231-5675


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Development and validation of RP-HPLC method for simultaneous estimation of ezetimibe and glimepiride in tablet dosage form



Address:   Ashwini Parmar, Sandeep Sonawane*, Santosh Chhajed, Sanjay Kshirsagar
MET’s Institute of Pharmacy, Bhujbal Knowledge City, Adgaon, Nashik – 42200, India
*Corresponding Author
DOI No: 10.5958/2231-5675.2017.00029.1

ABSTRACT:
A simple, accurate and precise RP-HPLC method was developed and validated for simultaneous estimation of glimepiride (GLM) and ezetimibe (EZE) in tablet dosage form. Both drugs were separated on C18 Phenomenex column (250 × 4.6 mm, 5 µ) using methanol: 20mM potassium phosphate buffer (pH 3.00) (80: 20 % v/v) at flow rate of 1 mL/min. All eluents were detected at 230 nm. GLM and EZE were eluted at 5.292 and 4.183 min, respectively. The method was linear in the range of 2-10 µg/mL and 20-100 µg/mL for GLM and EZE, respectively. The developed method was further validated as per ICH Q2(R1) guidelines and applied successfully for the quantitation of tablet dosage form.
KEYWORDS:
Ezetimibe and Glimepiride, RP-HPLC, Analytical Method Validation.
Cite:
Ashwini Parmar, Sandeep Sonawane, Santosh Chhajed, Sanjay Kshirsagar. Development and validation of RP-HPLC method for simultaneous estimation of ezetimibe and glimepiride in tablet dosage form. Asian J. Pharm. Ana. 2017; 7(3): 185-188.
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