Volume No. :   6

Issue No. :  4

Year :  2016

ISSN Print :  2231-5667

ISSN Online :  2231-5675


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Simultaneous UV Spectrophotometric Method for the Determination of Tenofovir, Efavirenz and Lamivudine in Bulk and Combined Dosage Form



Address:   S. Vidyadhara, RLC Sasidhar*, B. Venkateswara Rao, P. Ratna Kumari
Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram, Guntur – 19, Andhra Pradesh, India.
*Corresponding Author
DOI No:

ABSTRACT:
The aim of the present work is to develop a simple, accurate, precise and reproducible UV spectrophotometric method for the simultaneous determination of Tenofovir, Efavirenz and Lamivudine in bulk and pharmaceutical dosage form. The stock solutions of the standard drugs were prepared in methanol followed by the further required dilutions with distilled water. This method involves the formation and solving of simultaneous equations at 260nm, 347nm and 272 nm, as absorbance maxima of Tenofovir, Efavirenz and Lamivudine respectively. Beer’s law obeyed the concentration range of 10 – 40mcg/mL, 5 – 20 mcg/mL and 5 – 20 mcg/mL for Tenofovir, Efavirenz and Lamivudine respectively. The results of analysis were validated statistically and by recovery studies. The % RSD for the recovery study was less than 2. The proposed method can be effectively used for the simultaneous estimation of these three drugs in bulk and combined tablet dosage form.
KEYWORDS:
Tenofovir, Efavirenz and Lamivudine, Simultaneous equation method, Method validation.
Cite:
S. Vidyadhara, RLC Sasidhar, B. Venkateswara Rao, P. Ratna Kumari. Simultaneous UV Spectrophotometric Method for the Determination of Tenofovir, Efavirenz and Lamivudine in Bulk and Combined Dosage Form. Asian J. Pharm. Ana. 2016; 6(4): 253-258.
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