Volume No. :   7

Issue No. :  2

Year :  2017

ISSN Print :  2231-5667

ISSN Online :  2231-5675


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Validated UPLC/Q-TOF-MS Method for Simultaneous Determination of Aceclofenac, Paracetamol and Chlorzoxazone in Human Plasma and its Application to Pharmacokinetic Study



Address:   Hamid Khan1* Mushir Ali2, Alka Ahuja2, Javed Ali2
1JK College of Pharmacy, Bilaspur, CG, India-495001
2Department of Pharmaceutics, Faculty of Pharmacy, Hamdard University, New Delhi, India-110062
*Corresponding Author
DOI No: 10.5958/2231-5675.2017.00016.3

ABSTRACT:
In the presented work the ultra-performance liquid chromatographic/quadrupole time-of-flight mass spectrometric (UPLC/Q-TOF-MS) method has been developed for pharmacokinetic study of some anti-inflammatory and analgesic drugs in human plasma. The aceclofenac, paracetamol, and chlorzoxazone were analyzed by Acquity UPLC BEH C18 (100.0_2.1 mm, 1.7 lm) column using isocratic mobile phase consisting of acetonitrile-2mM ammonium acetate (50:50, v/v) at a flow rate of 0.20 mL/min. The Q-TOF mass spectrometer was operated in positive ionization mode and quantization was done using the MS/MS transitions m/z 354.07 to 215.07 for aceclofenac, 152.07 to 110.06 for paracetamol and 170.00 to 134.00 for chlorzoxazone. The calibration curves were linear over the concentration range of 1–1000 ng/mL for all the drugs. The developed method was validated according to ICH guidelines. The method was applied for pharmacokinetic study of tablets containing aceclofenac, paracetamol, and chlorzoxazone in human plasma.
KEYWORDS:
UPLC/Q-TOF-MS, Aceclofenac, Paracetamol, Chlorzoxazone, Pharmacokinetic Study.
Cite:
Hamid Khan, Mushir Ali, Alka Ahuja, Javed Ali. Validated UPLC/Q-TOF-MS Method for Simultaneous Determination of Aceclofenac, Paracetamol and Chlorzoxazone in Human Plasma and its Application to Pharmacokinetic Study. Asian J. Pharm. Ana. 2017; 7(2): 93-99.
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